Recombinant construct P{nub-GAL4.K}

General Information
Symbol	P{nub-GAL4.K}	FlyBase ID	FBtp0009119
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Kambadur et al., 1998)
Uses	CV term Qualifiers & info Reference binary system (regulatory) nub (Kambadur et al., 1998)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Kambadur et al., 1998)
Component Alleles
Allele	Scer\GAL4^nub.PK
Reference(s)	(Kambadur et al., 1998, Hsu et al., 2007, Kuzin et al., 2005, Koizumi et al., 2001, Thompson and Cohen, 2006, Baena-Lopez and Garcia-Bellido, 2006, Tien et al., 2008)
Molecular data	Construct: 5.3kb EcoRI fragment of nub promoter region drives expression of a 2.8kb KpnI-NotI fragment of Scer\GAL4. (Kambadur et al., 1998)
Phenotypic class	cytokinesis defective, with vg^Scer\UAS.cZa (Baena-Lopez and Garcia-Bellido, 2006) decreased cell number, with Tctp^{dsRNA.Scer\UAS} (Hsu et al., 2007) decreased cell size, with Tctp^{dsRNA.Scer\UAS} (Hsu et al., 2007) lethal \| larval stage, with Ran^{G19V.Q69L.Scer\UAS} (Koizumi et al., 2001) size defective, with vg^Scer\UAS.cKa (Baena-Lopez and Garcia-Bellido, 2006) viable, with Ran^Scer\UAS.cKa (Koizumi et al., 2001) visible, with Tctp^{dsRNA.Scer\UAS} (Hsu et al., 2007)
Phenotype manifest in	wing, with Tctp^{dsRNA.Scer\UAS} (Hsu et al., 2007) wing, with vg^Scer\UAS.cKa (Baena-Lopez and Garcia-Bellido, 2006) wing disc, with vg^Scer\UAS.cZa (Baena-Lopez and Garcia-Bellido, 2006)
Other information

Allele	w^nub.PK
Reference(s)	(Kambadur et al., 1998)
Molecular data	Construct: 5.3kb EcoRI fragment of nub promoter region influences expression of w^+mC. (Kambadur et al., 1998)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference embryonic stage ganglion mother cell (Kuzin et al., 2005) neuron (Kuzin et al., 2005) neuroblast (Kuzin et al., 2005, Koizumi et al., 2001) embryonic stage 9 -- 10 ventral nerve cord primordium \| restricted (Kambadur et al., 1998) cell of ventral nerve cord primordium (Kambadur et al., 1998) embryonic stage 10 -- 12 neuroblast \| subset (Kambadur et al., 1998) ganglion mother cell \| subset (Kambadur et al., 1998)
Additional Information
Statement Reference Expression pattern is observed to be the same as the pattern of endogenous nub expression. Expression is not observed in fully delaminated neuroblasts at embryonic stage 9. At embryonic stage 10, expression is also seen in a small subset of ventral nerve cord neuroblasts and ganglion mother cells. Late in embryonic stage 10 and into stage 11, expression is observed in ganglion mother cells and many neuroblasts of the cephalic lobe and the ventral nerve cord. The intensity of neuroblast expression decreases during stage 12. (Kambadur et al., 1998)

Marker for	neuroectoderm (Kambadur et al., 1998) neuroblast ganglion mother cell neuron (Kuzin et al., 2005) neuroectoderm neuroblast ganglion mother cell (Kambadur et al., 1998) ganglion mother cell (Kambadur et al., 1998) neuroblast (Koizumi et al., 2001)
Reflects expression of	nub (Kuzin et al., 2005, Koizumi et al., 2001, Kambadur et al., 1998)
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	42699 w^*; P{nub-GAL4.K}2; P{GD3577}v51169
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	nubbin-G4 (Baena-Lopez and Garcia-Bellido, 2006) nubbin-Gal4 (Thompson and Cohen, 2006) nubG4 (Baena-Lopez and Garcia-Bellido, 2006) nubG4^K (Baena-Lopez and Garcia-Bellido, 2006) nub-GAL4 (Tien et al., 2008, Hsu et al., 2007) P{nub-GAL4.K} (FlyBase, 1996-) pdm-1-GAL4 (Kuzin et al., 2005) pdm.Gal4 (Koizumi et al., 2001)
Secondary FlyBase IDs

References ( 8 )
Research paper	Tien et al., 2008, J. Cell Biol. 182(6): 1113--1125 Ero1L, a thiol oxidase, is required for Notch signaling through cysteine bridge formation of the Lin12-Notch repeats in Drosophila melanogaster. [FBrf0206028] Hsu et al., 2007, Nature 445(7129): 785--788 Drosophila TCTP is essential for growth and proliferation through regulation of dRheb GTPase. [FBrf0192022] Baena-Lopez and Garcia-Bellido, 2006, Proc. Natl. Acad. Sci. U.S.A. 103(37): 13734--13739 Control of growth and positional information by the graded vestigial expression pattern in the wing of Drosophila melanogaster. [FBrf0193315] Thompson and Cohen, 2006, Cell 126(4): 767--774 The hippo pathway regulates the bantam microRNA to control cell proliferation and apoptosis in Drosophila. [FBrf0193861] Koizumi et al., 2001, Dev. Genes Evol. 211(2): 67--75 A search for Drosophila neural precursor genes identifies ran. [FBrf0135723] Kambadur et al., 1998, Genes Dev. 12(2): 246--260 Regulation of POU genes by castor and hunchback establishes layered compartments in the Drosophila CNS. [FBrf0100094]
Supplementary material	Kuzin et al., 2005, Dev. Biol. 277(2): Supplemental materials. [FBrf0183076]
Personal communication to FlyBase	Bellen, 2012.12.5, Bellen insertions. Bellen insertions. [FBrf0220067]