A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Recombinant construct P{GT1}

General Information
Symbol P{GT1} FlyBase ID FBtp0002720
Feature type transgenic_transposon Created / Updated 1999-09-13/2001-12-17
Size 8.447 Expression data
Location-dependent role Associated insertions 836 available
Molecular map
hide Description & Uses
Species synthetic construct
Description
CV term
Qualifiers & info
Reference
Uses
CV term
Qualifiers & info
Reference
genomic insertion screen
Cloning Sites
Location
Restriction sites
Reference
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Sequence (FB)
Sequence
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
(bases 1-8447)
hide Segments & Size
Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
hide Features
CV term
Qualifiers & info
Reference
hide Component Alleles
hideTn\neoRHsp70Bb.PS
Reference(s) (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data
Construct: Tn\neoR gene lacking the bacterial promoter but driven by 465bp Xba Xmn fragment of the Hsp70 heat shock promoter and 206 nucleotides of untranslated leader sequence.
Phenotypic class
Phenotype manifest in
Other information
hidew+mGT
Reference(s) (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data
Construct: The 3' UTR of w+mC has been removed and replaced with a synthetic artificial consensus splicing donor site. The result is the w+mC coding region with promoter and stop codon, but no poly-A site.
Removal of the 3' untranslated region (including the poly(A) signal sequence). An artificial splice donor site follows the w sequence, allowing the production of a chimeric mRNA composed of the w sequence and the exonic sequence of a host gene 3' to the vector integration site when the P{GT1} or P{GTD-b} vector is inserted into the genome.
Phenotypic class
Phenotype manifest in
Other information
hideScer\GAL4GT1
Reference(s) (Lukacsovich and Asztalos, 1999.7.15, Lukacsovich et al., 2001)
Molecular data
Construct: The Scer\GAL4 wild type coding sequences start at the ATG of the coding sequence, with 5' UTR removed. The coding sequence thus has no promoter. A synthetic oligonucleotide containing a "stop-start" signal has been placed immediately 5' of the coding region. This sequence has consecutive overlapping transcriptional stop and start codons with which otherwise variable reading frames can be set into the proper reading frame for Scer\GAL4. The frameshift sequence was designed to be similar to the functional kozak start site, to allow translation to initiate from the Scer\GAL4 ATG if an untranslated exon is spliced to the Scer\GAL4 mRNA. An artificial splice acceptor site was engineered to be 5' of the "stop-start" signal, and an Hsp70 terminator flanks the 3' end of the Scer\GAL4 coding region.
Scer\GAL4 coding region with a poly(A) signal sequence but lacking a promoter. The coding sequence is preceeded by an artificial splice acceptor site, which allows the Scer\GAL4 sequence to be transcribed as a fusion mRNA with an upstream exon sequence when the P{GT1} element is inserted into the genome. A "stop-start" sequence is placed between the splice acceptor site and the Scer\GAL4 gene which ensures that the Scer\GAL4 open reading frame is maintained in any integration event.
Phenotypic class
Phenotype manifest in
Other information
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Reporter Expression
Stage
Tissue/Position
Reference
Additional Information
Statement
Reference
Assay mode
Marker for
Reflects expression of
hide Progenitors & Descendants
progenitor(s)
descendant(s)
hide Comments
construct_comment: Insertion in proper orientation within a gene allows white expression at high levels (due to increased stability of resulting fusion protein containing polyA site); allows visual selection of candidate insertions.
construct_comment: Designed to allow detection of insertion (if in proper orientation) within the transcribed portion of a gene; results in transcription of Scer\GAL4 under control of regulatory and promoter elements of the endogenous gene.
report comment: Note: opposite orientation in sequence entry AB028139 | g4995951 | 03 Jun 1999.
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
dual-tagging gene trap
P{@w+mGT1@ @Tn\neoRHsp70Bb.PS@ @Scer\GAL4GT1@=@GT1@}
Secondary FlyBase IDs
    hide References ( 13 )
    Research paper
    Jung et al., 2006, Curr. Biol. 16(12): 1224--1231
    Polychaetoid/ZO-1 is required for cell specification and rearrangement during Drosophila tracheal morphogenesis. [FBrf0192782]
    Sambandan et al., 2006, Genetics 174(3): 1349--1363
    Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster. [FBrf0192106]
    Zhai et al., 2006, PLoS Biol. 4(12):
    Drosophila NMNAT maintains neural integrity independent of its NAD synthesis activity. [FBrf0192124]
    Bellen et al., 2004, Genetics 167(2): 761--781
    The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
    Parks et al., 2004, Nature Genetics 36(3): 288--292
    Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. [FBrf0175003]
    Jankovics et al., 2002, Curr. Biol. 12(23): 2060--2065
    MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring. [FBrf0155622]
    Lukacsovich et al., 2001, Genetics 157(2): 727--742
    Dual-tagging Gene Trap of novel genes in Drosophila melanogaster. [FBrf0132344]
    Steller and Pirrotta, 1985, EMBO J. 4(1): 167--171
    A transposable P vector that confers selectable G418 resistance to Drosophila larvae. [FBrf0042419]
    Personal communication to FlyBase
    BDGP TI submission, 2008.1.15, batch770_take2.xml
    batch770_take2.xml [FBrf0202437]
    Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster.
    Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
    FlyBase analysis
    FlyBase, 1996-, FlyBase inference based on genome sequence analysis.
    FlyBase inference based on genome sequence analysis. [FBrf0104946]
    Computer file
    Gene Disruption Project members, 2001-, [title not yet available]
    [title not yet available] [FBrf0132177]
    DNA/RNA sequence record
    Lukacsovich, 1999.5.31, GenBank/EMBL/DDBJ: AB028139
    Drosophila melanogaster genes for Gal4, neomycine phosphotransferase and white protein, complete cds. [FBrf0117594]