General Information
Symbol
P{GT1}
FlyBase ID
FBtp0002720
Feature type
transgenic_transposon
Component Allele(s)
Size
8.447
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
Construct components
Product class / Tool use(s)
Description
The P{GT1} element contains two trapping cassettes. At the 3' end of the element there is a mutagenic gene trap cassette consisting of an artificial splice acceptor site, a promoterless GAL4 driver coding sequence and an Hsp70 poly(A) signal. Upon integration into the genome, the GAL4 coding sequence can be transcribed as a fusion mRNA with an upstream exon sequence. A 'stop-start' sequence is placed between the splice acceptor site and the GAL4 coding sequence to ensure that the open reading frame of GAL4 is maintained in any integration event. At the 5' end of the element, a polyA trap cassette is present that consists of the w+mGT minigene (which has a promoter but lacks a poly(A) signal sequence) followed by an artificial splice donor site. The w+mGT mRNA is only polyadenylated upon insertion of the P{GT1} element into the intron of a host gene, upstream of an exon containing poly(A) signal sequence, and thus w expression can be used as a marker for a successful integration into an intron in the correction orientation (since in the absence of polyadenylation the mRNA is likely to be rapidly degraded). A Tn\neoRHsp70Bb.PS marker is present between the two trapping cassettes.
Component allele Tn\neoRHsp70Bb.PS
Description
Construct: Tn\neoR gene lacking the bacterial promoter but driven by 465bp Xba Xmn fragment of the Hsp70 heat shock promoter and 206 nucleotides of untranslated leader sequence.
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
(bases 1-8447 )
Segments and Size
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Reference(s)
Molecular data
Construct: Tn\neoR gene lacking the bacterial promoter but driven by 465bp Xba Xmn fragment of the Hsp70 heat shock promoter and 206 nucleotides of untranslated leader sequence.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html
Stocks (452)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (29)