Recombinant construct P{UAS-Ras85D.V12.C40}

General Information
Symbol	P{UAS-Ras85D.V12.C40}	FlyBase ID	FBtp0009106
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Karim and Rubin, 1998)
Uses	CV term Qualifiers & info Reference binary system (targeted) Ras85D (Karim and Rubin, 1998) characterization Ras85D (Karim and Rubin, 1998)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Karim and Rubin, 1998)
Component Alleles
Allele	Ras85D^{V12.C40.Scer\UAS}
Reference(s)	(Karim and Rubin, 1998, Botella et al., 2003, Asha et al., 2003, Koh et al., 2002, Prober and Edgar, 2002, Gaengel and Mlodzik, 2003, Therrien et al., 1999, Bergmann et al., 1998, Yang and Baker, 2003, Mirey et al., 2003, Yan et al., 2009, Freeman et al., 2010)
Molecular data	Construct: Amino acid replacement: Y40C. An effector loop mutant. (Karim and Rubin, 1998)
Phenotypic class	cell polarity defective, with Scer\GAL4^HLHmδ.0.5 (Gaengel and Mlodzik, 2003) cell polarity defective, with Scer\GAL4^hs.2sev (Gaengel and Mlodzik, 2003) hyperplasia, with Scer\GAL4^dpp.blk1 (Karim and Rubin, 1998) neuroanatomy defective, with Scer\GAL4^futsch-C380 (Koh et al., 2002) viable, with Scer\GAL4^elav-C155 (Botella et al., 2003) visible, with Scer\GAL4^dpp.blk1 (Karim and Rubin, 1998) visible, with Scer\GAL4^GMR.PF (Mirey et al., 2003) visible, with Scer\GAL4^hs.2sev (Therrien et al., 1999)
Phenotype manifest in	bouton \| supernumerary, with Scer\GAL4^futsch-C380 (Koh et al., 2002) eye, with Scer\GAL4^GMR.PF (Mirey et al., 2003) eye, with Scer\GAL4^hs.2sev (Therrien et al., 1999) ommatidium, with Scer\GAL4^HLHmδ.0.5 (Gaengel and Mlodzik, 2003) ommatidium, with Scer\GAL4^hs.2sev (Gaengel and Mlodzik, 2003) wing, with Scer\GAL4^dpp.blk1 (Karim and Rubin, 1998) wing disc, with Scer\GAL4^dpp.blk1 (Karim and Rubin, 1998) wing vein, with Scer\GAL4^dpp.blk1 (Karim and Rubin, 1998)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[UAS-Ras1^V12C40] (Botella et al., 2003) P{UAS-Ras85D.V12.C40} (FlyBase, 1996-) UAS:Ras1^V12C40 (Yang and Baker, 2003) UAS-RAS1^V12C40 (Therrien et al., 1999) UAS-Ras1<up>V12 C40</up> (Mirey et al., 2003) UAS-Ras1V12C40 (Koh et al., 2002) UAS-Ras185DV12C40 (Freeman et al., 2010) UAS-Ras^V12C40 (Prober and Edgar, 2002) UAS-RasV12,C40 (Asha et al., 2003) UAS-RasV12C40 (Yan et al., 2009)
Secondary FlyBase IDs

References ( 12 )
Research paper	Freeman et al., 2010, Brain Res. 1326: 15--29 A new genetic model of activity-induced Ras signaling dependent pre-synaptic plasticity in Drosophila. [FBrf0210453] Yan et al., 2009, J. Cell Sci. 122(9): 1461--1470 Impairment of ubiquitylation by mutation in Drosophila E1 promotes both cell-autonomous and non-cell-autonomous Ras-ERK activation in vivo. [FBrf0207806] Asha et al., 2003, Genetics 163(1): 203--215 Analysis of ras-induced overproliferation in Drosophila hemocytes. [FBrf0155813] Botella et al., 2003, Mol. Biol. Cell 14(1): 241--250 Deregulation of the Egfr/Ras signaling pathway induces age-related brain degeneration in the Drosophila mutant vap. [FBrf0155942] Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423 Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499] Mirey et al., 2003, Mol. Cell. Biol. 23(3): 1112--1124 A Ral Guanine Exchange Factor-Ral Pathway Is Conserved in Drosophila melanogaster and Sheds New Light on the Connectivity of the Ral, Ras, and Rap Pathways. [FBrf0155960] Yang and Baker, 2003, Dev. Cell 4(3): 359--369 Cell cycle withdrawal, progression, and cell survival regulation by EGFR and its effectors in the differentiating Drosophila eye. [FBrf0158858] Koh et al., 2002, J. Neurosci. 22(7): 2496--2504 The Ras1-mitogen-activated protein kinase signal transduction pathway regulates synaptic plasticity through fasciclin II-mediated cell adhesion. [FBrf0151505] Prober and Edgar, 2002, Genes Dev. 16(17): 2286--2299 Interactions between Ras1, dMyc, and dPI3K signaling in the developing Drosophila wing. [FBrf0151998] Therrien et al., 1999, Proc. Natl. Acad. Sci. U.S.A. 96(23): 13259--13263 Functional analysis of CNK in RAS signaling. [FBrf0112056] Bergmann et al., 1998, Cell 95(3): 331--341 The Drosophila gene hid is a direct molecular target of Ras-dependent survival signaling. [FBrf0105197] Karim and Rubin, 1998, Development 125(1): 1--9 Ectopic expression of activated Ras1 induces hyperplastic growth and increased cell death in Drosophila imaginal tissues. [FBrf0100097]