Recombinant construct P{UAS-tgo.DN}

General Information
Symbol	P{UAS-tgo.DN}	FlyBase ID	FBtp0008797
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Ohshiro and Saigo, 1997)
Uses	CV term Qualifiers & info Reference binary system (targeted) tgo (Ohshiro and Saigo, 1997) characterization tgo (Ohshiro and Saigo, 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Ohshiro and Saigo, 1997)
Component Alleles
Allele	tgo^DN.Scer\UAS
Reference(s)	(Ohshiro and Saigo, 1997, Umetsu et al., 2006, Schmidt et al., 2011)
Molecular data	Construct: A dominant negative form of tgo, in which a 13 amino acid basic region (SRENHCEIERRRR) is replaced by the sequence GIL is expressed under the control of four copies of the Scer\UAS regulatory sequence and a heat shock promoter. (Ohshiro and Saigo, 1997)
Phenotypic class	cell migration defective, with Scer\GAL4^loco.1.3 (Schmidt et al., 2011) neuroanatomy defective, with Scer\GAL4^loco.1.3 (Schmidt et al., 2011) neuroanatomy defective, with Scer\GAL4^NP6099 (Umetsu et al., 2006)
Phenotype manifest in	embryonic/larval brain, with Scer\GAL4^NP6099 (Umetsu et al., 2006) embryonic/larval tracheal system, with Scer\GAL4^btl.PS (Ohshiro and Saigo, 1997) exit glial cell, with Scer\GAL4^loco.1.3 (Schmidt et al., 2011) glial cell, with Scer\GAL4^loco.1.3 (Schmidt et al., 2011) lamina forming neuroblast, with Scer\GAL4^NP6099 (Umetsu et al., 2006)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-tgo.DN} (FlyBase, 1996-) Tango^DN (Schmidt et al., 2011) UAS-ARNT^DN (Umetsu et al., 2006)
Secondary FlyBase IDs

References ( 3 )
Research paper	Schmidt et al., 2011, Glia 59(9): 1264--1272 Transcriptional regulation of peripheral glial cell differentiation in the embryonic nervous system of drosophila. [FBrf0214270] Umetsu et al., 2006, Development 133(5): 791--800 The highly ordered assembly of retinal axons and their synaptic partners is regulated by Hedgehog/Single-minded in the Drosophila visual system. [FBrf0190327] Ohshiro and Saigo, 1997, Development 124(20): 3975--3986 Transcriptional regulation of breathless FGF receptor gene by binding of TRACHEALESS/dARNT heterodimers to three central midline elements in Drosophila developing trachea. [FBrf0099429]