Recombinant construct P{NEE-2xh50-lacZ}

General Information
Symbol	P{NEE-2xh50-lacZ}	FlyBase ID	FBtp0007920
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Barolo and Levine, 1997)
Uses	CV term Qualifiers & info Reference reporter construct h (Barolo and Levine, 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Barolo and Levine, 1997)
Component Alleles
Allele	Ecol\lacZ^{rho.NEE.2xh.50}
Reference(s)	(Nibu et al., 1998, Barolo and Levine, 1997)
Molecular data	Construct: Two high affinity h binding sites are added, one 50bp upstream of d1 and the second 50bp downstream of d4. (Barolo and Levine, 1997)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^{rho.NEE.2xh.50}
Reference(s)	(Nibu et al., 1998, Barolo and Levine, 1997)
Molecular data	Construct: 700bp rho enhancer fragment with two high affinity h binding sites are added, one 50bp upstream of d1 and the second 50bp downstream of d4, drives w expression. (Barolo and Levine, 1997)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{NEE-2xh50-lacZ} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 2 )
Research paper	Nibu et al., 1998, EMBO J. 17(23): 7009--7020 dCtBP mediates transcriptional repression by knirps, Kruppel and snail in the Drosophila embryo. [FBrf0105894] Barolo and Levine, 1997, EMBO J. 16(10): 2883--2891 hairy mediates dominant repression in the Drosophila embryo. [FBrf0093352]