Recombinant construct P{GT1}

General Information
Symbol	P{GT1}	FlyBase ID	FBtp0002720
Feature type	transgenic_transposon	Created / Updated	1999-09-13/2001-12-17

Size	8.447	Expression data
Location-dependent role		Associated insertions	836 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001, Parks et al., 2004, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference gene trap P-element (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001) binary system (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent AB028139 (bases 1-8447)
Segments & Size
Total Size	8.447 (Lukacsovich and Asztalos, 1999.7.15)
Left end	P5\' (Lukacsovich and Asztalos, 1999.7.15)
Right end	P3\' (Lukacsovich and Asztalos, 1999.7.15)

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference gene trap (Lukacsovich and Asztalos, 1999.7.15) gene trap (Lukacsovich and Asztalos, 1999.7.15) selectable marker (Lukacsovich and Asztalos, 1999.7.15) selectable marker (Lukacsovich and Asztalos, 1999.7.15) reporter gene (Lukacsovich and Asztalos, 1999.7.15)
Component Alleles
Tn\neoR^Hsp70Bb.PS
Reference(s)	(Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data	Construct: Tn\neoR gene lacking the bacterial promoter but driven by 465bp Xba Xmn fragment of the Hsp70 heat shock promoter and 206 nucleotides of untranslated leader sequence. (Steller and Pirrotta, 1985)
Phenotypic class
Phenotype manifest in
Other information
w^+mGT
Reference(s)	(Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data	Construct: The 3' UTR of w^+mC has been removed and replaced with a synthetic artificial consensus splicing donor site. The result is the w^+mC coding region with promoter and stop codon, but no poly-A site. (Lukacsovich and Asztalos, 1999.7.15) Removal of the 3' untranslated region (including the poly(A) signal sequence). An artificial splice donor site follows the w sequence, allowing the production of a chimeric mRNA composed of the w sequence and the exonic sequence of a host gene 3' to the vector integration site when the P{GT1} or P{GTD-b} vector is inserted into the genome. (Lukacsovich et al., 2001)
Phenotypic class
Phenotype manifest in
Other information
Scer\GAL4^GT1
Reference(s)	(Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data	Construct: The Scer\GAL4 wild type coding sequences start at the ATG of the coding sequence, with 5' UTR removed. The coding sequence thus has no promoter. A synthetic oligonucleotide containing a "stop-start" signal has been placed immediately 5' of the coding region. This sequence has consecutive overlapping transcriptional stop and start codons with which otherwise variable reading frames can be set into the proper reading frame for Scer\GAL4. The frameshift sequence was designed to be similar to the functional kozak start site, to allow translation to initiate from the Scer\GAL4 ATG if an untranslated exon is spliced to the Scer\GAL4 mRNA. An artificial splice acceptor site was engineered to be 5' of the "stop-start" signal, and an Hsp70 terminator flanks the 3' end of the Scer\GAL4 coding region. (Lukacsovich and Asztalos, 1999.7.15) Scer\GAL4 coding region with a poly(A) signal sequence but lacking a promoter. The coding sequence is preceeded by an artificial splice acceptor site, which allows the Scer\GAL4 sequence to be transcribed as a fusion mRNA with an upstream exon sequence when the P{GT1} element is inserted into the genome. A "stop-start" sequence is placed between the splice acceptor site and the Scer\GAL4 gene which ensures that the Scer\GAL4 open reading frame is maintained in any integration event. (Lukacsovich et al., 2001)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{GT1} (Lukacsovich and Asztalos, 1999.7.15)
descendant(s)	P{EGFP.GT} (Jankovics et al., 2002)
Comments
	construct_comment: Insertion in proper orientation within a gene allows white expression at high levels (due to increased stability of resulting fusion protein containing polyA site); allows visual selection of candidate insertions. (Lukacsovich et al., 2001) construct_comment: Designed to allow detection of insertion (if in proper orientation) within the transcribed portion of a gene; results in transcription of Scer\GAL4 under control of regulatory and promoter elements of the endogenous gene. (Lukacsovich and Asztalos, 1999.7.15) report comment: Note: opposite orientation in sequence entry AB028139 \| g4995951 \| 03 Jun 1999. (Lukacsovich and Asztalos, 1999.7.15)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	dual-tagging gene trap (FlyBase, 1996-) P{@w^+mGT1@ @Tn\neoR^Hsp70Bb.PS@ @Scer\GAL4^GT1@=@GT1@} (FlyBase, 1996-) P{GT1} (FlyBase, 1996-, Parks et al., 2004, Gene Disruption Project members, 2001-, Lukacsovich et al., 2001, Bellen et al., 2004, Lukacsovich, 1999.5.31)
Secondary FlyBase IDs

References ( 13 )
Research paper	Jung et al., 2006, Curr. Biol. 16(12): 1224--1231 Polychaetoid/ZO-1 is required for cell specification and rearrangement during Drosophila tracheal morphogenesis. [FBrf0192782] Sambandan et al., 2006, Genetics 174(3): 1349--1363 Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster. [FBrf0192106] Zhai et al., 2006, PLoS Biol. 4(12): Drosophila NMNAT maintains neural integrity independent of its NAD synthesis activity. [FBrf0192124] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Parks et al., 2004, Nature Genetics 36(3): 288--292 Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. [FBrf0175003] Jankovics et al., 2002, Curr. Biol. 12(23): 2060--2065 MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring. [FBrf0155622] Lukacsovich et al., 2001, Genetics 157(2): 727--742 Dual-tagging Gene Trap of novel genes in Drosophila melanogaster. [FBrf0132344] Steller and Pirrotta, 1985, EMBO J. 4(1): 167--171 A transposable P vector that confers selectable G418 resistance to Drosophila larvae. [FBrf0042419]
Personal communication to FlyBase	BDGP TI submission, 2008.1.15, batch770_take2.xml batch770_take2.xml [FBrf0202437] Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster. Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]
DNA/RNA sequence record	Lukacsovich, 1999.5.31, GenBank/EMBL/DDBJ: AB028139 Drosophila melanogaster genes for Gal4, neomycine phosphotransferase and white protein, complete cds. [FBrf0117594]