Recombinant construct P{UAS-Hsap\MJD.tr-Q78}

General Information
Symbol	P{UAS-Hsap\MJD.tr-Q78}	FlyBase ID	FBtp0009597
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms decreased cell number somatic clone anterior wing margin sensory neuron
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Warrick et al., 1998)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Warrick et al., 1998) selectable marker (Warrick et al., 1998)
Component Alleles
Allele	Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}
Reference(s)	(Martin-Lanneree et al., 2006, Warrick et al., 1999, Mizutani et al., 2005, Jung and Bonini, 2007, Moreno et al., 2002, Gunawardena et al., 2003, Lee et al., 2004, Bilen et al., 2006, Kanuka et al., 2003, Perez et al., 1998, Bonini, 1999, Warrick et al., 1998, Senoo-Matsuda et al., 2005, Kadener et al., 2006, Boeddrich et al., 2006, Umeda-Kameyama et al., 2007, Warrick et al., 2005, Bonini, 2002, Bohm et al., 2010, Ryoo et al., 2007, Mallik and Lakhotia, 2009, Arya et al., 2010, Khurana et al., 2006, Mallik and Lakhotia, 2010, Jung et al., 2009, Vos et al., 2010, Carra et al., 2010, Watson et al., 2008, Park et al., 2011, Ghosh and Feany, 2004, Khurana et al., 2010, Chang et al., 2011, Tonoki et al., 2011, Butler et al., 2012, Liu et al., 2012, Lessing and Bonini, 2008, Bhutani et al., 2012, Chan et al., 2000, Fujikake et al., 2008, Bilen and Bonini, 2007, Li et al., 2008)
Molecular data	Construct: A truncated form of Hsap\MJD, consisting of an expanded length glutamine tract (78 repeats) flanked upstream by 12 amino acids and downstream by the carboxy terminal 43 amino acids of Hsap\MJD, is expressed under the control of Scer\UAS regulatory sequences. The Hsap\MJD protein is tagged at the N-terminal end with T:Ivir\HA1. (Warrick et al., 1998)
Phenotypic class	courtship behavior defective \| female, with Scer\GAL4^Ddc.PL (Kadener et al., 2006) decreased cell number \| conditional, with Scer\GAL4^ninaE.PD (Lessing and Bonini, 2008) decreased cell number \| somatic clone, with Scer\GAL4^elav-C155 (Lessing and Bonini, 2008) eye color defective, with Scer\GAL4^GMR.PF (Mallik and Lakhotia, 2009) gravitaxis defective \| progressive, with Scer\GAL4^elav-C155 (Li et al., 2008) increased cell death, with Scer\GAL4^30Y (Khurana et al., 2006) increased cell death, with Scer\GAL4^179Y (Gunawardena et al., 2003) increased cell death, with Scer\GAL4^Appl.G1a (Gunawardena et al., 2003) increased cell death, with Scer\GAL4^GMR.PF (Moreno et al., 2002, Bilen et al., 2006) lethal, with Scer\GAL4^elav-C155 (Warrick et al., 1998, Warrick et al., 1999, Chan et al., 2000) lethal, with Scer\GAL4^how-24B (Warrick et al., 1998) lethal \| heat sensitive, with Scer\GAL4^179Y (Gunawardena et al., 2003) lethal \| heat sensitive, with Scer\GAL4^Appl.G1a (Gunawardena et al., 2003) lethal \| pharate adult stage, with Scer\GAL4^elav-C155 (Mallik and Lakhotia, 2009) lethal \| P-stage, with Scer\GAL4^elav.PU (Arya et al., 2010) locomotor behavior defective, with Scer\GAL4^elav.PLu (Umeda-Kameyama et al., 2007) locomotor behavior defective \| larval stage, with Scer\GAL4^179Y (Gunawardena et al., 2003) locomotor behavior defective \| larval stage, with Scer\GAL4^Appl.G1a (Gunawardena et al., 2003) locomotor rhythm defective, with Scer\GAL4^tim.PE (Kadener et al., 2006) mating defective, with Scer\GAL4^tim.PE (Kadener et al., 2006) neuroanatomy defective (Boeddrich et al., 2006) neuroanatomy defective, with Scer\GAL4^elav-C155 (Khurana et al., 2010) neuroanatomy defective, with Scer\GAL4^GMR.PU (Chan et al., 2000) neuroanatomy defective \| adult stage, with Scer\GAL4^GMR.PF (Fujikake et al., 2008) neuroanatomy defective \| adult stage, with Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) neuroanatomy defective \| conditional, with Scer\GAL4^ninaE.PT (Liu et al., 2012) neuroanatomy defective \| progressive, with Scer\GAL4^GMR.PU (Chan et al., 2000) partially lethal - majority die, with Scer\GAL4^elav.PLu (Fujikake et al., 2008) phototaxis defective, with Scer\GAL4^GMR.PF (Mallik and Lakhotia, 2009) phototaxis defective, with Scer\GAL4^GMR.PU (Arya et al., 2010) phototaxis defective \| adult stage, with Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) short lived, with Scer\GAL4^elav-C155 (Senoo-Matsuda et al., 2005, Li et al., 2008) short lived, with Scer\GAL4^tim.PE (Kadener et al., 2006) short lived \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Martin-Lanneree et al., 2006) visible, with Scer\GAL4^elav-C155 (Warrick et al., 1998) visible, with Scer\GAL4^GMR.long (Butler et al., 2012) visible, with Scer\GAL4^GMR.PF (Warrick et al., 1998, Martin-Lanneree et al., 2006, Warrick et al., 1999, Bonini, 1999, Warrick et al., 2005, Bonini, 2002, Bohm et al., 2010) visible, with Scer\GAL4^GMR.PU (Arya et al., 2010, Chan et al., 2000, Bilen and Bonini, 2007) visible \| adult stage, with Scer\GAL4^GMR.PF (Fujikake et al., 2008)
Phenotype manifest in	adult brain, with Scer\GAL4^30Y (Khurana et al., 2006, Ghosh and Feany, 2004) adult brain, with Scer\GAL4^elav-C155 (Warrick et al., 1998) adult brain \| conditional, with Scer\GAL4^ninaE.PT (Liu et al., 2012) anterior wing margin \| somatic clone, with Scer\GAL4^elav-C155 (Lessing and Bonini, 2008) eye, with Scer\GAL4^elav-C155 (Warrick et al., 1998, Warrick et al., 1999) eye, with Scer\GAL4^GMR.long (Butler et al., 2012) eye, with Scer\GAL4^GMR.PF (Moreno et al., 2002, Warrick et al., 1998, Mizutani et al., 2005, Martin-Lanneree et al., 2006, Kanuka et al., 2003, Warrick et al., 1999, Bonini, 1999, Senoo-Matsuda et al., 2005, Warrick et al., 2005, Bonini, 2002, Ryoo et al., 2007, Bilen et al., 2006, Mallik and Lakhotia, 2009, Khurana et al., 2006, Mallik and Lakhotia, 2010, Jung et al., 2009, Carra et al., 2010, Park et al., 2011, Bohm et al., 2010, Fujikake et al., 2008) eye, with Scer\GAL4^GMR.PU (Vos et al., 2010, Chan et al., 2000, Bilen and Bonini, 2007) eye disc, with Scer\GAL4^GMR.PF (Fujikake et al., 2008) eye photoreceptor cell (Boeddrich et al., 2006) eye photoreceptor cell, with Scer\GAL4^GMR.PF (Bonini, 1999) eye photoreceptor cell, with Scer\GAL4^GMR.PU (Chan et al., 2000) Kenyon cell, with Scer\GAL4^30Y (Ghosh and Feany, 2004) Kenyon cell, with Scer\GAL4^elav-C155 (Khurana et al., 2010) LNv neuron, with Scer\GAL4^tim.PE (Kadener et al., 2006) neuron, with Scer\GAL4^179Y (Gunawardena et al., 2003) neuron, with Scer\GAL4^Appl.G1a (Gunawardena et al., 2003) ommatidium, with Scer\GAL4^GMR.PF (Kanuka et al., 2003, Park et al., 2011) ommatidium, with Scer\GAL4^GMR.PU (Arya et al., 2010) ommatidium \| conditional, with Scer\GAL4^ninaE.PT (Liu et al., 2012) ommatidium \| heat sensitive, with Scer\GAL4^GMR.PF, Scer\GAL80^ts.αTub84B (Tonoki et al., 2011) photoreceptor, with Scer\GAL4^elav-C155 (Li et al., 2008) photoreceptor \| conditional, with Scer\GAL4^ninaE.PD (Lessing and Bonini, 2008) photoreceptor \| conditional, with Scer\GAL4^ninaE.PT (Liu et al., 2012) pigment cell, with Scer\GAL4^GMR.PF (Warrick et al., 1998, Martin-Lanneree et al., 2006, Bonini, 1999) pigment granule, with Scer\GAL4^GMR.PF (Senoo-Matsuda et al., 2005) retina, with Scer\GAL4^GMR.PF (Warrick et al., 1998, Warrick et al., 1999, Senoo-Matsuda et al., 2005, Warrick et al., 2005, Bonini, 2002, Bilen et al., 2006, Mallik and Lakhotia, 2009, Ghosh and Feany, 2004, Lessing and Bonini, 2008) retina, with Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) retina \| progressive, with Scer\GAL4^GMR.PU (Chan et al., 2000) rhabdomere, with Scer\GAL4^elav-C155 (Warrick et al., 1998, Warrick et al., 1999) rhabdomere \| heat sensitive, with Scer\GAL4^GMR.PF, Scer\GAL80^ts.αTub84B (Tonoki et al., 2011) sensory neuron \| somatic clone, with Scer\GAL4^elav-C155 (Lessing and Bonini, 2008)
Other information

Allele	w^+mC
Reference(s)	(Warrick et al., 1998)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)	P{UAS-Hsap\MJD.tr-Q61} (Chan et al., 2000)
Comments

Stocks Listed in FlyBase ( 2 )
Bloomington	8141 w^; P{UAS-Hsap\MJD.tr-Q78}c37.3 8150 w^; P{UAS-Hsap\MJD.tr-Q78}c211.2
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Atx3Q78tr (Boeddrich et al., 2006) MJDtr(78Q) (Mizutani et al., 2005) MJDtr-Q78 (Senoo-Matsuda et al., 2005, Boeddrich et al., 2006, Tonoki et al., 2011, Fujikake et al., 2008) MJDtrQ78 (Bonini, 2002) MJDtr-Q78S (Fujikake et al., 2008) MJDtr-Q78W (Fujikake et al., 2008) P{UAS-Hsap\MJD.tr-Q78} (FlyBase, 1996-) P{UAS-MJDtr-Q78} (Umeda-Kameyama et al., 2007) SCA3-Q78 (Lee et al., 2004) SCA3tr-Q78 (Warrick et al., 2005, Jung and Bonini, 2007) SCA3trQ78 (Carra et al., 2010, Lessing and Bonini, 2008, Liu et al., 2012) SCA3trQ78_CAG (Li et al., 2008) UAS-MJD (Kadener et al., 2006) UAS-MJD-78Q (Gunawardena et al., 2003, Khurana et al., 2006) UAS-MJD78Q (Kadener et al., 2006) UAS-mjd78Q (Bohm et al., 2010) UASMJD-PQ78(s) (Bhutani et al., 2012) UAS-MJD-Q78 (Park et al., 2011) UAS-MJDtr78Q (Kadener et al., 2006) UAS-MJDtr-Q78 (Kanuka et al., 2003, Warrick et al., 1999, Bonini, 1999, Chan et al., 2000, Martin-Lanneree et al., 2006, Arya et al., 2010, Chang et al., 2011, Fujikake et al., 2008) uas-MJD-tr-Q78 (Ryoo et al., 2007) UAS-MJDtr-Q78(S) (Perez et al., 1998, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2010) UAS-MJDtr-Q78S (Fujikake et al., 2008) UAS-MJDtr-Q78W (Fujikake et al., 2008) UAS-Q78 (Moreno et al., 2002) UAS-SCA3 (Khurana et al., 2010) UAS-SCA3-78 (Ghosh and Feany, 2004) UAS-SCA3(78Q) (Carra et al., 2010) UAS-SCA3thQ78 (Vos et al., 2010) UAS-SCA3tr-Q78 (Bilen et al., 2006) UAS-SCA3trQ78 (Bilen and Bonini, 2007, Carra et al., 2010, Lessing and Bonini, 2008, Liu et al., 2012, Li et al., 2008) UAS-SCA3-trQ78 (Watson et al., 2008) UAS-SCA3trQ78(S) (Jung et al., 2009) UAS-SCA3trQ78_CAG (Li et al., 2008)
Secondary FlyBase IDs

References ( 41 )

Generate a list of	All references relating to this transgenic transposon ( 41 )

List References by type	Research paper ( 40 ) Review ( 1 )
Recent research papers ( 6 )
	Bhutani et al., 2012, Cell Death Dis. 3: e428 Dysregulation of core components of SCF complex in poly-glutamine disorders. [FBrf0219951] Butler et al., 2012, PLoS Genet. 8(2): e1002488 The Mitochondrial Chaperone Protein TRAP1 Mitigates α-Synuclein Toxicity. [FBrf0217423] Liu et al., 2012, Nature 482(7386): 519--523 The microRNA miR-34 modulates ageing and neurodegeneration in Drosophila. [FBrf0217556] Chang et al., 2011, PLoS Genet. 7(2): e1001288 Pathogenic VCP/TER94 Alleles Are Dominant Actives and Contribute to Neurodegeneration by Altering Cellular ATP Level in a Drosophila IBMPFD Model. [FBrf0213008] Park et al., 2011, Biochem. Biophys. Res. Commun. 404(2): 638--645 Normal prion protein in Drosophila enhances the toxicity of pathogenic polyglutamine proteins and alters susceptibility to oxidative and autophagy signaling modulators. [FBrf0212784] Tonoki et al., 2011, Genes Cells 16(5): 557--564 Aging causes distinct characteristics of polyglutamine amyloids in vivo. [FBrf0213535] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...