A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Recombinant construct P{UAS-Rho1.N19}

General Information
Symbol P{UAS-Rho1.N19} FlyBase ID FBtp0008154
Feature type transgenic_transposon
Size Expression data
Associated insertions 3 available
Molecular map
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Description
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FB2013_03
FB2013_02
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hide Description & Uses
Species synthetic construct
Location-dependent
role
Description
CV term
Qualifiers & info
Reference
transposon
P-element
(Strutt et al., 1997)
Uses
CV term
Qualifiers & info
Reference
binary system (targeted)
Rho1
(Strutt et al., 1997)
characterization
Rho1
(Strutt et al., 1997)
Cloning Sites
Location
Restriction sites
Reference
hide Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
hide Segments & Size
Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
hide Features
CV term
Qualifiers & info
Reference
binary
component
(Strutt et al., 1997)
hide Component Alleles
Allele Rho1N19.Scer\UAS
Reference(s) (Denholm et al., 2005, Grzeschik and Knust, 2005, Barrett et al., 1997, Yan et al., 2009, Bashaw et al., 2001, Prokopenko et al., 1999, Sepp and Auld, 2003, Lee and Kolodziej, 2002, Bloor and Kiehart, 2001, Harden et al., 2002, Bloor and Kiehart, 2002, Simoes et al., 2006, Schock and Perrimon, 2002, Jacinto et al., 2002, Fritz and VanBerkum, 2002, Kunwar et al., 2003, Fan et al., 2003, Hacker and Perrimon, 1998, Martin-Bermudo et al., 1999, Harden et al., 1999, Lu and Settleman, 1999, Sotillos and Campuzano, 2000, Schumacher et al., 2004, Strutt et al., 1997, Huelsmann et al., 2006, Emery et al., 2005, Stramer et al., 2005, Hozumi et al., 2006, Srahna et al., 2006, Lovegrove et al., 2006, Taniguchi et al., 2007, Neisch et al., 2010, von Hilchen et al., 2010, Massarwa et al., 2009, Xu et al., 2008, Paladi and Tepass, 2004, Widmann and Dahmann, 2009, Bastock and Strutt, 2007, Yu et al., 2010, Sato et al., 2010, Zeng et al., 2010, Song and Giniger, 2011, He et al., 2010, Matsubara et al., 2011, Renault et al., 2010, Elsaesser et al., 2010)
Molecular data
Construct: A dominant negative form of Rho1 is expressed under the control of Scer\UAS regulatory sequences.
(Strutt et al., 1997)
Phenotypic class
cell migration defective | embryonic stage 10, with Scer\GAL4nos.UTR.T:Hsim\VP16
(Renault et al., 2010)
cytokinesis defective, with Scer\GAL4neur-P72
(Emery et al., 2005)
cytokinesis defective, with Scer\GAL4twi.PB
(Schumacher et al., 2004)
increased cell death, with Scer\GAL4dpp.blk1
(Neisch et al., 2010)
lethal, with Scer\GAL4elav.PLu
(Fritz and VanBerkum, 2002)
lethal | embryonic stage, with Scer\GAL4en-e16E
(Bloor and Kiehart, 2002)
lethal | embryonic stage, with Scer\GAL4prd.RG1
(Bloor and Kiehart, 2002)
lethal | embryonic stage, with Scer\GAL4repo
(Sepp and Auld, 2003)
mitotic cell cycle defective, with Scer\GAL4prd.RG1
(Prokopenko et al., 1999)
planar polarity defective, with Scer\GAL4dpp.blk1
(Strutt et al., 1997)
Phenotype manifest in
amnioproctodeal invagination, with Scer\GAL4nos.PG
(Barrett et al., 1997)
anterior midgut primordium, with Scer\GAL4twi.PG
(Hacker and Perrimon, 1998)
border follicle cell, with Scer\GAL4slbo.2.6
(Bastock and Strutt, 2007)
cortical actin cytoskeleton & embryonic posterior spiracle, with Scer\GAL4ems.HRE
(Simoes et al., 2006)
dorsal closure embryo, with Scer\GAL4en-e16E
(Bloor and Kiehart, 2002)
dorsal closure embryo, with Scer\GAL4LE
(Lu and Settleman, 1999)
dorsal closure embryo, with Scer\GAL4pnr-MD237
(Schock and Perrimon, 2002)
dorsal closure embryo, with Scer\GAL4prd.RG1
(Bloor and Kiehart, 2002)
dorsal trunk primordium, with Scer\GAL4btl.PS
(Lee and Kolodziej, 2002)
embryonic/first instar larval cuticle, with Scer\GAL4en-e16E
(Bloor and Kiehart, 2002)
embryonic/first instar larval cuticle, with Scer\GAL4mat.αTub67C.T:Hsim\VP16
(Hacker and Perrimon, 1998)
embryonic/first instar larval cuticle, with Scer\GAL4prd.RG1
(Bloor and Kiehart, 2002)
embryonic/first instar larval cuticle, with Scer\GAL4twi.PG
(Hacker and Perrimon, 1998)
embryonic/first instar larval cuticle | dorsal, with Scer\GAL4hs.PB
(Harden et al., 1999)
embryonic/larval hindgut, with Scer\GAL4NP2432
(Taniguchi et al., 2011)
embryonic/larval midgut primordium, with Scer\GAL4NP2432
(Hozumi et al., 2006)
embryonic epidermis, with Scer\GAL4en-e16E
(Bloor and Kiehart, 2002)
embryonic epidermis, with Scer\GAL4prd.RG1
(Bloor and Kiehart, 2002)
embryonic epidermis | dorsal, with Scer\GAL4LE
(Lu and Settleman, 1999)
embryonic hemocyte, with Scer\GAL4Pxn.PS, Scer\GAL4crq.PA
(Stramer et al., 2005)
embryonic hindgut, with Scer\GAL4NP2432
(Hozumi et al., 2006, Taniguchi et al., 2007)
embryonic leading edge cell, with Scer\GAL4en-e16E
(Jacinto et al., 2002, Bloor and Kiehart, 2002)
embryonic leading edge cell, with Scer\GAL4prd.RG1
(Bloor and Kiehart, 2002)
embryonic Malpighian tubule tip cell, with Scer\GAL4ct.CtB
(Denholm et al., 2005)
extended germ band embryo, with Scer\GAL4c381
(Schock and Perrimon, 2002)
extended germ band embryo, with Scer\GAL4Kr.PM
(Schock and Perrimon, 2002)
external sensory organ precursor cell, with Scer\GAL4neur-P72
(Emery et al., 2005)
eye photoreceptor cell | third instar larval stage, with Scer\GAL4GMR.PF
(Yu et al., 2010)
filopodium, with Scer\GAL4en-e16E
(Jacinto et al., 2002)
filzkorper, with Scer\GAL4ems.HRE
(Simoes et al., 2006)
germline cell | ectopic | embryonic stage | maternal effect, with Scer\GAL4nos.UTR.T:Hsim\VP16
(Kunwar et al., 2003)
germline cell | embryonic stage, with Scer\GAL4nos.UTR.T:Hsim\VP16
(Renault et al., 2010)
imaginal disc | third instar larval stage, with Scer\GAL4dpp.blk1
(Neisch et al., 2010)
lamina | third instar larval stage, with Scer\GAL4GMR.PF
(Yu et al., 2010)
mesectoderm, with Scer\GAL4en-e16E
(Bloor and Kiehart, 2002)
mesoderm, with Scer\GAL4twi.PG
(Hacker and Perrimon, 1998)
peripheral glial cell, with Scer\GAL4repo
(Sepp and Auld, 2003)
posterior spiracle primordium, with Scer\GAL4ems.HRE
(Simoes et al., 2006)
presumptive embryonic/larval muscle system, with Scer\GAL4how-24B
(Bloor and Kiehart, 2001)
presumptive embryonic salivary gland, with Scer\GAL4en-e16E
(Xu et al., 2008)
presumptive embryonic salivary gland, with Scer\GAL4fkh.PH
(Xu et al., 2008)
presumptive embryonic salivary gland, with Scer\GAL4twi.PB
(Xu et al., 2008)
primordium of embryonic/larval spiracle, with Scer\GAL4ems.HRE
(Lovegrove et al., 2006)
stomodeal invagination, with Scer\GAL4nos.PG
(Barrett et al., 1997)
ventral furrow, with Scer\GAL4nos.PG
(Barrett et al., 1997)
ventral longitudinal muscle, with Scer\GAL4how-24B
(Bloor and Kiehart, 2001)
wing | heat sensitive, with Scer\GAL4ptc-559.1, Scer\GAL80ts.αTub84B
(Yan et al., 2009)
wing | somatic clone, with Scer\GAL4Act.PU
(Yan et al., 2009)
wing hair, with Scer\GAL4dpp.blk1
(Strutt et al., 1997)
wing hair | heat sensitive, with Scer\GAL4ptc-559.1, Scer\GAL80ts.αTub84B
(Yan et al., 2009)
wing hair | supernumerary | heat sensitive, with Scer\GAL4ptc-559.1, Scer\GAL80ts.αTub84B
(Yan et al., 2009)
Other information
Allele w+mC
Reference(s) (Strutt et al., 1997)
Molecular data
Minigene of the white cDNA.
(Chung et al., 1993)
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
(Pirrotta, 1988)
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
 
Phenotypic class
courtship behavior defective | male
(Lloyd et al., 2002, Hing and Carlson, 1996)
eye color defective
(Soldatov et al., 1999, Hagstrom et al., 1996)
eye color defective, with Scer\GAL4Act5C.PI, wdsRNA.Sym.Scer\UAS
(Giordano et al., 2002)
visible | dominant
(True et al., 1996)
visible | recessive
(Roseman et al., 1993)
Phenotype manifest in
pigment cell
(Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995)
pigment cell, with Scer\GAL4Act5C.PI, wdsRNA.Sym.Scer\UAS
(Giordano et al., 2002)
Other information
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects expression of
hide Progenitors & Descendants
progenitor(s)
descendant(s)
hide Comments
hide Stocks Listed in FlyBase ( 2 )
Bloomington
7327
P{UAS-Rho1.N19}1.3, w*
7328
w*; P{UAS-Rho1.N19}2.1
hide External Crossreferences & Linkouts
Other Crossreferences
Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
p[w+,RhoAN19]
(Bloor and Kiehart, 2002)
P{UAS-Rho1.N19}
(FlyBase, 1996-)
P{UAS-Rho1N19}
(FlyBase, 1996-)
PUAS-Rho1<up>N19</up>
(Elsaesser et al., 2010)
pUAST-RhoAN19
(Lee and Kolodziej, 2002)
rho1N17
(Schock and Perrimon, 2002)
Rho1N19
(Lu and Settleman, 1999, Taniguchi et al., 2007, Renault et al., 2010)
RhoAN19
(Bastock and Strutt, 2007)
UAS::Rho1N19
(Schumacher et al., 2004)
UAS-DRho
(Sotillos and Campuzano, 2000)
UAS-DRhoAN19
(Harden et al., 1999)
UAS-Rho1
(Neisch et al., 2010)
UAS-Rho1.N19
(von Hilchen et al., 2010, Yu et al., 2010)
UAS-Rho1N19
(Kunwar et al., 2003, Prokopenko et al., 1999, Neisch et al., 2010, Massarwa et al., 2009, Xu et al., 2008, Paladi and Tepass, 2004, Widmann and Dahmann, 2009, Song and Giniger, 2011)
UAS-rho1N19
(Jacinto et al., 2002)
UAS Rho1N19
(Renault et al., 2010)
UASrho1N19
(Renault et al., 2010)
UAS-Rho1T19N
(Sato et al., 2010)
UAS-Rho1 N19
(Yan et al., 2009)
UAS-Rho.N19
(Denholm et al., 2005)
UAS-RhoN19
(Grzeschik and Knust, 2005, Martin-Bermudo et al., 1999)
UASrhoN19
(Schock and Perrimon, 2002)
UAS-rhoN19
(Huelsmann et al., 2006)
UAS-RhoAN19
(Bloor and Kiehart, 2001, Zeng et al., 2010)
UAS-RhoAT19N
(Matsubara et al., 2011)
UAS-RhoAN19
(Sepp and Auld, 2003)
UASRhoN19
(Bashaw et al., 2001)
UAS-RhoN19
(Emery et al., 2005, Simoes et al., 2006, Lovegrove et al., 2006, He et al., 2010)
UAS-Rho N19
(Hozumi et al., 2006)
URhoN19
(Fan et al., 2003)
Secondary FlyBase IDs
hide References ( 46 )
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hide Recent research papers ( 2 )
Matsubara et al., 2011, Genes Dev. 25(18): 1982--1996
The seven-pass transmembrane cadherin Flamingo controls dendritic self-avoidance via its binding to a LIM domain protein, Espinas, in Drosophila sensory neurons. [FBrf0215818]
Song and Giniger, 2011, Dev. Dyn. 240(2): 324--332
Noncanonical notch function in motor axon guidance is mediated by Rac GTPase and the GEF1 domain of trio. [FBrf0212819]
Show all research papers ...