Recombinant construct P{αTub84B(FRT.y⁺)pb.Q50K}

General Information
Symbol	P{αTub84B(FRT.y⁺)pb.Q50K}	FlyBase ID	FBtp0009134
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Percival-Smith et al., 1997)
Uses	CV term Qualifiers & info Reference characterization pb \| mosaic analysis (Percival-Smith et al., 1997) FRT construct pb (Percival-Smith et al., 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference general promoter (Percival-Smith et al., 1997) removable cassette (Percival-Smith et al., 1997) selectable marker (Percival-Smith et al., 1997) selectable marker (Percival-Smith et al., 1997)
Component Alleles
Allele	pb^{Q50K.Scer\FRT.αTub84B}
Reference(s)	(Percival-Smith et al., 1997)
Molecular data	Construct: Amino acid replacement: Q50K. 2.6kb EcoRI-KpnI fragment carrying αTub84B promoter is separated from the mutant pb coding region by an Scer\FRT cassette carrying a transcriptional termination region and y^+t7.7. (Percival-Smith et al., 1997)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Percival-Smith et al., 1997)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information

Allele	y^+t7.7
Reference(s)	(Percival-Smith et al., 1997)
Molecular data	Construct: 8kb SalI fragment carrying y⁺. (Merriam, 1997.5.9) Contained within 7.7kb SalI-BglII fragment that includes the entire y⁺ gene. (Struhl and Basler, 1993) Fragment containing the y gene, including 2873bp 5' and 130bp 3' flanking sequences. (Harrison et al., 1989) 5' regulatory region deletion derivative of y, 2873 nucleotides remain upstream of the transcription start site. (Geyer and Corces, 1987)
Phenotypic class	wild-type (Benedyk et al., 1994, Geyer and Corces, 1987, Harrison et al., 1989)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)	P{αTub84B(-FRT)pb.Q50K} (Percival-Smith et al., 1997)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{pb.FRT.Q50K} (FlyBase, 1996-) P{αTub84B(FRT.y⁺)pb.Q50K} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Percival-Smith et al., 1997, Development 124(24): 5049--5062 Genetic characterization of the role of the two HOX proteins, Proboscipedia and Sex Combs Reduced, in determination of adult antennal, tarsal, maxillary palp and proboscis identities in Drosophila melanogaster. [FBrf0100199]

Recombinant construct P{αTub84B(FRT.y+)pb.Q50K}

Recombinant construct P{αTub84B(FRT.y⁺)pb.Q50K}