Recombinant construct P{BG-luc}

General Information
Symbol	P{BG-luc}	FlyBase ID	FBtp0008404
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Stanewsky et al., 1997)
Uses	CV term Qualifiers & info Reference characterization per (Stanewsky et al., 1997) reporter construct vital \| per (Stanewsky et al., 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Stanewsky et al., 1997) selectable marker (Stanewsky et al., 1997)
Component Alleles
Allele	per^{BG.T:Ppyr\LUC}
Reference(s)	(Stanewsky et al., 1997, Glaser and Stanewsky, 2005, Wuelbeck et al., 2005, Giebultowicz et al., 2000, Kaneko et al., 2000, Stanewsky et al., 1998, Belvin et al., 1999, Helfrich-Forster et al., 2001, Krishnan et al., 2001, Yuan et al., 2005, Koh et al., 2006, Dolezelova et al., 2007, Zheng et al., 2007, Hung et al., 2009, Sehadova et al., 2009, Stanewsky et al., 2002)
Molecular data	Construct: Fusion of a 9.8kb BamHI per fragment extending from -4200 to +5627 to a 1.8kb BamHI-KpnI Ppyr\LUC cDNA. Expression is influenced by 1kb EcoRI fragment carring the SV40 polyadenylation site. Two thirds of the per protein is fused in frame to the Ppyr\LUC cDNA. (Stanewsky et al., 1997)
Phenotypic class	locomotor rhythm defective (Glaser and Stanewsky, 2005)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Stanewsky et al., 1997)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	(BG)-luc (Kaneko et al., 2000) BG-luc (Wuelbeck et al., 2005, Giebultowicz et al., 2000, Krishnan et al., 2001, Belvin et al., 1999, Stanewsky et al., 1998, Koh et al., 2006, Stanewsky et al., 2002, Dolezelova et al., 2007, Sehadova et al., 2009, Zheng et al., 2007) BG-LUC (Yuan et al., 2005) BGluc (Hung et al., 2009) BG-luc60 (Helfrich-Forster et al., 2001) P{BG-luc} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 16 )
Research paper	Hung et al., 2009, J. Biol. Rhythms 24(3): 183--192 HSP90, a Capacitor of Behavioral Variation. [FBrf0207993] Sehadova et al., 2009, Neuron 64(2): 251--266 Temperature entrainment of Drosophila's circadian clock involves the gene nocte and signaling from peripheral sensory tissues to the brain. [FBrf0209187] Dolezelova et al., 2007, Genetics 177(1): 329--345 Rhythm defects caused by newly engineered null mutations in Drosophila's cryptochrome gene. [FBrf0201773] Zheng et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(40): 15899--15904 FOXO and insulin signaling regulate sensitivity of the circadian clock to oxidative stress. [FBrf0216135] Koh et al., 2006, Science 312(5781): 1809--1812 JETLAG resets the Drosophila circadian clock by promoting light-induced degradation of TIMELESS. [FBrf0193019] Glaser and Stanewsky, 2005, Curr. Biol. 15(15): 1352--1363 Temperature synchronization of the Drosophila circadian clock. [FBrf0187310] Wuelbeck et al., 2005, Genetics 169(2): 751--766 The novel Drosophila tim(blind) mutation affects behavioral rhythms but not periodic eclosion. [FBrf0187663] Yuan et al., 2005, Neuron 47(1): 115--127 Serotonin modulates circadian entrainment in Drosophila. [FBrf0188183] Stanewsky et al., 2002, J. Biol. Rhythms 17(4): 293--306 Mapping of elements involved in regulating normal temporal period and timeless RNA expression patterns in Drosophila melanogaster. [FBrf0151362] Helfrich-Forster et al., 2001, Neuron 30(1): 249--261 The circadian clock of fruit flies is blind after elimination of all known photoreceptors. [FBrf0135910] Krishnan et al., 2001, Nature 411(6835): 313--317 A new role for cryptochrome in a Drosophila circadian oscillator. [FBrf0135900] Giebultowicz et al., 2000, Curr. Biol. 10(2): 107--110 Transplanted Drosophila excretory tubules maintain circadian clock cycling out of phase with the host. [FBrf0125137] Kaneko et al., 2000, J. Neurobiol. 43(3): 207--233 Disruption of synaptic transmission or clock-gene-product oscillations in circadian pacemaker cells of Drosophila cause abnormal behavioral rhythms. [FBrf0128517] Belvin et al., 1999, Neuron 22(4): 777--787 The Drosophila dCREB2 gene affects the circadian clock. [FBrf0108141] Stanewsky et al., 1998, Cell 95(5): 681--692 The cryb mutation identifies cryptochrome as a circadian photoreceptor in Drosophila. [FBrf0105937] Stanewsky et al., 1997, EMBO J. 16(16): 5006--5018 Multiple circadian-regulated elements contribute to cycling period gene expression in Drosophila. [FBrf0096339]