Recombinant construct P{GT1}

General Information
Symbol	P{GT1}	FlyBase ID	FBtp0002720
Feature type	transgenic_transposon

Size	8.447	Expression data
Associated insertions	1013 available
Molecular map
Recent Updates
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FB2013_03	Alleles Scer\GAL4[BG02569]
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	GAL4 gene trap (Lukacsovich and Asztalos, 1999.7.15)
Description	CV term Qualifiers & info Reference transposon P-element (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001, Parks et al., 2004, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference gene trap binary system (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001) P-element (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent AB028139 (bases 1-8447)
Segments & Size
Total Size	8.447 (Lukacsovich and Asztalos, 1999.7.15)
Left end	P5\' (Lukacsovich and Asztalos, 1999.7.15)
Right end	P3\' (Lukacsovich and Asztalos, 1999.7.15)

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference gene trap (Lukacsovich and Asztalos, 1999.7.15) gene trap (Lukacsovich and Asztalos, 1999.7.15) reporter gene (Lukacsovich and Asztalos, 1999.7.15) selectable marker (Lukacsovich and Asztalos, 1999.7.15) selectable marker (Lukacsovich and Asztalos, 1999.7.15)
Component Alleles
Allele	Scer\GAL4^GT1
Reference(s)	(Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data	Scer\GAL4 coding region with a poly(A) signal sequence but lacking a promoter. The coding sequence is preceeded by an artificial splice acceptor site, which allows the Scer\GAL4 sequence to be transcribed as a fusion mRNA with an upstream exon sequence when the P{GT1} element is inserted into the genome. A "stop-start" sequence is placed between the splice acceptor site and the Scer\GAL4 gene which ensures that the Scer\GAL4 open reading frame is maintained in any integration event. (Lukacsovich et al., 2001) Construct: The Scer\GAL4 wild type coding sequences start at the ATG of the coding sequence, with 5' UTR removed. The coding sequence thus has no promoter. A synthetic oligonucleotide containing a "stop-start" signal has been placed immediately 5' of the coding region. This sequence has consecutive overlapping transcriptional stop and start codons with which otherwise variable reading frames can be set into the proper reading frame for Scer\GAL4. The frameshift sequence was designed to be similar to the functional kozak start site, to allow translation to initiate from the Scer\GAL4 ATG if an untranslated exon is spliced to the Scer\GAL4 mRNA. An artificial splice acceptor site was engineered to be 5' of the "stop-start" signal, and an Hsp70 terminator flanks the 3' end of the Scer\GAL4 coding region. (Lukacsovich and Asztalos, 1999.7.15)
Phenotypic class
Phenotype manifest in
Other information

Allele	Tn\neoR^Hsp70Bb.PS
Reference(s)	(Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data	Construct: Tn\neoR gene lacking the bacterial promoter but driven by 465bp Xba Xmn fragment of the Hsp70 heat shock promoter and 206 nucleotides of untranslated leader sequence. (Steller and Pirrotta, 1985)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mGT
Reference(s)	(Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data	Removal of the 3' untranslated region (including the poly(A) signal sequence). An artificial splice donor site follows the w sequence, allowing the production of a chimeric mRNA composed of the w sequence and the exonic sequence of a host gene 3' to the vector integration site when the P{GT1} or P{GTD-b} vector is inserted into the genome. (Lukacsovich et al., 2001) Construct: The 3' UTR of w^+mC has been removed and replaced with a synthetic artificial consensus splicing donor site. The result is the w^+mC coding region with promoter and stop codon, but no poly-A site. (Lukacsovich and Asztalos, 1999.7.15)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{GT1} (Lukacsovich and Asztalos, 1999.7.15)
descendant(s)	P{3'GT1} (Lee et al., 2009) P{?'GT1} (Lee et al., 2009) P{?GT1} (Dasgupta et al., 2009) P{EGFP.GT} (Jankovics et al., 2002)
Comments
	Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html (Bellen et al., 2004) construct_comment: Insertion in proper orientation within a gene allows white expression at high levels (due to increased stability of resulting fusion protein containing polyA site); allows visual selection of candidate insertions. (Lukacsovich et al., 2001) construct_comment: Designed to allow detection of insertion (if in proper orientation) within the transcribed portion of a gene; results in transcription of Scer\GAL4 under control of regulatory and promoter elements of the endogenous gene. (Lukacsovich and Asztalos, 1999.7.15) report comment: Note: opposite orientation in sequence entry AB028139 \| g4995951 \| 03 Jun 1999. (Lukacsovich and Asztalos, 1999.7.15)
Stocks Listed in FlyBase ( 545 )
Bloomington	12404 w^*; P{GT1}BG00081 12399 w¹¹¹⁸ P{GT1}BG00040 12412 w¹¹¹⁸ P{GT1}BG00310 12415 w¹¹¹⁸ P{GT1}BG00387 12423 P{GT1}BG00541 w¹¹¹⁸ 12453 w¹¹¹⁸ P{GT1}BG01082 12468 w¹¹¹⁸ P{GT1}BG01319 12471 w¹¹¹⁸ P{GT1}BG01461 12472 w¹¹¹⁸ P{GT1}BG01463 12478 w¹¹¹⁸ P{GT1}BG00175 12494 w¹¹¹⁸ P{GT1}BG00574
Kyoto	109055 w^*; P{GT1}7-65
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External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	BG (Bellen et al., 2004) dual-tagging gene trap (FlyBase, 1996-) P{GT1} (FlyBase, 1996-, Parks et al., 2004, Gene Disruption Project members, 2001-, Lukacsovich et al., 2001, Bellen et al., 2004, Lukacsovich, 1999.5.31)
Secondary FlyBase IDs

References ( 25 )

Generate a list of	All references relating to this transgenic transposon ( 25 )

List References by type	Research paper ( 20 ) Personal communication to FlyBase ( 3 ) FlyBase analysis ( 1 ) DNA/RNA sequence record ( 1 )
Recent research papers ( 3 )
	Tunstall et al., 2012, PLoS ONE 7(4): e35641 A Screen for Genes Expressed in the Olfactory Organs of Drosophila melanogaster Identifies Genes Involved in Olfactory Behaviour. [FBrf0218110] Xu et al., 2012, PLoS Genet. 8(1): e1002478 Insulin Signaling Regulates Fatty Acid Catabolism at the Level of CoA Activation. [FBrf0217254] Sakaidani et al., 2011, Nat. Commun. 2: 583 O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions. [FBrf0217928] Show all research papers ...