Recombinant construct P{eve-lacZ.C}

General Information
Symbol	P{eve-lacZ.C}	FlyBase ID	FBtp0006046
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Yan et al., 1996)
Uses	CV term Qualifiers & info Reference reporter construct eve (Yan et al., 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Yan et al., 1996) selectable marker (Yan et al., 1996, Small et al., 1996)
Component Alleles
Allele	Ecol\lacZ^eve.C
Reference(s)	(Yan et al., 1996, Andrioli et al., 2002, Struffi et al., 2004, Keller et al., 2000, Jennings et al., 2004, Small et al., 1996, Garcia and Stathopoulos, 2011, Clyde et al., 2003)
Molecular data	500bp region extending from position -3.8kb to -2.3kb, the full length eve stripe 3+7 enhancer, mediates expression of a minimal eve-Ecol\lacZ fusion (eve TATA box and basal promoter, 100bp eve 5' untranslated leader and the first 22 codons of the protein coding sequence fused to codon 5 of Ecol\lacZ). (Small et al., 1996) Construct: Ecol\lacZ expression is driven by an eve stripe 3 enhancer, extending between 3.4kb and 3.9kb upstream of the eve transcription start site and the eve minimal promoter. (Yan et al., 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Small et al., 1996, Yan et al., 1996)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 5 organism \| striped (Small et al., 1996) parasegment 5 (Small et al., 1996) parasegment 13 (Small et al., 1996) embryonic stage 5 organism \| striped (Yan et al., 1996) Comment:eve stripes 3 and 7
Additional Information
Statement Reference Reflects expression of eve stripes 3 and 7. (Small et al., 1996) In embryo, expressed stripes 3 and 7, a subset of the normal 7 eve stripes; expression in stripe 3 stronger than that in stripe 7. A small dorsal and anterior patch of expression is also observed; this is shown to be due to vector sequences in the P-transposon construct. (Small et al., 1996)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	500-bp eve stripe 3 lacZ (Keller et al., 2000) eve3+7-lacZ (Andrioli et al., 2002, Clyde et al., 2003) eve stripe 3/7 lacZ (Struffi et al., 2004) eve stripe 3+7 (Jennings et al., 2004) P{eve-lacZ.C} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Garcia and Stathopoulos, 2011, PLoS ONE 6(12): e29172 Lateral gene expression in Drosophila early embryos is supported by grainyhead-mediated activation and tiers of dorsally-localized repression. [FBrf0217082] Jennings et al., 2004, Curr. Biol. 14(18): 1680--1684 Locus-specific requirements for Spt5 in transcriptional activation and repression in Drosophila. [FBrf0180063] Struffi et al., 2004, Development 131(10): 2419--2429 Quantitative contributions of CtBP-dependent and -independent repression activities of Knirps. [FBrf0174586] Clyde et al., 2003, Nature 426(6968): 849--853 A self-organizing system of repressor gradients establishes segmental complexity in Drosophila. [FBrf0167932] Andrioli et al., 2002, Development 129(21): 4931--4940 Anterior repression of a Drosophila stripe enhancer requires three position-specific mechanisms. [FBrf0151930] Keller et al., 2000, Mol. Cell. Biol. 20(19): 7247--7258 dCtBP-dependent and -independent repression activities of the Drosophila Knirps protein. [FBrf0129892] Small et al., 1996, Dev. Biol. 175(2): 314--324 Regulation of two pair-rule stripes by a single enhancer in the Drosophila embryo. [FBrf0087716] Yan et al., 1996, Cell 84(3): 421--430 Identification of a Stat gene that functions in Drosophila development. [FBrf0086692]