Recombinant construct P{lacZ^{zen/eve.600bpVRE}}

General Information
Symbol	P{lacZ^{zen/eve.600bpVRE}}	FlyBase ID	FBtp0004696
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Jiang et al., 1992)
Uses	CV term Qualifiers & info Reference reporter construct eve (Cai and Levine, 1995, Jiang et al., 1993, Jiang et al., 1992, Cai et al., 1996) zen (Cai and Levine, 1995, Jiang et al., 1993, Jiang et al., 1992, Cai et al., 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Jiang et al., 1992) selectable marker (Cai and Levine, 1995)
Component Alleles
Allele	Ecol\lacZ^{zen:eve.VR:MSE}
Reference(s)	(Jiang et al., 1992, Jiang et al., 1993, Jimenez et al., 2000, Cai and Levine, 1995, Cai et al., 1996, ten Bosch et al., 2006)
Molecular data	Construct: The complete 600bp zen ventral repression element (VRE) is fused upstream of a minimal 480bp eve stripe 2 enhancer (MSE), which drives expression of an eve-Ecol\lacZ fusion gene. (Cai et al., 1996) 600bp zen ventral repression element (VRE) is fused upstream of eve MSE. (Cai and Levine, 1995) 600bp XbaI-HindIII fragment carrying the complete zen ventral repression element (VRE) from position -1.6 to -1.0 is fused upstream of eve MSE. (Jiang et al., 1993) Ecol\lacZ gene expression is driven by zen-eve promoter region fusion. (Jiang et al., 1992)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Cai and Levine, 1995)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic cycle 14A organism \| dorsal (Jiang et al., 1992) embryonic cycle 14B organism \| dorsal & striped (Jiang et al., 1992)
Additional Information
Statement Reference In stage 5 embryo, the ventral portion of the eve second stripe pattern is repressed; in addition, there is dorsal expression outside the eve second stripe. This results, during embryonic cycle 14A, in dorsal expression from the region of eve stripe 2 and more posterior; and during embryonic cycle 14B, in high levels of expression in the dorsal half of the eve stripe 2 region, with lower levels in more posterior dorsal regions. (Jiang et al., 1992)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	600VRE (ten Bosch et al., 2006) P{lacZ^{zen/eve.600bpVRE}} (FlyBase, 1996-) St.2-lacZ-VRE 600 (Jimenez et al., 2000)
Secondary FlyBase IDs

References ( 6 )
Research paper	ten Bosch et al., 2006, Development 133(10): 1967--1977 The TAGteam DNA motif controls the timing of Drosophila pre-blastoderm transcription. [FBrf0190276] Jimenez et al., 2000, Genes Dev. 14(2): 224--231 Relief of gene repression by torso RTK signaling: role of capicua in Drosophila terminal and dorsoventral patterning. [FBrf0125241] Cai et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 93(18): 9309--9314 Long-range repression in the Drosophila embryo. [FBrf0090481] Cai and Levine, 1995, Nature 376(6540): 533--536 Modulation of enhancer-promoter interactions by insulators in the Drosophila embryo. [FBrf0083065] Jiang et al., 1993, EMBO J. 12(8): 3201--3209 Conversion of a dorsal-dependent silencer into an enhancer: evidence for dorsal corepressors. [FBrf0064528] Jiang et al., 1992, EMBO J. 11: 3147--3154 Individual dorsal morphogen binding sites mediate activation and repression in the Drosophila embryo. [FBrf0055953]

Recombinant construct P{lacZzen/eve.600bpVRE}

Recombinant construct P{lacZ^{zen/eve.600bpVRE}}