FB2021_01, released Feb 18, 2021

Recombinant Construct: P{GAL4-lacZ-Ubx(FRT.f+).dC}

Open Close
General Information
Symbol
P{GAL4-lacZ-Ubx(FRT.f+).dC}
FlyBase ID
FBtp0008700
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZFRT.Ubx
Scer\GAL4FRT.Ubx
Also Known As
abx/ubx>f+>Gal4-lacZ, abx>f+>Gal4-lacZ
Size
Expression Data
lacZ reporter,GAL4 reporter/driver
Associated insertion(s)
1 available
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(de Celis and Bray, 1997)
Construct components
Product class / Tool use(s)
Marker(s)
f+t13
w+mC
Component allele Scer\GAL4FRT.Ubx
Product class / Tool use(s)
Regulatory region(s)
Ubx
Encoded product / tool
GAL4
Also carries
FRT
Description

An FRT cassette that contains a f+t13 marker separates the Ubx promoter from a coding cassette composed of GAL4 and lacZ (the two coding regions are separated by an internal ribosome entry site).

(de Celis and Bray, 1997)
Component allele Ecol\lacZFRT.Ubx
Product class / Tool use(s)
Regulatory region(s)
Ubx
Encoded product / tool
lacZ
Also carries
FRT
Description

An FRT cassette that contains a f+t13 marker separates the Ubx promoter from a coding cassette composed of GAL4 and lacZ (the two coding regions are separated by an internal ribosome entry site).

(de Celis and Bray, 1997)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
removable
cassette
(de Celis and Bray, 1997)
reporter
gene
(de Celis and Bray, 1997)
reporter
gene
(de Celis and Bray, 1997)
selectable
marker
(de Celis and Bray, 1997)
selectable
marker
(de Celis and Bray, 1997)
Component Alleles
Allele
Ecol\lacZFRT.Ubx
Reference(s)
(Whitworth and Russell, 2003, Moreno et al., 2002, Azpiazu and Morata, 2000, Baonza et al., 2000, de Celis and Bray, 2000, Dominguez, 1999, de Celis and Bray, 1997)
Molecular data

An FRT cassette that contains a f+t13 marker separates the Ubx promoter from a coding cassette composed of GAL4 and lacZ (the two coding regions are separated by an internal ribosome entry site).

(de Celis and Bray, 1997)
Phenotype Data
Phenotypic ClassPhenotype Manifest InDetailed Description
Allele
Scer\GAL4FRT.Ubx
Reference(s)
(Roy et al., 2011, Callejo et al., 2006, Hsiung, 2005.9.23, Hsiung et al., 2005, Cook et al., 2004, Lawrence et al., 2004, Li and Baker, 2004, Sakata, 2004, Torroja et al., 2004, Baena-Lopez and Garcia-Bellido, 2003, Azpiazu and Morata, 2002, Casal et al., 2002, Lawrence et al., 2002, Moreno et al., 2002, Moreno et al., 2002, Tree et al., 2002, Azpiazu and Morata, 2000, Baonza and Garcia-Bellido, 2000, Baonza et al., 2000, Calleja et al., 2000, de Celis and Bray, 2000, Mullor and Guerrero, 2000, Wang et al., 2000, Apidianakis et al., 1999, Dominguez, 1999, Lawrence et al., 1999, Phillips et al., 1999, Rauskolb and Irvine, 1999, de Celis and Bray, 1997)
Molecular data

An FRT cassette that contains a f+t13 marker separates the Ubx promoter from a coding cassette composed of GAL4 and lacZ (the two coding regions are separated by an internal ribosome entry site).

(de Celis and Bray, 1997)
Phenotype Data
Phenotypic ClassPhenotype Manifest InDetailed Description
Expression Data
Reporter Expression
Additional Information
Statement
Reference

Designed for clonal analysis in wing disc and adult wing; FRT-containing variant blocks Scer\GAL4 transcription. Expression is of Scer\GAL4Ubx.PdC, carried in descendent transposon P{GAL4-lacZ-Ubx(-FRT).dC}; see Scer\GAL4Ubx.PdC elsewhere in this table.

(FlyBase Curators, 2017)

Used as marker for clonal analysis in wing; FRT-containing variant blocks Ecol\lacZ expression; see expression data for P{GAL4-lacZ-Ubx(-FRT).dC} at Ecol\lacZUbx.PdCRA.

(de Celis and Bray, 1997)

Used for clonal analysis in wing; FRT-containing variant blocks Scer\GALZ expression; see expression data for P{GAL4-lacZ-Ubx(-FRT).dC} at Scer\GAL4Ubx.PdCRA.

(de Celis and Bray, 1997)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: Designed for clonal analysis in wing disc and adult wing: Ser\GAL4 expressed in cells that have lost the FRT.f+ element; used for clonal analysis the gene of interest, when appropriate UAS construct present; clones are simultaneously marked by expression of Ecol\lacZ in the imaginal disc and by f- phenotype in the adult wing.

(de Celis and Bray, 1997)
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (27)
Reported As
Symbol Synonym
10.1E
(Rauskolb and Irvine, 1999)
<up>abx FRT f+ > Gal4</up>
(Li and Baker, 2004)
P[abx/Ubx<FRT f+FRT>Gal4-lacZ]
(Baonza et al., 2000)
P{GAL4-lacZ-Ubx(FRT.f+).dC}
(FlyBase, 1996-)
P{GAL4-lacZ.FRT.dC}
(FlyBase, 1996-)
P{abx/Ubx<FRTf+FRT>Gal4-lacZ}
(de Celis and Bray, 2000)
Ubx-FRT<f+>Gal4
(Tree et al., 2002)
Ubx/abxFRT f+ FRT Gal4 lacZ
(Azpiazu and Morata, 2002)
Ubx<f+>GAL4-lacZ
(Whitworth and Russell, 2003)
Ubx> stop f+ >GAL4
(Apidianakis et al., 1999)
Ubx>f+>Gal4-lacZ
(Phillips et al., 1999)
Ubx>stop f+ >GAL4 lacZ
(Sakata, 2004)
ab>f+>Gal4-lacZ
(Cook et al., 2004)
abx/Ubx FRT f+ FRT G4 UAS lacZ
(Baena-Lopez and Garcia-Bellido, 2003)
abx/Ubx>stop,f+>GAL4-lacZ
(FlyBase, 1996-)
abx/pbx>f+>Gal4-lacZ
(Lawrence et al., 1999)
abx/ubx>f+>Gal4-lacZ
(Lawrence et al., 2004, Casal et al., 2002)
abx/ubx>f+>Gal4-lacZ
(Dominguez, 1999)
abx/ubx>f+Gal4-lacZ
(Lawrence et al., 2002)
abx>f+-stop>Gal4-lacZ
(Calleja et al., 2000)
abx>f+>Gal4-lacZ
(Moreno et al., 2002, Azpiazu and Morata, 2000)
abxubs>FRT>Gal4
(Hsiung, 2005.9.23)
abxubx>FRT>GAL4
(Roy et al., 2011)
abxubx>FRT>Gal4
(Hsiung et al., 2005)
pwA>f>GIL
(FlyBase, 1996-)
ubx>f+>Gal4
(Torroja et al., 2004)
ubx>f+>Gal4, UAS-βgal
(Callejo et al., 2006)
Secondary FlyBase IDs
    References (32)