A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Recombinant construct P{GT1}

General Information
Symbol P{GT1} FlyBase ID FBtp0002720
Feature type transgenic_transposon
Size 8.447 Expression data
Associated insertions 1013 available
Molecular map
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Description
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FB2013_03
Alleles
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Description & Uses
Species synthetic construct
Location-dependent
role
Description
CV term
Qualifiers & info
Reference
Uses
CV term
Qualifiers & info
Reference
genomic insertion screen
Cloning Sites
Location
Restriction sites
Reference
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Sequence (FB)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
(bases 1-8447)
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Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
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CV term
Qualifiers & info
Reference
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Allele Scer\GAL4GT1
Reference(s) (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data
Scer\GAL4 coding region with a poly(A) signal sequence but lacking a promoter. The coding sequence is preceeded by an artificial splice acceptor site, which allows the Scer\GAL4 sequence to be transcribed as a fusion mRNA with an upstream exon sequence when the P{GT1} element is inserted into the genome. A "stop-start" sequence is placed between the splice acceptor site and the Scer\GAL4 gene which ensures that the Scer\GAL4 open reading frame is maintained in any integration event.
Construct: The Scer\GAL4 wild type coding sequences start at the ATG of the coding sequence, with 5' UTR removed. The coding sequence thus has no promoter. A synthetic oligonucleotide containing a "stop-start" signal has been placed immediately 5' of the coding region. This sequence has consecutive overlapping transcriptional stop and start codons with which otherwise variable reading frames can be set into the proper reading frame for Scer\GAL4. The frameshift sequence was designed to be similar to the functional kozak start site, to allow translation to initiate from the Scer\GAL4 ATG if an untranslated exon is spliced to the Scer\GAL4 mRNA. An artificial splice acceptor site was engineered to be 5' of the "stop-start" signal, and an Hsp70 terminator flanks the 3' end of the Scer\GAL4 coding region.
Phenotypic class
Phenotype manifest in
Other information
Allele Tn\neoRHsp70Bb.PS
Reference(s) (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data
Construct: Tn\neoR gene lacking the bacterial promoter but driven by 465bp Xba Xmn fragment of the Hsp70 heat shock promoter and 206 nucleotides of untranslated leader sequence.
Phenotypic class
Phenotype manifest in
Other information
Allele w+mGT
Reference(s) (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data
Removal of the 3' untranslated region (including the poly(A) signal sequence). An artificial splice donor site follows the w sequence, allowing the production of a chimeric mRNA composed of the w sequence and the exonic sequence of a host gene 3' to the vector integration site when the P{GT1} or P{GTD-b} vector is inserted into the genome.
Construct: The 3' UTR of w+mC has been removed and replaced with a synthetic artificial consensus splicing donor site. The result is the w+mC coding region with promoter and stop codon, but no poly-A site.
Phenotypic class
Phenotype manifest in
Other information
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Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects expression of
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progenitor(s)
descendant(s)
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Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html
construct_comment: Insertion in proper orientation within a gene allows white expression at high levels (due to increased stability of resulting fusion protein containing polyA site); allows visual selection of candidate insertions.
construct_comment: Designed to allow detection of insertion (if in proper orientation) within the transcribed portion of a gene; results in transcription of Scer\GAL4 under control of regulatory and promoter elements of the endogenous gene.
report comment: Note: opposite orientation in sequence entry AB028139 | g4995951 | 03 Jun 1999.
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Bloomington
Kyoto
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Reported As
Symbol Synonym
dual-tagging gene trap
Secondary FlyBase IDs
hide References ( 25 )
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hide Recent research papers ( 3 )
Tunstall et al., 2012, PLoS ONE 7(4): e35641
A Screen for Genes Expressed in the Olfactory Organs of Drosophila melanogaster Identifies Genes Involved in Olfactory Behaviour. [FBrf0218110]
Xu et al., 2012, PLoS Genet. 8(1): e1002478
Insulin Signaling Regulates Fatty Acid Catabolism at the Level of CoA Activation. [FBrf0217254]
Sakaidani et al., 2011, Nat. Commun. 2: 583
O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions. [FBrf0217928]