Recombinant construct P{hhCD2}

General Information
Symbol	P{hhCD2}	FlyBase ID	FBtp0008937
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Strigini and Cohen, 1997)
Uses	CV term Qualifiers & info Reference binary system (targeted) hh (Strigini and Cohen, 1997) characterization hh (Strigini and Cohen, 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Strigini and Cohen, 1997) molecular tag (Strigini and Cohen, 1997)
Component Alleles
Allele	hh^{SF.Scer\UAS.T:Rnor\CD2}
Reference(s)	(Burke et al., 1999, Fujise et al., 2003, Chamoun et al., 2001, Gallet et al., 2006, Gallet et al., 2003, Denef et al., 2000, Tanimoto et al., 2000, Dominguez, 1999, Joulia et al., 2005, Strigini and Cohen, 1997, Casali, 2010)
Molecular data	Construct: hh signalling fragment is cloned in frame with the coding sequence of Rnor\CD2 (excluding the signal peptide and first amino acid) and tubulin 3' UTR. Fusion fragment is cloned into the pUAST vector. (Strigini and Cohen, 1997)
Phenotypic class
Phenotype manifest in	embryonic/first instar larval cuticle, with Scer\GAL4^en-e16E, hh^{C85S.Scer\UAS.cGa} (Gallet et al., 2006) embryonic/first instar larval cuticle, with Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}, hh^AC (Gallet et al., 2006) embryonic/larval epidermis, with Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}, hh^AC (Gallet et al., 2006)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	HhCD2 (Joulia et al., 2005) Hh-CD2 (Dominguez, 1999) P{hhCD2} (FlyBase, 1996-) UAS CD2-Hh (Casali, 2010) UAS-CD2-Hh (Casali, 2010) UAS-hhCD2 (Fujise et al., 2003, Tanimoto et al., 2000) UAS-Hh-CD2 (Denef et al., 2000) UAS-hh-CD2 (Burke et al., 1999) UAS-hh-N-CD2 (Gallet et al., 2003, Gallet et al., 2006)
Secondary FlyBase IDs

References ( 11 )
Research paper	Casali, 2010, Sci. Signal. 3(136): ra63 Self-induced patched receptor down-regulation modulates cell sensitivity to the hedgehog morphogen gradient. [FBrf0214124] Gallet et al., 2006, Development 133(3): 407--418 Cholesterol modification is necessary for controlled planar long-range activity of Hedgehog in Drosophila epithelia. [FBrf0190290] Joulia et al., 2005, Dev. Biol. 278(2): 496--510 Homeotic proboscipedia function modulates hedgehog-mediated organizer activity to pattern adult Drosophila mouthparts. [FBrf0183854] Fujise et al., 2003, Development 130(8): 1515--1522 Dally regulates Dpp morphogen gradient formation in the Drosophila wing. [FBrf0158874] Gallet et al., 2003, Dev. Cell 4(2): 191--204 Cholesterol modification of hedgehog is required for trafficking and movement, revealing an asymmetric cellular response to hedgehog. [FBrf0155685] Chamoun et al., 2001, Science 293(5537): 2080--2084 Skinny hedgehog, an acyltransferase required for palmitoylation and activity of the hedgehog signal. [FBrf0138534] Denef et al., 2000, Cell 102(4): 521--531 Hedgehog induces opposite changes in turnover and subcellular localization of Patched and Smoothened. [FBrf0129789] Tanimoto et al., 2000, Mol. Cell 5(1): 59--71 Hedgehog creates a gradient of DPP activity in Drosophila wing imaginal discs. [FBrf0125394] Burke et al., 1999, Cell 99(7): 803--815 Dispatched, a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells. [FBrf0122981] Dominguez, 1999, Development 126(11): 2345--2353 Dual role for Hedgehog in the regulation of the proneural gene atonal during ommatidia development. [FBrf0108513] Strigini and Cohen, 1997, Development 124(22): 4697--4705 A Hedgehog activity gradient contributes to AP axial patterning of the Drosophila wing. [FBrf0099736]