Recombinant construct P{UAS-sgg.B}

General Information
Symbol	P{UAS-sgg.B}	FlyBase ID	FBtp0002654
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	4 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Bourouis, 1999.6.7)
Uses	CV term Qualifiers & info Reference binary system (targeted) sgg (Bourouis, 1999.6.7) characterization sgg (Bourouis, 1999.6.7)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Bourouis, 1999.6.7) chimeric gene (Bourouis, 1999.6.7) selectable marker (Bourouis, 1999.6.7)
Component Alleles
Allele	sgg^Scer\UAS.cBa
Reference(s)	(Bourouis, 2002, Weihe et al., 2004, Katanaev et al., 2005, Bourouis, 1999.6.7, Yuan et al., 2005, Yuan et al., 2005, Cyran et al., 2005, Fang et al., 2007, Acebes et al., 2012, Kiger et al., 2007, Yang et al., 2012, Terriente et al., 2008, Martin-Pena et al., 2006, Ataman et al., 2008, Wolf et al., 2007, Acebes et al., 2011, Morris et al., 2012, van Eyk et al., 2011, Galletti et al., 2009, Morris et al., 2008)
Molecular data	Scer\UAS sequences drive expression of the sgg10 isoform. (Martinek et al., 2001) Construct: Expression of sgg coding sequence is governed by Scer\UAS regulatory sequences. (Bourouis, 1999.6.7)
Phenotypic class	behavior defective, with Scer\GAL4^elav-C155 (Wolf et al., 2007) circadian rhythm defective, with Scer\GAL4^tim.PE (Yuan et al., 2005, Wolf et al., 2007) lethal \| pharate adult stage, with Scer\GAL4^ey.PH (Morris et al., 2012) lethal \| pharate adult stage, with Scer\GAL4^GMR.PF (Morris et al., 2012) lethal \| pupal stage, with Scer\GAL4^ey.PH (Morris et al., 2012) lethal \| pupal stage, with Scer\GAL4^GMR.PF (Morris et al., 2012) locomotor rhythm defective, with Scer\GAL4^tim.Scer\UAS (Martinek et al., 2001, Fang et al., 2007) neuroanatomy defective, with Scer\GAL4⁷⁹⁶ (Martin-Pena et al., 2006) neuroanatomy defective, with Scer\GAL4^da.G32 (Martin-Pena et al., 2006) neuroanatomy defective, with Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) neuroanatomy defective, with Scer\GAL4^GH298 (Acebes et al., 2011) neuroanatomy defective, with Scer\GAL4^NP1227 (Acebes et al., 2011) neuroanatomy defective, with Scer\GAL4^Sxl-NP2426 (Acebes et al., 2011) neuroanatomy defective, with Scer\GAL4^Sxl-NP2426, Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) neurophysiology defective \| larval stage, with Scer\GAL4^futsch-C380 (Ataman et al., 2008) short lived, with Scer\GAL4^tim.Scer\UAS (Martinek et al., 2001) smell perception defective, with Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) smell perception defective, with Scer\GAL4^GH298 (Acebes et al., 2011) smell perception defective, with Scer\GAL4^GH298, Scer\GAL80^ts.αTub84B (Acebes et al., 2011) smell perception defective, with Scer\GAL4^Sxl-NP2426 (Acebes et al., 2011) viable, with Scer\GAL4^GMR.PFa (van Eyk et al., 2011) visible, with Scer\GAL4^455.2 (Bourouis, 2002) visible, with Scer\GAL4^bi-md653 (Bourouis, 2002) visible, with Scer\GAL4^C-765 (Yang et al., 2012) visible, with Scer\GAL4^GMR.PFa (van Eyk et al., 2011) visible, with Scer\GAL4^sca-537.4 (Bourouis, 2002) visible, with Scer\GAL4^vg.PM (Bourouis, 2002) visible \| pharate adult stage, with Scer\GAL4^ey.PH (Morris et al., 2012) visible \| pharate adult stage, with Scer\GAL4^GMR.PF (Morris et al., 2012)
Phenotype manifest in	adult thorax & chaeta, with Scer\GAL4^sca-537.4 (Bourouis, 2002) adult thorax & microchaeta, with Scer\GAL4^sca-537.4 (Bourouis, 2002) antennal lobe, with Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) antennal lobe, with Scer\GAL4^GH298 (Acebes et al., 2011) antennal lobe, with Scer\GAL4^NP1227 (Acebes et al., 2011) antennal lobe, with Scer\GAL4^Sxl-NP2426 (Acebes et al., 2011) antennal lobe, with Scer\GAL4^Sxl-NP2426, Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) antennal lobe, with Scer\GAL4^Tab2-201Y (Acebes et al., 2011) antennal lobe glomerulus DM2, with Scer\GAL4^GH298 (Acebes et al., 2012) embryonic/larval neuromuscular junction, with Scer\GAL4^futsch-C380 (Ataman et al., 2008) eye, with Scer\GAL4^GMR.PFa (van Eyk et al., 2011) eye \| pharate adult stage, with Scer\GAL4^ey.PH (Morris et al., 2012) eye \| pharate adult stage, with Scer\GAL4^GMR.PF (Morris et al., 2012) head \| pharate adult stage, with Scer\GAL4^ey.PH (Morris et al., 2012) head \| pharate adult stage, with Scer\GAL4^GMR.PF (Morris et al., 2012) intrinsic neuron, with Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) intrinsic neuron, with Scer\GAL4^GH298 (Acebes et al., 2011) intrinsic neuron, with Scer\GAL4^NP1227 (Acebes et al., 2011) intrinsic neuron, with Scer\GAL4^Sxl-NP2426 (Acebes et al., 2011) intrinsic neuron, with Scer\GAL4^Sxl-NP2426, Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) intrinsic neuron, with Scer\GAL4^Tab2-201Y (Acebes et al., 2011) mesothoracic tergum, with Scer\GAL4^455.2 (Bourouis, 2002) mushroom body, with Scer\GAL4^Mef2.247 (Acebes et al., 2012) mushroom body, with Scer\GAL4^Tab2-201Y (Acebes et al., 2012) posterior dorsocentral bristle, with Scer\GAL4^C-765 (Yang et al., 2012) scutellar bristle, with Scer\GAL4^455.2 (Bourouis, 2002) scutellum, with Scer\GAL4^455.2 (Bourouis, 2002) synapse, with Scer\GAL4⁷⁹⁶ (Martin-Pena et al., 2006) synapse, with Scer\GAL4^da.G32 (Martin-Pena et al., 2006) synapse, with Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) synapse, with Scer\GAL4^GH298 (Acebes et al., 2011) synapse, with Scer\GAL4^NP1227 (Acebes et al., 2011) synapse, with Scer\GAL4^Sxl-NP2426 (Acebes et al., 2011) synapse, with Scer\GAL4^Sxl-NP2426, Scer\GAL4^{exba-krasavietz} (Acebes et al., 2011) synapse, with Scer\GAL4^Tab2-201Y (Acebes et al., 2011) wing, with Scer\GAL4^30A (Kiger et al., 2007) wing, with Scer\GAL4^bi-md653 (Bourouis, 2002) wing disc, with Scer\GAL4^455.2 (Bourouis, 2002) wing margin, with Scer\GAL4^vg.PM (Bourouis, 2002) wing margin bristle, with Scer\GAL4^sca-537.4 (Bourouis, 2002) wing margin bristle, with Scer\GAL4^vg.PM (Bourouis, 2002)
Other information

Allele	w^+mC
Reference(s)	(Bourouis, 1999.6.7)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 4 )
Bloomington	5361 w¹¹¹⁸; P{UAS-sgg.B}MB5 5435 w¹¹¹⁸; P{UAS-sgg.B}MB14/CyO
Kyoto	108186 w¹¹¹⁸; P{UAS-sgg.B}MB5 108233 w¹¹¹⁸; P{UAS-sgg.B}MB14/CyO
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	(uas)-sggB (Cyran et al., 2005) P{UAS-sgg.B} (FlyBase, 1996-) P{UAS-sggB} (Morris et al., 2012) UAS-GSK3 (Martin-Pena et al., 2006, Acebes et al., 2011, Acebes et al., 2012) UAS-sgg (Katanaev et al., 2005, Weihe et al., 2004, Fang et al., 2007, Morris et al., 2008) UASsgg (Terriente et al., 2008) UAS-Sgg (Ataman et al., 2008, van Eyk et al., 2011, Galletti et al., 2009) UAS-SGG10 (Yuan et al., 2005, Yuan et al., 2005, Wolf et al., 2007) UAS-sgg(wt) (Bourouis, 2002) UAS-Sgg^WT (Yang et al., 2012) UAS-shaggy (Kiger et al., 2007) UAS-Shaggy (Acebes et al., 2011)
Secondary FlyBase IDs

References ( 20 )
Research paper	Acebes et al., 2012, J. Neurosci. 32(2): 417--422 Central Adaptation to Odorants Depends on PI3K Levels in Local Interneurons of the Antennal Lobe. [FBrf0217241] Morris et al., 2012, Genetics 191(4): 1227--1238 Divergent Functions Through Alternative Splicing: The Drosophila CRMP Gene in Pyrimidine Metabolism, Brain, and Behavior. [FBrf0219149] Yang et al., 2012, Development 139(2): 325--334 The kinase Sgg modulates temporal development of macrochaetes in Drosophila by phosphorylation of Scute and Pannier. [FBrf0216991] Acebes et al., 2011, J. Neurosci. 31(8): 2734--2745 Synapse loss in olfactory local interneurons modifies perception. [FBrf0213264] van Eyk et al., 2011, Hum. Mol. Genet. 20(14): 2783--2794 Perturbation of the Akt/Gsk3-{beta} signalling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs. [FBrf0213942] Galletti et al., 2009, Mol. Cell. Biol. 29(12): 3424--3434 Identification of domains responsible for ubiquitin-dependent degradation of dMyc by glycogen synthase kinase 3beta and casein kinase 1 kinases. [FBrf0215466] Ataman et al., 2008, Neuron 57(5): 705--718 Rapid activity-dependent modifications in synaptic structure and function require bidirectional Wnt signaling. [FBrf0204152] Morris et al., 2008, Nature 455(7212): 552--556 E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. [FBrf0221005] Terriente et al., 2008, Dev. Biol. 320(1): 102--112 The Drosophila gene zfh2 is required to establish proximal-distal domains in the wing disc. [FBrf0207390] Fang et al., 2007, Genes Dev. 21(12): 1506--1518 Post-translational regulation of the Drosophila circadian clock requires protein phosphatase 1 (PP1). [FBrf0200630] Kiger et al., 2007, Dev. Biol. 301(1): 178--191 Tissue remodeling during maturation of the Drosophila wing. [FBrf0192456] Wolf et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(11): 4653--4657 GSK-3/Shaggy regulates olfactory habituation in Drosophila. [FBrf0194519] Martin-Pena et al., 2006, J. Neurosci. 26(40): 10199--10208 Age-independent synaptogenesis by phosphoinositide 3 kinase. [FBrf0193277] Cyran et al., 2005, J. Neurosci. 25(22): 5430--5437 The double-time protein kinase regulates the subcellular localization of the Drosophila clock protein period. [FBrf0187899] Katanaev et al., 2005, Cell 120(1): 111--122 Trimeric G protein-dependent frizzled signaling in Drosophila. [FBrf0183772] Yuan et al., 2005, Neuron 47(1): 115--127 Serotonin modulates circadian entrainment in Drosophila. [FBrf0188183] Weihe et al., 2004, Development 131(4): 767--774 Proximodistal subdivision of Drosophila legs and wings: the elbow-no ocelli gene complex. [FBrf0174589] Bourouis, 2002, genesis 34(1-2): 99--102 Targeted increase in Shaggy activity levels blocks wingless signaling. [FBrf0152388]
Supplementary material	Yuan et al., 2005, Neuron 42(1): Serotonin modulates circadian entrainment in Drosophila. [FBrf0199073]
Personal communication to FlyBase	Bourouis, 1999.6.7, UAS constructs. UAS constructs. [FBrf0135967]