Recombinant construct P{Dipt2.2-lacZ}

General Information
Symbol	P{Dipt2.2-lacZ}	FlyBase ID	FBtp0004148
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Reichhart et al., 1992)
Uses	CV term Qualifiers & info Reference reporter construct Dpt (Reichhart et al., 1992)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Reichhart et al., 1992) selectable marker (Reichhart et al., 1992, Lemaitre et al., 1995, Lemaitre et al., 1995, Meister et al., 1994)
Component Alleles
Allele	Ecol\lacZ^Dpt.PR
Reference(s)	(Reichhart et al., 1992, Gottar et al., 2002, Choe et al., 2002, Vodovar et al., 2005, Michel et al., 2001, Wu and Anderson, 1998, Seroude et al., 2002, Wu et al., 2001, Lemaitre et al., 1997, Georgel et al., 2001, Ha et al., 2005, Kim et al., 2006, Tulin and Spradling, 2003, Foley and O'Farrell, 2003, Kappler et al., 1993, Takehana et al., 2002, Foley and O'Farrell, 2004, Lemaitre et al., 1995, Pili-Floury et al., 2004, De Gregorio et al., 2002, Benassi et al., 2000, Meister et al., 1994, Nappi et al., 2000, Jung et al., 2001, Tzou et al., 2000, Basset et al., 2000, Manfruelli et al., 1999, Chang et al., 2004, Takehana et al., 2004, Braun et al., 1998, Stein et al., 1998, Lemaitre et al., 1995, Tingvall et al., 2001, Vidal et al., 2001, Meister and Richards, 1996, Nicolas et al., 1996, Wu and Anderson, 1997, Cha et al., 2003, Rutschmann et al., 2000, Gendrin et al., 2009, Paredes et al., 2011, Maillet et al., 2008, Lhocine et al., 2008, Buchon et al., 2009, Libert et al., 2006, Kikuchi et al., 2011, Thevenon et al., 2009, Bischoff et al., 2006, Chakrabarti et al., 2012, Hashimoto et al., 2009)
Molecular data	2.2kb Dpt upstream sequences drive the expression of Ecol\lacZ. (Lemaitre et al., 1995) 2.2kb of Dpt upstream sequences fused to Ecol\lacZ coding region. (Lemaitre et al., 1995) 2.2kb promoter fragment from Dpt drives expression of Ecol\lacZ. (Kappler et al., 1993) Construct: Ecol\lacZ expression is driven by 2.2kb Dpt promoter region. (Reichhart et al., 1992)
Phenotypic class
Phenotype manifest in
Other information

Allele	ry^+t7.2
Reference(s)	(Meister et al., 1994, Lemaitre et al., 1995, Reichhart et al., 1992, Lemaitre et al., 1995)
Molecular data	Construct: 7.2kb HindIII fragment that contains ry⁺ function. (Rubin and Spradling, 1983)
Phenotypic class	wild-type (Rubin and Spradling, 1983)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference Reporter gene expression is induced inresponse to injection of complete Freund's adjuvant in late third instarlarvae (96-120hr). Younger animals do not express the reporter gene inresponse to the injection. From pupariation on, there is a gradualdecrease in the response to the challenge. In response to live bacteria, afaint response is seen in second instar larvae and a full response is seenin adults. (Reichhart et al., 1992)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 5 )
Bloomington	30916 P{Dipt2.2-lacZ}3, ird5¹ ca¹/TM6B, Tb¹ 31773 w^; P{UAS-PGRP-LC.a}2; P{Dipt2.2-lacZ}3, PGRP-LC¹ ca¹/TM6B 30918 w^; P{UAS-PGRP-LC.x}2; P{Dipt2.2-lacZ}3, PGRP-LC¹ ca¹/TM6B, Tb¹ 36558 P{Dipt2.2-lacZ}1, P{Drs-GFP.JM804}1, y¹ w^; Dif² cn¹ bw¹ 36559 P{Dipt2.2-lacZ}1, P{Drs-GFP.JM804}1, y¹ w^; Dif¹ cn¹ bw¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Dep-lacZ (Libert et al., 2006) Dipt2.2 (Tzou et al., 2000) Dipt2.2-LacZ (Benassi et al., 2000) Dipt2.2-LacZ:1 (Nappi et al., 2000) diptericin 2.2-lacZ (Tingvall et al., 2001) Diptericine-LacZ (Tulin and Spradling, 2003) diptericin-lacZ (Ha et al., 2005, Kim et al., 2006) Diptericin-lacZ (Chang et al., 2004, Pili-Floury et al., 2004, Vidal et al., 2001, Gendrin et al., 2009, Libert et al., 2006) diptericin-LacZ (Cha et al., 2003, Maillet et al., 2008) Dipt-lacZ (Foley and O'Farrell, 2004, Foley and O'Farrell, 2003, Tzou et al., 2000, Thevenon et al., 2009, Maillet et al., 2008) dipt-lacZ (Seroude et al., 2002, Wu et al., 2001, Basset et al., 2000, Manfruelli et al., 1999, Kim et al., 2006, Hashimoto et al., 2009) dipt-LacZ (Maillet et al., 2008) Dipt-LacZ (Maillet et al., 2008) DiptlacZ (Bischoff et al., 2006) Dpt::LacZ (Paredes et al., 2011) Dpt-lacZ (Vodovar et al., 2005, Takehana et al., 2004, Takehana et al., 2002, Choe et al., 2002, Buchon et al., 2009, Kikuchi et al., 2011, Chakrabarti et al., 2012) Dpt-LacZ (Gottar et al., 2002, Lhocine et al., 2008) dpt-lacZ (Michel et al., 2001) P{Dipt2.2-lacZ} (FlyBase, 1996-) Pdipt-lacZ (Georgel et al., 2001) pDipt-LacZ (Jung et al., 2001) pdipt-lacZ (Rutschmann et al., 2000)
Secondary FlyBase IDs

References ( 52 )

Generate a list of	All references relating to this transgenic transposon ( 52 )

List References by type	Research paper ( 47 ) Supplementary material ( 1 ) Review ( 1 ) Personal communication to FlyBase ( 2 ) Letter ( 1 )
Recent research papers ( 3 )
	Chakrabarti et al., 2012, Cell Host Microbe 12(1): 60--70 Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. [FBrf0218984] Kikuchi et al., 2011, Eur. J. Med. Chem. 46(4): 1263--1273 Synthesis and innate immunosuppressive effect of 1,2-cyclopentanediol derivatives. [FBrf0213182] Paredes et al., 2011, Immunity 35(5): 770--779 Negative Regulation by Amidase PGRPs Shapes the Drosophila Antibacterial Response and Protects the Fly from Innocuous Infection. [FBrf0216727] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...