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General Information
Symbol
P{UAS-Egfr.λtop}
FlyBase ID
FBtp0008722
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
UAS-λtop, UAS-EGFRλtop, UAS-λ-TOP, UAS-EGFRACT, UAS-dEGFRλ, UAS-λtop4.2, UAS-λDER, UAS-EGFR.λtop, UAS-Egfr.lambdatop, UAS-λ top
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Marker(s)
Component allele btl::Egfrλ.UAS
Product class / Tool use(s)
Description
UASt regulatory sequences drive expression of a constitutively active form of Egfr that consists of a signal peptide, the dimerization domain of the bacteriophage λ cI repressor (included to induce constitutive dimerization of the encoded protein), a 0.15kb of btl sequence that includes the transmembrane domain and a 1.9kb fragment that contains the 3' end of Egfr (this fragment includes the intracellular domain).
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
chimeric
gene
chimeric
protein
constitutive
activity
molecular
tag
selectable
marker
Component Alleles
Reference(s)
(Chen et al., 2019, Chi et al., 2019, Kim and Choi, 2019, La Marca et al., 2019, Mundorf et al., 2019, Portela et al., 2019, Wittes and Schüpbach, 2019, Frattini et al., 2018, Fernandes et al., 2017, Recasens-Alvarez et al., 2017, Requena et al., 2017, Johnston et al., 2016, Li et al., 2016, Bieli et al., 2015, Jin et al., 2015, Legent et al., 2015, Matzat et al., 2015, Reitman et al., 2015, Tavares et al., 2015, Wang et al., 2015, Caviglia and Luschnig, 2013, Cheung et al., 2013, Fregoso Lomas et al., 2013, Khan et al., 2013, Lin et al., 2013, Paul et al., 2013, Read et al., 2013, Butchar et al., 2012, Natori et al., 2012, Ajuria et al., 2011, Galindo et al., 2011, Giorgianni and Mann, 2011, Grigorian et al., 2011, Richter et al., 2011, Sinenko et al., 2011, Sun et al., 2011, Wang and Pai, 2011, Wojcinski et al., 2011, Xu et al., 2011, Deng et al., 2010, Roignant and Treisman, 2010, Almudi et al., 2009, Donlea et al., 2009, Jiang and Edgar, 2009, Leatherbarrow and Halfon, 2009, Li et al., 2009, Nagaraj and Banerjee, 2009, Read et al., 2009, Witte et al., 2009, Yan et al., 2009, Miura et al., 2008, Yakoby et al., 2008, Zartman et al., 2008, Astigarraga et al., 2007, Nagaraj and Banerjee, 2007, Cela and Llimargas, 2006, Charroux et al., 2006, Chen and Schupbach, 2006, Miura et al., 2006, Pai et al., 2006, Pallavi et al., 2006, Poulton and Deng, 2006, Wahlstrom et al., 2006, Cabernard and Affolter, 2005, Campbell, 2005, Hasson et al., 2005, Kristiansen et al., 2005, Li and Carthew, 2005, Lievens et al., 2005, Schober et al., 2005, Wilson et al., 2005, Zhu et al., 2005, Bajpai et al., 2004, Cordero et al., 2004, Gallio et al., 2004, Moreno and Basler, 2004, Gaengel and Mlodzik, 2003, Ghiglione et al., 2003, Lunde et al., 2003, Sepp and Auld, 2003, Xi et al., 2003, Brodu et al., 2002, Campbell, 2002, Dumstrei et al., 2002, Galindo et al., 2002, Galindo et al., 2002, Held, 2002, Peri et al., 2002, Prober and Edgar, 2002, Sudarsan et al., 2002, Tsuda et al., 2002, Zecca and Struhl, 2002, Duchek and Rorth, 2001, Johnson Hamlet and Perkins, 2001, Kumar and Moses, 2001, Kumar and Moses, 2001, Schnorr et al., 2001, Dobens et al., 2000, Flores et al., 2000, Luschnig et al., 2000, Martin-Bermudo, 2000, Sen et al., 2000, Wang et al., 2000, Xu et al., 2000, Zhao et al., 2000, Daniel et al., 1999, Ghiglione et al., 1999, Guichard et al., 1999, Kopp et al., 1999, Larkin et al., 1999, Reich et al., 1999, Wessells et al., 1999, Buff et al., 1998, Huang et al., 1998, Michelson et al., 1998, Miller and Cagan, 1998, Schupbach and Wieschaus, 1998, Spencer et al., 1998, Tinker et al., 1998, Queenan et al., 1997)
Molecular data
UASt regulatory sequences drive expression of a constitutively active form of Egfr that consists of a signal peptide, the dimerization domain of the bacteriophage λ cI repressor (included to induce constitutive dimerization of the encoded protein), a 0.15kb of btl sequence that includes the transmembrane domain and a 1.9kb fragment that contains the 3' end of Egfr (this fragment includes the intracellular domain).
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
construct_comment: Progenitors are those of the plasmid, pP{UAS-Egfr.λtop}.See report for T:λ\cIDD.
report comment: Produces active form of Egfr with btl transmembrane domain and λ\cIDD dimerization domain; not dependent upon ligand-induced dimerization and thus results in constitutive activity. Regulated by UAS-binding species present.
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (64)
Reported As
Symbol Synonym
EGFRλtop4.2
P[UAS-λ-EGFR]
P[w+, UAS-λEGFR]
P[w+,UAS-λ-DER]
P{UAS-Egfr.λtop}
P{UAS-λtop}
UAS-Activated EGFR
UAS-EGFR activated
UAS-EGFRλtop4.2
UAS-EGFRλtop4.2
UAS-Efgrλ4.2
UAS-Egf-r.λtop
UAS-Egfr*top4.2
UAS-Egfr.lambdatop
UAS-Egfr.λtop4.4
UAS-Egfrltop4
UAS-Egfr<up>top 4.2</up>
UAS-Egfr_Act
UAS-egfrλ-top
UAS-λ-egfr
UAS-λ-top 4.2
UAS-λ-topDER
UASEgfrλ4.2
activated-EGFR
Secondary FlyBase IDs
    References (122)