Recombinant construct P{Crew}

General Information
Symbol	P{Crew}	FlyBase ID	FBtp0002704
Feature type	transgenic_transposon	Created / Updated	1999-06-25/1999-06-30

Size		Expression data
Location-dependent role		Associated insertions	3 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Siegal and Hartl, 2000, Siegal and Hartl, 1996)
Uses	CV term Qualifiers & info Reference enzyme source P1\cre (Siegal and Hartl, 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference general promoter (Siegal and Hartl, 1996) selectable marker (Siegal and Hartl, 1996)
Component Alleles
P1\cre^Mos1.hs
Reference(s)	(Siegal and Hartl, 1996, Karakozova et al., 2004)
Molecular data	Construct: Fusion of an hsp70 promoter and Dmau\mariner\T^Mos1 promoter (provides translation initiation sequences) drive expression of P1\cre, polyadenylation signal sequence are donated by Dmau\mariner\T^Mos1. (Siegal and Hartl, 1996)
Phenotypic class
Phenotype manifest in
Other information
w^+mC
Reference(s)	(Siegal and Hartl, 1996)
Molecular data	Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors. 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Minigene of the white cDNA. (Chung et al., 1993) Deletion of w^+mC 5' flanking sequences to position -305. (Qian and Pirrotta, 1995)
Phenotypic class	courtship defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{CaSpeR-4} (Siegal and Hartl, 1996)
descendant(s)
Comments
	construct_comment: Note: construct shown in opposite orientation in FBrf0090798.Progenitors are those of the plasmid, pP{Crew}. (Siegal and Hartl, 1996) P{Crew} and P{Crey} insertions have high l (Siegal and Hartl, 2000) evels of baseline (non-heat-shocked) expression of P1\cre. (Siegal and Hartl, 2000)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[w⁺, cre] (Karakozova et al., 2004, Siegal and Hartl, 1996) P{@w^+mC@ @P1\cre^Mos1.hs@=@Crew@} (FlyBase, 1996-) P{Crew} (FlyBase, 1996-) P{w⁺,cre} (Siegal and Hartl, 2000)
Secondary FlyBase IDs

References ( 15 )
Research paper	Karakozova et al., 2004, Genetics 167(3): 1275--1280 The mod(mdg4) component of the Su(Hw) insulator inserted in the P transposon can repress its mobility in Drosophila melanogaster. [FBrf0179270] Giordano et al., 2002, Genetics 160(2): 637--648 RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. [FBrf0144904] Lloyd et al., 2002, Genome 45(2): 296--312 Enhancer of garnet/δAP-3 is a cryptic allele of the white gene and identifies the intracellular transport system for the white protein. [FBrf0147069] Siegal and Hartl, 2000, Methods Molec. Biol. 136: 487--495 Application of Cre/loxP in Drosophila. Site-specific recombination and transgene coplacement. [FBrf0128645] Soldatov et al., 1999, Molec. Cell. Biol. 19(5): 3769--3778 TAFII40 protein is encoded by the e(y)1 gene: biological consequences of mutations. [FBrf0108279] Hagstrom et al., 1996, Genes Dev. 10(24): 3202--3215 Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. [FBrf0091065] Hing and Carlson, 1996, J. Neurobiol. 30(4): 454--464 Male-male courtship behavior induced by ectopic expression of the Drosophila white gene: Role of sensory function and age. [FBrf0089680] Siegal and Hartl, 1996, Genetics 144(2): 715--726 Transgene coplacement and high efficiency site-specific recombination with the Cre/loxP system in Drosophila. [FBrf0090798] True et al., 1996, Genetics 142(3): 819--837 A genome-wide survey of hybrid incompatibility factors by the introgression of marked segments of Drosophila mauritiana chromosomes into Drosophila simulans. [FBrf0086664] Qian and Pirrotta, 1995, Genetics 139(2): 733--744 Dosage compensation of the Drosophila white gene requires both the X chromosome environment and multiple intragenic elements. [FBrf0080335] Dorer and Henikoff, 1994, Cell 77(7): 993--1002 Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. [FBrf0072979] Chung et al., 1993, Cell 74(3): 505--514 A 5' element of the chicken -globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. [FBrf0064408] Roseman et al., 1993, EMBO J. 12(2): 435--442 The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects. [FBrf0058161]
Review	Pirrotta, 1988, Rodriguez, Denhardt, 1988: 437--456 Vectors for P-mediated transformation in Drosophila. [FBrf0048982]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]