Recombinant construct P{UAS-robo.K}

General Information
Symbol	P{UAS-robo.K}	FlyBase ID	FBtp0009131
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Kidd et al., 1998)
Uses	CV term Qualifiers & info Reference binary system (targeted) robo (Kidd et al., 1998) characterization robo (Kidd et al., 1998)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Kidd et al., 1998) selectable marker (Kidd et al., 1998)
Component Alleles
Allele	robo^Scer\UAS.cKa
Reference(s)	(Bhat, 2005, Allan et al., 2003, Godenschwege et al., 2002, Wills et al., 2002, Englund et al., 2002, Couch et al., 2004, Jhaveri et al., 2004, Simpson et al., 2000, Kinrade et al., 2001, Simpson et al., 2000, Bashaw et al., 2000, Kidd et al., 1999, Bashaw and Goodman, 1999, Kramer et al., 2001, Kramer et al., 2001, Kidd et al., 1998, Santiago-Martinez et al., 2006, Mauss et al., 2009, Deng et al., 2010)
Molecular data	Construct: A robo cDNA containing the entire open reading frame but lacking most of the untranslated regions is expressed under the control of Scer\UAS regulatory sequences. (Kidd et al., 1998)
Phenotypic class	neuroanatomy defective, with Scer\GAL4^A307 (Godenschwege et al., 2002) neuroanatomy defective, with Scer\GAL4^A307, Scer\GAL4^{shakB.lethal.4.1} (Godenschwege et al., 2002) neuroanatomy defective, with Scer\GAL4^c17 (Godenschwege et al., 2002) neuroanatomy defective, with Scer\GAL4^elav.PLu (Bhat, 2005) neuroanatomy defective, with Scer\GAL4^elav-C155 (Wills et al., 2002) neuroanatomy defective, with Scer\GAL4^eve.CQ2 (Mauss et al., 2009) neuroanatomy defective, with Scer\GAL4^{shakB.lethal.4.1} (Godenschwege et al., 2002)
Phenotype manifest in	A1-7 lateral transverse muscle 1, with Scer\GAL4^how-24B (Kramer et al., 2001, Kramer et al., 2001) A1-7 lateral transverse muscle 2, with Scer\GAL4^how-24B (Kramer et al., 2001, Kramer et al., 2001) A1-7 lateral transverse muscle 3, with Scer\GAL4^how-24B (Kramer et al., 2001, Kramer et al., 2001) antennal commissure, with Scer\GAL4^lz-gal4 (Jhaveri et al., 2004) antennal glomerulus DM2 & olfactory neuron, with Scer\GAL4^lz-gal4 (Jhaveri et al., 2004) antennal glomerulus DM6 & olfactory neuron, with Scer\GAL4^lz-gal4 (Jhaveri et al., 2004) antennal lobe glomerulus, with Scer\GAL4^SG18.1 (Jhaveri et al., 2004) commissure, with Scer\GAL4^elav.PLu (Bashaw et al., 2000) dendrite, with Scer\GAL4^eve.CQ2 (Mauss et al., 2009) dMP2 neuron, with Scer\GAL4^15J2 (Simpson et al., 2000) embryonic/larval neuron, with Scer\GAL4^ap-md544 (Allan et al., 2003) ganglionic branch primordium, with Scer\GAL4^bs.Term (Englund et al., 2002) giant fiber neuron, with Scer\GAL4^A307 (Godenschwege et al., 2002) giant fiber neuron, with Scer\GAL4^c17 (Godenschwege et al., 2002) intersegmental nerve, with Scer\GAL4^elav-C155 (Wills et al., 2002) longitudinal connective, with Scer\GAL4^elav.PLu (Bhat, 2005) longitudinal connective, with Scer\GAL4^s.gcm, robo³ (Kinrade et al., 2001) motor neuron, with Scer\GAL4^eve.CQ2 (Mauss et al., 2009) presumptive embryonic/larval central nervous system, with Scer\GAL4^elav.PLu (Kidd et al., 1999) synapse \| adult stage, with Scer\GAL4^A307 (Godenschwege et al., 2002) synapse \| adult stage, with Scer\GAL4^A307, Scer\GAL4^{shakB.lethal.4.1} (Godenschwege et al., 2002) synapse \| adult stage, with Scer\GAL4^c17 (Godenschwege et al., 2002) synapse \| adult stage, with Scer\GAL4^{shakB.lethal.4.1} (Godenschwege et al., 2002) tergal depressor of trochanter muscle motor neuron & dendrite, with Scer\GAL4^{shakB.lethal.4.1} (Godenschwege et al., 2002) ventral nerve cord, with Scer\GAL4^ap-md544 (Allan et al., 2003) ventral nerve cord commissure, with Scer\GAL4^elav.PLu (Simpson et al., 2000) vMP2 neuron, with Scer\GAL4^15J2 (Simpson et al., 2000)
Other information

Allele	w^+mC
Reference(s)	(Kidd et al., 1998)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-robo.K} (FlyBase, 1996-) UAS-robo (Bhat, 2005, Couch et al., 2004, Jhaveri et al., 2004, Allan et al., 2003, Englund et al., 2002, Godenschwege et al., 2002, Kramer et al., 2001, Kramer et al., 2001, Simpson et al., 2000, Simpson et al., 2000, Bashaw and Goodman, 1999, Santiago-Martinez et al., 2006, Mauss et al., 2009, Deng et al., 2010) UAS-Robo (Wills et al., 2002) UAS robo (Kinrade et al., 2001) UASrobo (Bashaw et al., 2000)
Secondary FlyBase IDs

References ( 19 )
Research paper	Deng et al., 2010, Mol. Biol. Cell 21(19): 3304--3316 Vestigial is required during late-stage muscle differentiation in Drosophila melanogaster embryos. [FBrf0211962] Mauss et al., 2009, PLoS Biol. 7(9): e1000200 Midline signalling systems direct the formation of a neural map by dendritic targeting in the Drosophila motor system. [FBrf0208790] Santiago-Martinez et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(33): 12441--12446 Lateral positioning at the dorsal midline: Slit and Roundabout receptors guide Drosophila heart cell migration. [FBrf0194038] Bhat, 2005, Genetics 170(1): 149--159 Slit-roundabout signaling neutralizes Netrin-Frazzled-mediated attractant cue to specify the lateral positioning of longitudinal axon pathways. [FBrf0187630] Couch et al., 2004, Development 131(5): 997--1006 robo2 and robo3 interact with eagle to regulate serotonergic neuron differentiation. [FBrf0174592] Jhaveri et al., 2004, Development 131(9): 1903--1912 Positioning sensory terminals in the olfactory lobe of Drosophila by Robo signaling. [FBrf0174568] Englund et al., 2002, Development 129(21): 4941--4951 Attractive and repulsive functions of Slit are mediated by different receptors in the Drosophila trachea. [FBrf0151931] Godenschwege et al., 2002, J. Neurosci. 22(8): 3117--3129 Ectopic expression in the giant fiber system of Drosophila reveals distinct roles for roundabout (Robo), Robo2, and Robo3 in dendritic guidance and synaptic connectivity. [FBrf0147116] Wills et al., 2002, Neuron 36(4): 611--622 A Drosophila homolog of cyclase-associated proteins collaborates with the abl tyrosine kinase to control midline axon pathfinding. [FBrf0152273] Kinrade et al., 2001, Development 128(2): 207--216 Roundabout signalling, cell contact and trophic support confine longitudinal glia and axons in the Drosophila CNS. [FBrf0132293] Kramer et al., 2001, Science 292(5517): 737--740 Switching repulsion to attraction: changing responses to slit during transition in mesoderm migration. [FBrf0135949] Bashaw et al., 2000, Cell 101(7): 703--715 Repulsive axon guidance: abelson and enabled play opposing roles downstream of the roundabout receptor. [FBrf0128391] Simpson et al., 2000, Neuron 28(3): 753--766 Short-range and long-range guidance by Slit and its Robo receptors: robo and Robo2 play distinct roles in midline guidance. [FBrf0132431] Simpson et al., 2000, Cell 103(7): 1019--1032 Short-range and long-range guidance by Slit and its Robo receptors: a combinatorial code of Robo receptors controls lateral position. [FBrf0132257] Bashaw and Goodman, 1999, Cell 97(7): 917--926 Chimeric axon guidance receptors: the cytoplasmic domains of slit and netrin receptors specify attraction versus repulsion. [FBrf0108593] Kidd et al., 1999, Cell 96(6): 785--794 Slit is the midline repellent for the robo receptor in Drosophila. [FBrf0107807] Kidd et al., 1998, Cell 92(2): 205--215 Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors. [FBrf0100585]
Supplementary material	Allan et al., 2003, Cell 113(1): Supplementary material: tables S1 - S5. [FBrf0161463] Kramer et al., 2001, Science 292(5517): Switching repulsion to attraction: changing responses to slit during transition in mesoderm migration. [FBrf0136716]