FlyBase Recombinant Construct Report: P{UAS-Egfr.λtop}

General Information

Symbol

P{UAS-Egfr.λtop}

FlyBase ID

FBtp0008722

Feature type

transgenic_transposable_element

Component Allele(s)

btl::Egfr^λ.UAS

Also Known As

UAS-λtop, UAS-EGFR^λtop, UAS-λ-TOP, UAS-EGFR^ACT, UAS-dEGFR^λ, UAS-dEGFRλ, UAS-λtop^4.2, UAS-λDER, UAS-EGFR.λtop, dEGFR^λ

Size

Expression Data

Associated insertion(s)

3 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele btl::Egfr^λ.UAS

Transgenic product class

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

btl::Egfr

Description

UASt regulatory sequences drive expression of a constitutively active form of Egfr that consists of a signal peptide, the dimerization domain of the bacteriophage λ cI repressor (included to induce constitutive dimerization of the encoded protein), a 0.15kb of btl sequence that includes the transmembrane domain and a 1.9kb fragment that contains the 3' end of Egfr (this fragment includes the intracellular domain).

(Queenan et al., 1997)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

btl::Egfr^λ.UAS

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{hs-λbtl}

(Queenan et al., 1997)

pP{UAST}

(Queenan et al., 1997)

descendant(s)

Comments

construct_comment: Progenitors are those of the plasmid, pP{UAS-Egfr.λtop}.See report for T:λ\cI^DD.

(Queenan et al., 1997)

report comment: Produces active form of Egfr with btl transmembrane domain and λ\cI^DD dimerization domain; not dependent upon ligand-induced dimerization and thus results in constitutive activity. Regulated by UAS-binding species present.

(Queenan et al., 1997)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

59843

w^*; P{UAS-Egfr.λtop}3/TM6C, Sb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (79)

Reported As

Symbol Synonym

EGFR-CA

(Fairchild et al., 2016)

EGFR^Act

(Estrada et al., 2006)

EGFR^λtop4.2

(Bieli et al., 2015)

EGFR^λtop

(Khan et al., 2013)

Egfr^Act

(Sinenko et al., 2011)

EgfrλTop

(Vialat et al., 2024)

P[UAS-λ-EGFR]

(Huang et al., 1998)

P[w⁺, UAS-λEGFR]

(Wilson et al., 2005)

P[w⁺,UAS-λ-DER]

(Spencer et al., 1998)

P{UAS-Egfr.λtop}

(FlyBase, 1996-)

P{UAS-λtop}

(FlyBase, 1996-)

UAS λtop

(Witte et al., 2009)

UAS-Activated EGFR

(Dumstrei et al., 2002)

UAS-EGFR activated

(Grigorian et al., 2011)

UAS-EGFR-λ

(Reitman et al., 2015)

UAS-EGFR.λtop

(Xu et al., 2011, Li et al., 2009, Li and Carthew, 2005)

UAS-EGFR^ACT

(Charroux et al., 2006, Brodu et al., 2002)

UAS-EGFR^CA

(Kim and Choi, 2019, Wojcinski et al., 2011)

UAS-EGFR^act

(Legent et al., 2015, Nagaraj and Banerjee, 2009, Nagaraj and Banerjee, 2007)

UAS-EGFR^top

(Jin et al., 2020, Pascual et al., 2017)

UAS-EGFR^λ

(Yan et al., 2009, Zecca and Struhl, 2002)

UAS-EGFR^λTOP4.2

(Recasens-Alvarez et al., 2017)

UAS-EGFR^λtop4.2

(Paul et al., 2013)

UAS-EGFR^λtop

(Legent et al., 2015, Khan et al., 2013, Lin et al., 2013, Roignant and Treisman, 2010, Miura et al., 2008, Prober and Edgar, 2002, Johnson Hamlet and Perkins, 2001)

UAS-EGFRlTop

(Vega-Cuesta et al., 2020)

UAS-EGFRλ

(Losada-Pérez et al., 2022, Portela et al., 2019)

UAS-EGFRλtop

(Johnston et al., 2016, Miura et al., 2006)

UAS-EGFRλtop4.2

(Requena et al., 2017)

UAS-Efgr^λ4.2

(Galindo et al., 2011)

UAS-Egf-r.λtop

(Pallavi et al., 2006)

UAS-Egfr*

(Donlea et al., 2009)

UAS-Egfr*^top4.2

(Held, 2002)

UAS-Egfr.lambdatop

(Campbell, 2005, Bajpai et al., 2004)

UAS-Egfr.λtop4.4

(Li et al., 2016)

UAS-Egfr^ACT

(Dong et al., 2021, Butchar et al., 2012)

UAS-Egfr^Act

(Sinenko et al., 2011)

UAS-Egfr^CA

(Li et al., 2020)

UAS-Egfr^ltop4

(Campbell, 2002)

UAS-Egfr<up>top 4.2</up>

(Kumar and Moses, 2001, Kumar and Moses, 2001)

UAS-Egfr^λtop

(Resnik-Docampo et al., 2021, Tavares et al., 2015, Natori et al., 2012)

UAS-Egfr_Act

(Sinenko et al., 2011)

UAS-Egfrλ

(La Marca et al., 2019)

UAS-dEGFR^λ

(Bertrand et al., 2023, Courant et al., 2022, Jarabo et al., 2022, Jarabo et al., 2021, Portela et al., 2020, Chen et al., 2019, Chi et al., 2019, Frattini et al., 2018, Read et al., 2009)

UAS-dEGFRλ

(Conte et al., 2021, Maravat et al., 2021, Ariss et al., 2020, Portela et al., 2019)

UAS-egfr^ACT

(Mundorf et al., 2019)

UAS-egfr^λ-top

(Tsuda et al., 2002)

UAS-lambdaEgfr

(Sepp and Auld, 2003, Martin-Bermudo, 2000)

UAS-lambdaTop

(Jiang et al., 2011)

UAS-lambdatop

(Levine et al., 2007, Estrada et al., 2006)

UAS-λ top

(Grigorian et al., 2011, Gallio et al., 2004)

UAS-λ-Egfr

(Li et al., 2015)

UAS-λ-TOP

(Hasson et al., 2005)

UAS-λ-egfr

(Deng et al., 2010)

UAS-λ-top

(Wang et al., 2015, Cheung et al., 2013, Wang and Pai, 2011, Bernardi et al., 2007, Chen and Schupbach, 2006, Gaengel and Mlodzik, 2003, Duchek and Rorth, 2001, Guichard et al., 1999)

UAS-λ-top 4.2

(Yakoby et al., 2008)

UAS-λ-topDER

(Flores et al., 2000)

UAS-λ^top

(Fernandes et al., 2017, Sudarsan et al., 2002)

UAS-λDER

(Li et al., 2009, Li and Carthew, 2005, Xu et al., 2000)

UAS-λEgfr

(Garcia-Alonso, 2024)

UAS-λTOP

(Jin et al., 2015, Jiang and Edgar, 2009, Luschnig et al., 2000)

UAS-λTop

(Yuen et al., 2022, Caviglia and Luschnig, 2013, Leatherbarrow and Halfon, 2009, Cabernard and Affolter, 2005)

UAS-λTop^4.2

(Giorgianni and Mann, 2011)

UAS-λtop^4.2

(Cordero et al., 2004, Wessells et al., 1999)

UAS-λtorpedo

(Sun et al., 2011, Lievens et al., 2005)

UAS:EGFR^gammatop

(Ewen-Campen and Perrimon, 2024)

UAS:EgfrλTop

(Rambur et al., 2020)

UASEgfr^λ4.2

(Galindo et al., 2002)

UAST-λtop

(Ghiglione et al., 2003)

activated-EGFR

(Kristiansen et al., 2005)

caEGFR

(Revaitis et al., 2017, Yakoby et al., 2008)

dEGFR^λ

(Park et al., 2017, Agnihotri et al., 2016, Read et al., 2009)

dEGFRλ

(Read et al., 2013)

λ-EGFR

(Wan et al., 2013)

λ-Top

(Zhu et al., 2005)

λ-Top-DA

(Richter et al., 2011)

λ-top^38B

(Wang and Pai, 2011)

λ-top^4.2

(Wang and Pai, 2011)

λEgfr

(Velasquez et al., 2022)

Secondary FlyBase IDs

References (160)

Report Sections