Recombinant construct P{ph-d^+tSa}

General Information
Symbol	P{ph-d^+tSa}	FlyBase ID	FBtp0007943
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Saget and Randsholt, 1994)
Uses	CV term Qualifiers & info Reference characterization ph-d (Saget and Randsholt, 1994) rescue construct ph-d (Saget and Randsholt, 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference complete rescue (Saget and Randsholt, 1994) selectable marker (Saget and Randsholt, 1994)
Component Alleles
Allele	ph-d^+tSa
Reference(s)	(Saget and Randsholt, 1994, Randsholt et al., 2000, Wang et al., 2006, Feng et al., 2011)
Molecular data	Construct: Fragment containing the ph-d locus (nucleotides 14485 to 28627). (Saget and Randsholt, 1994)
Phenotypic class	wild-type (Saget and Randsholt, 1994)
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Saget and Randsholt, 1994)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[phd⁺] (Randsholt et al., 2000) P{ph-d⁺} (Wang et al., 2006, Feng et al., 2011) P{ph-d^+tSa} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 4 )
Research paper	Feng et al., 2011, EMBO Rep. 12(2): 157--163 Loss of the Polycomb group gene polyhomeotic induces non-autonomous cell overproliferation. [FBrf0214189] Wang et al., 2006, Development 133(7): 1231--1240 Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain. [FBrf0190330] Randsholt et al., 2000, Mech. Dev. 95(1-2): 89--99 polyhomeotic controls engrailed expression and the hedgehog signaling pathway in imaginal discs. [FBrf0130036] Saget and Randsholt, 1994, Gene 149(2): 227--235 Transposon-induced rearrangements in the duplicated locus ph of Drosophila melanogaster can create new chimeric genes functionally identical to the wild type. [FBrf0076487]

Recombinant construct P{ph-d+tSa}

Recombinant construct P{ph-d^+tSa}