Recombinant construct P{hsp70-NSF}

General Information
Symbol	P{hsp70-NSF}	FlyBase ID	FBtp0005150
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Pallanck et al., 1995)
Uses	CV term Qualifiers & info Reference characterization comt (Pallanck et al., 1995) heat-shock construct comt (Pallanck et al., 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference inducible promoter (Pallanck et al., 1995)
Component Alleles
Allele	comt^hs.PP
Reference(s)	(Pallanck et al., 1995, Dunne et al., 2002, Kondylis et al., 2001, Kawasaki et al., 1998, Tolar and Pallanck, 1998, Golby et al., 2001, Sanyal and Krishnan, 2001)
Molecular data	Construct: Full length comt cDNA under the control of an Hsp70 promoter. (Pallanck et al., 1995)
Phenotypic class	wild-type (Kawasaki et al., 1998, Kondylis et al., 2001)
Phenotype manifest in	Golgi apparatus (Dunne et al., 2002) Golgi stack \| ectopic (Dunne et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	HSN (Dunne et al., 2002, Kondylis et al., 2001) hs-NSF1 (Sanyal and Krishnan, 2001) hsp70-dNsf1 (Golby et al., 2001) P{hsp70-NSF} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Dunne et al., 2002, Dev. Biol. 245(1): 172--186 Ecdysone triggers the expression of golgi genes in Drosophila imaginal discs via Broad-Complex. [FBrf0146969] Golby et al., 2001, Genetics 158(1): 265--278 Partitioning of N-ethylmaleimide-sensitive fusion (NSF) protein function in Drosophila melanogaster. Dnsf1 is required in the nervous system, and dnsf2 is required in mesoderm. [FBrf0135807] Kondylis et al., 2001, Mol. Biol. Cell 12(8): 2308--2327 Biogenesis of Golgi stacks in imaginal discs of Drosophila melanogaster. [FBrf0138491] Sanyal and Krishnan, 2001, Neuroreport 12(7): 1363--1366 Lethal comatose mutation in Drosophila reveals possible role for NSF in neurogenesis. [FBrf0137072] Kawasaki et al., 1998, J. Neurosci. 18(24): 10241--10249 Synaptic physiology and ultrastructure in comatose mutants define an in vivo role for NSF in neurotransmitter release. [FBrf0105979] Tolar and Pallanck, 1998, J. Neurosci. 18(24): 10250--10256 NSF function in neurotransmitter release involves rearrangement of the SNARE complex downstream of synaptic vesicle docking. [FBrf0105980]
Letter	Pallanck et al., 1995, Nature 376(6535): 25 A Drosophila NSF mutant. [FBrf0082439]