Recombinant construct P{R8}

General Information
Symbol	P{R8}	FlyBase ID	FBtp0005073
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Harrison et al., 1994)
Uses	CV term Qualifiers & info Reference characterization Ras64B (Harrison et al., 1994) RfC40 (Harrison et al., 1994) Rop (Harrison et al., 1994) rescue construct Ras64B (Harrison et al., 1994) RfC40 (Harrison et al., 1994) Rop (Harrison et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference complete rescue (Harrison et al., 1994) complete rescue (Harrison et al., 1994) complete rescue (Harrison et al., 1994) selectable marker (Harrison et al., 1994, Harrison et al., 1995)
Component Alleles
Allele	Ras64B^+t10.2
Reference(s)	(Harrison et al., 1994, Harrison et al., 1995)
Molecular data	Construct: 10.2kb XbaI-SpeI genomic fragment including the Rop, Ras64B and RfC40 transcription units. (Harrison et al., 1995) 10.2kb SpeI-XbaI fragment containing RfC40, Rop and Ras64B. (Harrison et al., 1994)
Phenotypic class
Phenotype manifest in
Other information

Allele	RfC4^+t10.2
Reference(s)	(Harrison et al., 1994, Harrison et al., 1995)
Molecular data	Construct: 10.2kb XbaI-SpeI genomic fragment including the Rop, Ras64B and RfC40 transcription units. (Harrison et al., 1995) 10.2kb SpeI-XbaI fragment containing RfC40, Rop and Ras64B. (Harrison et al., 1994)
Phenotypic class	wild-type (Harrison et al., 1995)
Phenotype manifest in
Other information

Allele	Rop^+t10.2
Reference(s)	(Harrison et al., 1994, Harrison et al., 1995)
Molecular data	Construct: 10.2kb XbaI-SpeI genomic fragment including the Rop, Ras64B and RfC40 transcription units. (Harrison et al., 1995) 10.2kb SpeI-XbaI fragment containing RfC40, Rop and Ras64B. (Harrison et al., 1994)
Phenotypic class	wild-type (Harrison et al., 1995, Harrison et al., 1994)
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Harrison et al., 1995, Harrison et al., 1994)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{R8} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 2 )
Research paper	Harrison et al., 1995, Genetics 139(4): 1701--1709 A genetic analysis of the 63E-64A genomic region of Drosophila melanogaster: Identification of mutations in a replication factor C subunit. [FBrf0080079] Harrison et al., 1994, Neuron 13(3): 555--566 Mutations in the Drosophila Rop gene suggest a function in general secretion and synaptic transmission. [FBrf0077006]