Recombinant construct P{p7.0labTh}

General Information
Symbol	P{p7.0labTh}	FlyBase ID	FBtp0005681
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Chouinard and Kaufman, 1991)
Uses	CV term Qualifiers & info Reference reporter construct lab (Chouinard and Kaufman, 1991)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Chouinard and Kaufman, 1991) selectable marker (Chouinard and Kaufman, 1991)
Component Alleles
Allele	Ecol\lacZ^lab.7.0.Th
Reference(s)	(Chouinard and Kaufman, 1991, Nagao et al., 2000, Miller et al., 2001)
Molecular data	Contains 6kb of genomic sequences (containing the amg enhancer element), in addition to 0.99kb of sequences from the lab transcription unit which encode the N-terminal 252 amino acids of lab. (Miller et al., 2001) Construct: Contains approximately 6.0kb upstream lab sequences, the lab transcription start site, and 995bp transcribed lab sequence (which include sequence encoding the first 252 amino acids of lab), driving expression of Ecol\lacZ. (Chouinard and Kaufman, 1991)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Chouinard and Kaufman, 1991)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	lab-lacZ (Nagao et al., 2000) P{p7.0labTh} (FlyBase, 1996-) P{w^+mW.hs,7.0lab::LacZ} (Miller et al., 2001)
Secondary FlyBase IDs

References ( 3 )
Research paper	Miller et al., 2001, Mech. Dev. 102(1-2): 17--32 Homeotic Complex (Hox) gene regulation and homeosis in the mesoderm of the Drosophila melanogaster embryo: the roles of signal transduction and cell autonomous regulation. [FBrf0135875] Nagao et al., 2000, Dev. Genes Evol. 210(6): 289--299 Patterning defects in the primary axonal scaffolds caused by the mutations of the extradenticle and homothorax genes in the embryonic Drosophila brain. [FBrf0128584] Chouinard and Kaufman, 1991, Development 113(4): 1267--1280 Control of expression of the homeotic labial (lab) locus of Drosophila melanogaster: evidence for both positive and negative autogenous regulation. [FBrf0053767]