Recombinant construct P{UAS-N.ICN}

General Information
Symbol	P{UAS-N.ICN}	FlyBase ID	FBtp0009581
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Go et al., 1998)
Uses	CV term Qualifiers & info Reference binary system (targeted) N (Go et al., 1998) characterization N (Go et al., 1998)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Go et al., 1998) selectable marker (Go et al., 1998)
Component Alleles
Allele	N^ICN.Scer\UAS
Reference(s)	(Go et al., 1998, Reynolds-Kenneally and Mlodzik, 2005, Chern and Choi, 2002, Schober et al., 2005, Bataille et al., 2005, Matsuno et al., 2002, Lebestky et al., 2003, Takashima et al., 2002, Dutta et al., 2004, Chao et al., 2004, Brumby and Richardson, 2003, Yan et al., 2004, Yan et al., 2009, Brodu et al., 2004, Moon et al., 2005, Steneberg et al., 1999, Kurata et al., 2000, Wang et al., 2006, Mandal et al., 2004, Hori et al., 2004, Sasamura et al., 2003, Ghabrial and Krasnow, 2006, Bernard et al., 2006, Thomas and van Meyel, 2007, Hagedorn et al., 2006, Yamakawa et al., 2012, Domanitskaya and Schüpbach, 2012, Sun et al., 2011, Yan et al., 2009, Bernard et al., 2009, Wang et al., 2008, Bayraktar et al., 2006, Poulton et al., 2011, Olguín et al., 2011, Nfonsam et al., 2012)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of a constitutively active form of N in which the extracellular domain including the signal peptide and transmembrane domain is deleted. (Go et al., 1998)
Phenotypic class	hyperplasia, with Scer\GAL4^ey.PU (Bayraktar et al., 2006) hyperplasia \| cell non-autonomous \| somatic clone, with Scer\GAL4^Act5C.PI (Chao et al., 2004) hyperplasia \| somatic clone, with Scer\GAL4^Act5C.PP (Reynolds-Kenneally and Mlodzik, 2005) increased cell death \| somatic clone \| wandering third instar larval stage, with Scer\GAL4^Act5C.PI (Moon et al., 2005) lethal, with Scer\GAL4^ey.PH (Kurata et al., 2000) neuroanatomy defective \| somatic clone, with Scer\GAL4^Act5C.PI (Wang et al., 2006) visible, with Scer\GAL4^T113 (Go et al., 1998)
Phenotype manifest in	adult head \| somatic clone, with Scer\GAL4^Act5C.PP (Reynolds-Kenneally and Mlodzik, 2005) C1 chordotonal organ precursor cell, with Scer\GAL4^en-e16E (Brodu et al., 2004) crystal cell \| ectopic, with Scer\GAL4^hs.PB (Lebestky et al., 2003) embryonic/larval dorsal branch, with Scer\GAL4^btl.PS (Ghabrial and Krasnow, 2006) embryonic/larval lymph gland, with Scer\GAL4^hs.PB (Lebestky et al., 2003) embryonic/larval lymph gland, with Scer\GAL4^Mef2.PR (Mandal et al., 2004) embryonic/larval oenocyte precursor, with Scer\GAL4^en-e16E (Brodu et al., 2004) embryonic/larval trachea, with Scer\GAL4^btl.PS (Steneberg et al., 1999) embryonic/larval trachea, with Scer\GAL4^esg-p127 (Steneberg et al., 1999) embryonic hindgut, with Scer\GAL4^byn-Gal4 (Takashima et al., 2002) embryonic large intestine, with Scer\GAL4^byn-Gal4 (Takashima et al., 2002) eye, with Scer\GAL4^ey.PH (Kurata et al., 2000) eye, with Scer\GAL4^T113 (Go et al., 1998) eye \| ectopic, with Scer\GAL4^ey.PH (Kurata et al., 2000) eye-antennal disc \| somatic clone, with Scer\GAL4^Act5C.PP (Reynolds-Kenneally and Mlodzik, 2005) eye disc, with Scer\GAL4^dpp.blk1 (Chern and Choi, 2002) eye disc, with Scer\GAL4^ey.PU (Bayraktar et al., 2006) eye disc \| somatic clone, with Scer\GAL4^Act5C.PI (Chao et al., 2004) eye photoreceptor cell, with Scer\GAL4^ey.PH (Lee et al., 2009) myoblast \| P-stage, with Scer\GAL4¹¹⁵¹ (Dutta et al., 2004) neuroblast \| supernumerary & larval brain \| somatic clone, with Scer\GAL4^Act5C.PI (Wang et al., 2006) oenocyte primordium, with Scer\GAL4^en-e16E (Brodu et al., 2004) ommatidium, with Scer\GAL4^ey.PH (Kurata et al., 2000) ommatidium \| ectopic, with Scer\GAL4^ey.PH (Kurata et al., 2000) photoreceptor cell \| ectopic, with Scer\GAL4^ey.PH (Kurata et al., 2000) sensory mother cell \| ectopic, with Scer\GAL4^ptc-559.1 (Matsuno et al., 2002) ventral thoracic disc, with Scer\GAL4^T113 (Go et al., 1998) wing disc, with Scer\GAL4^30A (Go et al., 1998) wing disc, with Scer\GAL4^71B (Go et al., 1998) wing disc, with Scer\GAL4^A9 (Go et al., 1998) wing disc, with Scer\GAL4^ap-md544 (Nfonsam et al., 2012) wing disc, with Scer\GAL4^c766 (Go et al., 1998) wing disc, with Scer\GAL4^dpp.blk1 (Go et al., 1998) wing disc, with Scer\GAL4^T113 (Go et al., 1998) wing disc, with Scer\GAL4^vg.int2.1 (Go et al., 1998) wing disc \| somatic clone \| wandering third instar larval stage, with Scer\GAL4^Act5C.PI (Moon et al., 2005)
Other information

Allele	w^+mC
Reference(s)	(Go et al., 1998)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-N.ICN} (FlyBase, 1996-) UAS-activated Notch (Dutta et al., 2004) UAS-actN (Mandal et al., 2004) UAS-actNotch (Wang et al., 2006) UAS-N.ICN (Nfonsam et al., 2012) UAS-N^act (Reynolds-Kenneally and Mlodzik, 2005, Chao et al., 2004, Sasamura et al., 2003, Lebestky et al., 2003, Matsuno et al., 2002, Kurata et al., 2000, Wang et al., 2006, Wang et al., 2008) UAS-N^ACT (Ghabrial and Krasnow, 2006) UAS-Nⁱ (Yan et al., 2004, Yan et al., 2009) UAS-N^I (Bernard et al., 2006, Bernard et al., 2009) UAS-N^ICD (Hori et al., 2004, Thomas and van Meyel, 2007) UAS-N^intra (Mandal et al., 2004, Brumby and Richardson, 2003, Takashima et al., 2002, Chern and Choi, 2002, Steneberg et al., 1999, Brodu et al., 2004, Bayraktar et al., 2006) UAS-Nic (Moon et al., 2005) UAS-NICD (Yamakawa et al., 2012, Domanitskaya and Schüpbach, 2012, Yan et al., 2009, Poulton et al., 2011, Sun et al., 2011) uas-Nintra (Bataille et al., 2005) UAS-Notch intracellular domain (Poulton et al., 2011) unnamed (Hagedorn et al., 2006)
Secondary FlyBase IDs

References ( 35 )

Generate a list of	All references relating to this transgenic transposon ( 35 )

List References by type	Research paper ( 35 )
Recent research papers ( 6 )
	Domanitskaya and Schüpbach, 2012, J. Cell Sci. 125(2): 399--410 CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster. [FBrf0217558] Nfonsam et al., 2012, PLoS ONE 7(8): e44583 Analysis of the Transcriptomes Downstream of Eyeless and the Hedgehog, Decapentaplegic and Notch Signaling Pathways in Drosophila melanogaster. [FBrf0219414] Yamakawa et al., 2012, Development 139(3): 558--567 Deficient Notch signaling associated with neurogenic pecanex is compensated for by the unfolded protein response in Drosophila. [FBrf0217160] Olguín et al., 2011, Curr. Biol. 21(3): 236--242 Intertissue mechanical stress affects frizzled-mediated planar cell polarity in the Drosophila notum epidermis. [FBrf0212947] Poulton et al., 2011, Development 138(9): 1737--1745 The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. [FBrf0213494] Sun et al., 2011, Development 138(10): 1991--2001 Regulation of somatic myosin activity by Protein Phosphatase 1{beta} controls Drosophila oocyte polarization. [FBrf0213586] Show all research papers ...