Recombinant construct P{GMR-HAFAS}

General Information
Symbol	P{GMR-HAFAS}	FlyBase ID	FBtp0002760
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Hay et al., 1997)
Uses	CV term Qualifiers & info Reference characterization apoptosis (Hay et al., 1997) GMR (Hay et al., 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Hay et al., 1997) chimeric protein (Hay et al., 1997) molecular tag (Hay et al., 1997) selectable marker (Hay et al., 1997)
Component Alleles
Allele	Hsap\FAS^{GMR.T:Ivir\haemagglutinin}
Reference(s)	(Hay et al., 1997)
Molecular data	Extracellular domain of T:Ivir\haemagglutinin (157 amino acids containing 3 Cys rich subdomains) fused to the transmembrane and intracellular domain of Hsap\TNFRSF6. Expression is driven by GMR, Hsp70 TATA box and 200 bases of 5' untranslated region and Hsp70 3' untranslated region. (Hay et al., 1997)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Hay et al., 1997)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{GMR} (Hay et al., 1997)
descendant(s)
Comments
	construct_comment: Progenitors are those of the plasmid, pP{GMR-HAFAS}. (Hay et al., 1997) report comment: Ivir\HA (influenza hemagglutinin) extracellular domain known to form trimers; expected to induce multimerization of the chimeric protein. (Hay et al., 1997)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{GMR-HAFAS} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Hay et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94(10): 5195--5200 P element insertion-dependent gene activation in the Drosophila eye. [FBrf0093501]