Recombinant construct P{rtTA.B}

General Information
Symbol	P{rtTA.B}	FlyBase ID	FBtp0009645
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	9 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Bieschke et al., 1998, Landis et al., 2003)
Uses	CV term Qualifiers & info Reference analytical tool Tn10\tetR \| Hsim\VP16 (Bieschke et al., 1998) binary system Tn10\tetR (Bieschke et al., 1998) binary system (regulatory) Act5C (Bieschke et al., 1998) ubiquitous expression Tn10\tetR \| Hsim\VP16 (Bieschke et al., 1998)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Bieschke et al., 1998) selectable marker (Bieschke et al., 1998)
Component Alleles
Allele	Tn10\tetR^{Act5C.T:Hsim\VP16}
Reference(s)	(Li and Tower, 2009, Stebbins and Yin, 2001, Bieschke et al., 1998, Landis et al., 2001, Zhang et al., 2010, Khokhar et al., 2008, Ford et al., 2007, Curtis et al., 2007, Hoe et al., 2011, Bhole et al., 2004)
Molecular data	Construct: The Act5C promoter drives expression of Tn10\tetR tagged with T:Hsim\VP16. (Ryu et al., 2001) Construct: The constitutive Act5C promoter and 5' untranslated region are fused to the coding region of the reverse tetracycline receptor, rtR, fused to the transcription activation domain of T:Hsim\VP16. The polyA sequences are from SV40. (Bieschke et al., 1998)
Phenotypic class	female semi-sterile, with MRG15^{dsRNA.Tn10\tetO} (Zhang et al., 2010) female semi-sterile, with MRG15^{MT1.Tn10\tetO.T:Ivir\HA1} (Zhang et al., 2010) heat stress response defective \| tetracycline conditional, with Hsp22^{Tn10\tetO.cBa} (Bhole et al., 2004) increased cell death \| P-stage, with Hsap\UBB^{Tn10\tetO.cHa} (Hoe et al., 2011) lethal, with ash1^PdL11A3 (Landis et al., 2001) lethal, with PdL2B1^PdL2B1 (Landis et al., 2001) lethal, with PdL3A^PdL3A (Landis et al., 2001) lethal, with PdL14B1^PdL14B1 (Landis et al., 2001) lethal, with PdL43C1^PdL43C1 (Landis et al., 2001) lethal, with PdL45C1^PdL45C1 (Landis et al., 2001) lethal, with PdL72A3^PdL72A3 (Landis et al., 2001) lethal, with PdL73A1^PdL73A1 (Landis et al., 2001) lethal, with PdL75A3^PdL75A3 (Landis et al., 2001) lethal \| P-stage, with Hsap\UBB^{Tn10\tetO.cHa} (Hoe et al., 2011) long lived, with Cct1^PdL8R128 (Landis et al., 2003) long lived, with cher^PdL8S64 (Landis et al., 2003) long lived, with fwd^PdL8S25 (Landis et al., 2003) long lived, with Rdh^PdL3E36 (Landis et al., 2003) long lived, with Sod2^{Tn10\tetO.cCa} (Curtis et al., 2007) long lived, with Sug^PdL2C33 (Landis et al., 2003) long lived, with VhaSFD^PdL4G14 (Landis et al., 2003) long lived, with α-Man-II^PdL19B3 (Landis et al., 2001) melanotic mass phenotype \| P-stage, with Hsap\UBB^{Tn10\tetO.cHa} (Hoe et al., 2011) oxidative stress response defective \| tetracycline conditional, with Hsp22^{Tn10\tetO.cBa} (Bhole et al., 2004) short lived, with ash1^PdL11A3 (Landis et al., 2001) short lived, with MRG15^{dsRNA.Tn10\tetO} (Zhang et al., 2010) short lived, with PdL2B1^PdL2B1 (Landis et al., 2001) short lived, with PdL14B1^PdL14B1 (Landis et al., 2001) short lived, with PdL35B1^PdL35B1 (Landis et al., 2001) short lived, with PdL43C1^PdL43C1 (Landis et al., 2001) short lived, with pum^PdL58A2 (Landis et al., 2001) short lived \| :heat sensitive \| tetracycline conditional, with Hsp22^{Tn10\tetO.cBa} (Bhole et al., 2004) short lived \| heat sensitive, with Sod2^{Tn10\tetO.cCa} (Curtis et al., 2007) short lived \| tetracycline conditional, with Sod2^{Tn10\tetO.cFa} (Ford et al., 2007) visible, with PdL35B1^PdL35B1 (Landis et al., 2001) visible, with pum^PdL3B2 (Landis et al., 2001) visible, with pum^PdL3G (Landis et al., 2001) visible, with pum^PdL58A2 (Landis et al., 2001) visible, with α-Man-II^PdL19B3 (Landis et al., 2001)
Phenotype manifest in	chorion \| drug conditional, with foxo^Y2-26 (Khokhar et al., 2008) chorion \| drug conditional, with ush^3-38 (Khokhar et al., 2008) dorsal appendage \| drug conditional, with dbr^3M1753 (Khokhar et al., 2008) dorsal appendage \| drug conditional, with edl^4M127 (Khokhar et al., 2008) dorsal appendage \| drug conditional, with foxo^Y2-26 (Khokhar et al., 2008) dorsal appendage \| drug conditional, with wmd^pter-2-4 (Khokhar et al., 2008) eye, with α-Man-II^PdL19B3 (Landis et al., 2001) micropyle \| drug conditional, with dbr^3M1753 (Khokhar et al., 2008) pupa, with Hsap\UBB^{Tn10\tetO.cHa} (Hoe et al., 2011) wing, with PdL35B1^PdL35B1 (Landis et al., 2001) wing, with pum^PdL3B2 (Landis et al., 2001) wing, with pum^PdL3G (Landis et al., 2001) wing, with pum^PdL58A2 (Landis et al., 2001)
Other information

Allele	w^+mC
Reference(s)	(Bieschke et al., 1998)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	construct_comment: Adaptation of rtTA (Tn10\tetR-T:Hsim\VP16 transactivator fusion protein) to Drosophila by use of Act5C promoter; used to test efficacy of a tetR-tetO binary system in Drosophila. (Bieschke et al., 1998)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Actin5c-tTA (Stebbins and Yin, 2001) P{rtTA.B} (FlyBase, 1996-, Landis et al., 2003, Ford et al., 2007) rtTA (Zhang et al., 2010, Khokhar et al., 2008, Li and Tower, 2009, Curtis et al., 2007, Hoe et al., 2011, Bhole et al., 2004)
Secondary FlyBase IDs

References ( 11 )
Research paper	Hoe et al., 2011, Aging 3(3): 237--261 Ubiquitin over-expression phenotypes and ubiquitin gene molecular misreading during aging in Drosophila melanogaster. [FBrf0213373] Zhang et al., 2010, Exp. Gerontol. 45(11): 825--833 Conditional inactivation of MRG15 gene function limits survival during larval and adult stages of Drosophila melanogaster. [FBrf0212068] Li and Tower, 2009, Mol. Genet. Genomics 281(2): 147--162 Adult-specific over-expression of the Drosophila genes magu and hebe increases life span and modulates late-age female fecundity. [FBrf0207375] Khokhar et al., 2008, Genetics 178(3): 1283--1293 Conditional switches for extracellular matrix patterning in Drosophila melanogaster. [FBrf0205261] Curtis et al., 2007, Genome Biol. 8(12): R262 Transcriptional profiling of MnSOD-mediated lifespan extension in Drosophila reveals a species-general network of aging and metabolic genes. [FBrf0205409] Ford et al., 2007, Exp. Gerontol. 42(6): 483--497 Alteration of Drosophila life span using conditional, tissue-specific expression of transgenes triggered by doxycyline or RU486/Mifepristone. [FBrf0200753] Bhole et al., 2004, Mech. Ageing Dev. 125(9): 651--663 Doxycycline-regulated over-expression of hsp22 has negative effects on stress resistance and life span in adult Drosophilamelanogaster. [FBrf0180481] Landis et al., 2003, Genome Biol. 4(2): R8 A search for doxycycline-dependent mutations that increase Drosophila melanogaster life span identifies the VhaSFD, Sugar baby, filamin, fwd and Cctl genes. [FBrf0157312] Landis et al., 2001, Genetics 158(3): 1167--1176 High-frequency generation of conditional mutations affecting Drosophila melanogaster development and life span. [FBrf0137252] Stebbins and Yin, 2001, Gene 270(1-2): 103--111 Adaptable doxycycline-regulated gene expression systems for Drosophila. [FBrf0136909] Bieschke et al., 1998, Mol. Gen. Genet. 258(6): 571--579 Doxycycline-induced transgene expression during Drosophila development and aging. [FBrf0103238]