Recombinant construct P{UAS-Egfr.DN}

General Information
Symbol	P{UAS-Egfr.DN}	FlyBase ID	FBtp0007539
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Freeman, 1996)
Uses	CV term Qualifiers & info Reference binary system (targeted) Egfr (Freeman, 1996) characterization Egfr (Freeman, 1996)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Freeman, 1996)
Component Alleles
Allele	Egfr^DN.Scer\UAS
Reference(s)	(Freeman, 1996, O'Keefe et al., 1997, Reich et al., 1999, Alvarez et al., 2003, Carmena et al., 2002, Bergmann et al., 2002, Hidalgo et al., 2001, Lin et al., 2003, Duchek et al., 2001, Kumar and Moses, 2001, Gilboa and Lehmann, 2006, Nakagoshi et al., 2002, Fulga and Rorth, 2002, Held, 2002, Sen et al., 2004, Gallio et al., 2004, Alvarado et al., 2004, Brumby and Richardson, 2003, Urban et al., 2004, Elstob et al., 2001, Zhao et al., 2000, Kumar and Moses, 2001, Duchek and Rorth, 2001, Bruckner et al., 2004, Flores et al., 2000, Jhaveri et al., 2000, Martin-Bermudo, 2000, Lanoue et al., 2000, Szuts and Bienz, 2000, Lanoue and Jacobs, 1999, Payre et al., 1999, Kopp et al., 1999, Spencer et al., 1998, zur Lage and Jarman, 1999, Ghiglione et al., 1999, Chen and Chien, 1999, Casci et al., 1999, Huang et al., 1998, Yarnitzky et al., 1998, Ting et al., 2005, Nagaraj et al., 1999, Szuts et al., 1997, Kubota et al., 2000, Yarnitzky et al., 1997, Xu et al., 2000, Bajpai et al., 2004, Inbal et al., 2004, Szuts et al., 1998, Brown and Freeman, 2003, Shirai et al., 2003, Sepp and Auld, 2003, Scholz et al., 1997, Pallavi and Shashidhara, 2003, Ghiglione et al., 2003, Scholz et al., 1997, Freeman, 1997, Parker, 2006, McDonald et al., 2006, Pallavi et al., 2006, Li and Carthew, 2005, Cela and Llimargas, 2006, Charroux et al., 2006, Li et al., 2009, Srahna et al., 2006, Bruckner et al., 2004, Sprecher et al., 2007, Zettl et al., 2011, Inaki et al., 2012, Corl et al., 2009, Yan et al., 2009, Lyulcheva et al., 2008, Wang et al., 2008, Martin-Pena et al., 2006, Donlea et al., 2009, Mallik and Lakhotia, 2009, Robertson et al., 2000, Wang et al., 2010, Wang et al., 2010, Egoz-Matia et al., 2011, Wojcinski et al., 2011, Deng et al., 2010, Chang et al., 2008, Wang et al., 2006)
Molecular data	A type I Egfr cDNA, with a termination codon 13 amino acids C-terminal to the transmembrane domain, is expressed under the control of Scer\UAS regulatory sequences. (O'Keefe et al., 1997) A dominant negative form of Egfr (that lacks the cytoplasmic kinase domain) is expressed under the control of Scer\UAS regulatory sequences. (Szuts et al., 1997) Construct: Expression of Egfr fragment, truncated 13 amino acids C-terminal of the transmembrane domain, is influenced by Scer\UAS Scer\GAL4 binding sites. (Freeman, 1996)
Phenotypic class	cell polarity defective, with Scer\GAL4^hs.PB (Brown and Freeman, 2003) cell size defective, with Scer\GAL4⁷⁹⁶ (Martin-Pena et al., 2006) chemical sensitive, with Scer\GAL4^Ilp2.PR (Corl et al., 2009) decreased cell number \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) increased cell death, with Scer\GAL4^hs.PB (Kurada and White, 1998) increased cell death, with Scer\GAL4^prd.RG1 (Urban et al., 2004) increased cell death \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) increased cell death \| second instar larval stage, with Scer\GAL4^C587 (Gilboa and Lehmann, 2006) increased cell number, with Scer\GAL4^C587 (Gilboa and Lehmann, 2006) increased cell size \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) lethal, with Scer\GAL4^ple.PF (Corl et al., 2009) lethal, with Scer\GAL4^sca-537.4 (Held, 2002) lethal \| embryonic stage, with Scer\GAL4^sca-537.4 (Chen and Chien, 1999) neuroanatomy defective (Ting et al., 2005) neuroanatomy defective \| embryonic stage, with Scer\GAL4^repo (Sepp and Auld, 2003) partially lethal - majority die, with Scer\GAL4^GMR.PF (Corl et al., 2009) size defective, with Scer\GAL4^Bx-MS1096 (Lyulcheva et al., 2008) visible, with Scer\GAL4^Act5C.PI (Kumar and Moses, 2001) visible, with Scer\GAL4^Bx-MS1096 (Egoz-Matia et al., 2011) visible, with Scer\GAL4^GMR.PF (Lin et al., 2003, Zettl et al., 2011) visible, with Scer\GAL4^T155 (Kopp et al., 1999)
Phenotype manifest in	abdominal lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^en-e16E (Elstob et al., 2001) antenna, with Scer\GAL4^Act5C.PI (Kumar and Moses, 2001) antenna & neuron \| conditional ts, with Scer\GAL4^hs.PB (Sen et al., 2004) Bolwig organ, with Scer\GAL4^so-7.GAL4 (Sprecher et al., 2007) border follicle cell, with Scer\GAL4^slbo.2.6 (Duchek and Rorth, 2001, Duchek et al., 2001) border follicle cell & filopodium, with Scer\GAL4^slbo.2.6 (Fulga and Rorth, 2002) chordotonal organ precursor cell & ventral thoracic disc, with Scer\GAL4^sca-109-68 (zur Lage and Jarman, 1999) chorion, with Scer\GAL4^T155 (Ghiglione et al., 1999) commissure, with Scer\GAL4^sim.PS (Scholz et al., 1997, Scholz et al., 1997) cone cell, with Scer\GAL4^hs.PB (Freeman, 1996) denticle belt, with Scer\GAL4^69B (Payre et al., 1999) denticle belt, with Scer\GAL4^arm.PS (Szuts et al., 1997) denticle belt, with Scer\GAL4^e22c (Payre et al., 1999) denticle belt, with Scer\GAL4^prd.RG1 (Urban et al., 2004) denticle row 1, with Scer\GAL4^en-e16E (O'Keefe et al., 1997) dorsal acute muscle, with Scer\GAL4^twi.PB (Carmena et al., 2002) dorsal oblique muscle, with Scer\GAL4^twi.PB (Carmena et al., 2002) embryo, with Scer\GAL4^hs.PB (Kurada and White, 1998) embryonic/larval ganglionic branch, with Scer\GAL4^bs-23.26 (Gallio et al., 2004) embryonic/larval oenocyte, with Scer\GAL4^en-e16E (Elstob et al., 2001) embryonic/larval oenocyte, with Scer\GAL4^salm-459.2 (Elstob et al., 2001) embryonic epidermis, with Scer\GAL4^69B (Payre et al., 1999) embryonic epidermis, with Scer\GAL4^e22c (Payre et al., 1999) embryonic segment, with Scer\GAL4^en-e16E (Parker, 2006) embryonic trachea & cortical actin cytoskeleton, with Scer\GAL4^btl.PS (Cela and Llimargas, 2006) endoderm, with Scer\GAL4^48Y (Szuts et al., 1998) eye, with Scer\GAL4^Act5C.PI (Kumar and Moses, 2001) eye, with Scer\GAL4^GMR.PF (Lin et al., 2003, Wang et al., 2008, Mallik and Lakhotia, 2009, Zettl et al., 2011) eye-antennal disc, with Scer\GAL4^GMR.PF (Freeman, 1996) eye photoreceptor cell, with Scer\GAL4^GMR.PF (Freeman, 1996) fascicle \| embryonic stage, with Scer\GAL4^repo (Sepp and Auld, 2003) gastric caecum, with Scer\GAL4^how-24B (Szuts et al., 1998) germline stem cell \| supernumerary, with Scer\GAL4^C587 (Gilboa and Lehmann, 2006) interface glial cell, with Scer\GAL4^htl.POS (Hidalgo et al., 2001) lamina monopolar neuron L1 (Ting et al., 2005) lamina monopolar neuron L2 (Ting et al., 2005) lamina monopolar neuron L3 (Ting et al., 2005) lamina monopolar neuron L4 (Ting et al., 2005) lamina monopolar neuron L5 (Ting et al., 2005) larval head, with Scer\GAL4^arm.PS (Szuts et al., 1997) lch5 ligament attachment cell, with Scer\GAL4^69B (Inbal et al., 2004) lch5 ligament attachment cell, with Scer\GAL4^sr-md710 (Inbal et al., 2004) medial longitudinal fascicle, with Scer\GAL4^htl.POS (Hidalgo et al., 2001) mesothoracic tergum, with Scer\GAL4^{Ubx-lac1-Gal4} (Pallavi and Shashidhara, 2003) mesothoracic ventral denticle belt, with Scer\GAL4^arm.PS (Szuts et al., 1997) metathoracic ventral denticle belt, with Scer\GAL4^arm.PS (Szuts et al., 1997) midgut constriction 1, with Scer\GAL4^how-24B (Szuts et al., 1998) midgut constriction 2, with Scer\GAL4^how-24B (Szuts et al., 1998) midgut constriction 3, with Scer\GAL4^how-24B (Szuts et al., 1998) midline glial cell, with Scer\GAL4^sli.PS (Bergmann et al., 2002) morphogenetic furrow, with Scer\GAL4^dpp.blk1 (Kumar and Moses, 2001) motor neuron \| embryonic stage, with Scer\GAL4^repo (Sepp and Auld, 2003) ommatidium, with Scer\GAL4^hs.PB (Brown and Freeman, 2003) optic cartridge, with Scer\GAL4^αTub84B.PP (Huang et al., 1998) photoreceptor cell, with Scer\GAL4^GMR.PF (Li and Carthew, 2005) photoreceptor cell R1, with Scer\GAL4^hs.2sev (Freeman, 1996) photoreceptor cell R3, with Scer\GAL4^hs.2sev (Freeman, 1996) photoreceptor cell R4, with Scer\GAL4^hs.2sev (Freeman, 1996) photoreceptor cell R6, with Scer\GAL4^hs.2sev (Freeman, 1996) photoreceptor cell R7, with Scer\GAL4^hs.2sev (Freeman, 1996) photoreceptor cell R7, with Scer\GAL4^hs.PB (Freeman, 1996) presumptive embryonic/larval peripheral nervous system, with Scer\GAL4^repo (Sepp and Auld, 2003) primary pigment cell, with Scer\GAL4^hs.PB (Freeman, 1996) retina, with Scer\GAL4^GMR.PF (Freeman, 1996, Robertson et al., 2000) secondary pigment cell, with Scer\GAL4^hs.PB (Freeman, 1996) sensillum basiconicum, with Scer\GAL4^lz-gal4 (Jhaveri et al., 2000) sensillum basiconicum, with Scer\GAL4^sca-P309 (Jhaveri et al., 2000) sensillum coeloconicum \| ectopic, with Scer\GAL4^lz-gal4 (Jhaveri et al., 2000) sensillum coeloconicum \| ectopic, with Scer\GAL4^sca-P309 (Jhaveri et al., 2000) sensillum trichodeum, with Scer\GAL4^lz-gal4 (Jhaveri et al., 2000) sensillum trichodeum, with Scer\GAL4^sca-P309 (Jhaveri et al., 2000) sensory neuron & axon & embryo, with Scer\GAL4^repo (Sepp and Auld, 2003) sternite, with Scer\GAL4^T155 (Kopp et al., 1999) tarsal segment, with Scer\GAL4^Dll-md23 (Held, 2002) tergite, with Scer\GAL4^T155 (Kopp et al., 1999) tertiary pigment cell, with Scer\GAL4^hs.PB (Freeman, 1996) tibia, with Scer\GAL4^Dll-md23 (Held, 2002) tracheal branch primordium, with Scer\GAL4^btl.PS (Cela and Llimargas, 2006) ventral thoracic disc primordium, with Scer\GAL4^Dll-md23 (Kubota et al., 2000) wing, with Scer\GAL4^Bx-MS1096 (Lyulcheva et al., 2008) wing, with Scer\GAL4^{Ubx-lac1-Gal4} (Pallavi and Shashidhara, 2003) wing cell (Yan et al., 2009) wing disc \| dorsal compartment, with Scer\GAL4^A9 (Nagaraj et al., 1999) wing vein (Yan et al., 2009) wing vein, with Scer\GAL4^GMR.PF (Charroux et al., 2006) wing vein L2, with Scer\GAL4^GMR.PF (Robertson et al., 2000) wing vein L3, with Scer\GAL4^Bx-MS1096 (Egoz-Matia et al., 2011) wing vein L3, with Scer\GAL4^GMR.PF (Robertson et al., 2000) wing vein L4, with Scer\GAL4^Bx-MS1096 (Egoz-Matia et al., 2011) wing vein L4, with Scer\GAL4^GMR.PF (Robertson et al., 2000) wing vein L5, with Scer\GAL4^Bx-MS1096 (Egoz-Matia et al., 2011) wing vein L5, with Scer\GAL4^GMR.PF (Robertson et al., 2000)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	DN-DER (Fulga and Rorth, 2002) P[UAS-DNDER] (Lanoue et al., 2000) P[UAS-dnDER] (Lanoue and Jacobs, 1999) P[UAS-DN-EGFR] (Huang et al., 1998) P[UAS-EGFR^DN] (Lin et al., 2003) P{UAS-DNegfr} (Alvarado et al., 2004) P{UAS-Egfr.DN} (FlyBase, 1996-) P{w⁺,DER^DN} (Spencer et al., 1998) P^{UAS-EGFR<up>DN}</up> (Wang et al., 2010, Chang et al., 2008) P^UAS-EGFR-DN (Wang et al., 2006) UAS.DN-DER (Szuts and Bienz, 2000) UAS-DER^DN (Shirai et al., 2003, Nakagoshi et al., 2002, Xu et al., 2000) UAS-DERDN (Payre et al., 1999) UAS-DERdn (Bruckner et al., 2004, Bruckner et al., 2004) UAS-DN-DER (Bajpai et al., 2004, Inbal et al., 2004, Sen et al., 2004, Pallavi and Shashidhara, 2003, Duchek et al., 2001, Duchek and Rorth, 2001, Jhaveri et al., 2000, Kopp et al., 1999, Chen and Chien, 1999, Pallavi et al., 2006, Egoz-Matia et al., 2011) UAS-DNDER (Carmena et al., 2002, Yarnitzky et al., 1998) UAS-DN DER (Reich et al., 1999) UAS-DN-EGFR (Urban et al., 2004, McDonald et al., 2006, Inaki et al., 2012) UAS-DN-Egfr (Brown and Freeman, 2003, Lyulcheva et al., 2008) UAS-DNEgfr (Alvarez et al., 2003, Zettl et al., 2011) UASDN-Egfr (Casci et al., 1999) UAS-DN-egfr (Deng et al., 2010) UAS-EGFR.DN (Kubota et al., 2000, Li and Carthew, 2005, Li et al., 2009) UAS-Egfr.DN (Robertson et al., 2000) UAS-EGFR^DN (Ting et al., 2005, Bergmann et al., 2002, Elstob et al., 2001, Flores et al., 2000, zur Lage and Jarman, 1999, Nagaraj et al., 1999, Charroux et al., 2006, Yan et al., 2009, Wang et al., 2008, Wang et al., 2010, Wojcinski et al., 2011) UAS-Egfr^DN (Brumby and Richardson, 2003, Sepp and Auld, 2003, Held, 2002, Hidalgo et al., 2001, Kumar and Moses, 2001, Kumar and Moses, 2001, Cela and Llimargas, 2006, Martin-Pena et al., 2006, Donlea et al., 2009) UAS-EGFR^dn (Sprecher et al., 2007) UAS-egfr^DN (Corl et al., 2009) UAS-EgfrDN (Gallio et al., 2004, Martin-Bermudo, 2000, Gilboa and Lehmann, 2006) UAS-EGFRDN (Ghiglione et al., 1999) UAST-DERDN (Ghiglione et al., 2003) U-DN-Egfr (Zettl et al., 2011)
Secondary FlyBase IDs

References ( 83 )

Generate a list of	All references relating to this transgenic transposon ( 83 )

List References by type	Research paper ( 78 ) Supplementary material ( 3 ) Review ( 1 ) Erratum ( 1 )
Recent research papers ( 4 )
	Inaki et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(6): 2027--2032 Effective guidance of collective migration based on differences in cell states. [FBrf0217439] Egoz-Matia et al., 2011, Dev. Biol. 351(1): 99--109 Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression. [FBrf0213026] Wojcinski et al., 2011, Dev. Biol. 358(1): 168--180 DSulfatase-1 fine-tunes Hedgehog patterning activity through a novel regulatory feedback loop. [FBrf0215253] Zettl et al., 2011, Cell 145(1): 79--91 Rhomboid Family Pseudoproteases Use the ER Quality Control Machinery to Regulate Intercellular Signaling. [FBrf0213387] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...