Recombinant construct P{UAS-APP.695SPA4CT.T:Myc}

General Information
Symbol	P{UAS-APP.695SPA4CT.T:Myc}	FlyBase ID	FBtp0010536
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Fossgreen et al., 1998)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Fossgreen et al., 1998) selectable marker (Fossgreen et al., 1998)
Component Alleles
Allele	Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC}
Reference(s)	(Fossgreen et al., 1998, Gunawardena and Goldstein, 2001, Leyssen et al., 2005, Goguel et al., 2011)
Molecular data	Construct: A mutant form of Hsap\APP which lacks the extracellular domain (but contains the signal peptide, βA4 region and cytoplasmic tail) tagged at the C-terminus with T:Hsap\MYC is expressed under the control of Scer\UAS regulatory sequences. (Fossgreen et al., 1998)
Phenotypic class	increased cell death, with Scer\GAL4^Appl.G1a (Gunawardena and Goldstein, 2001) memory defective, with Scer\GAL4^{Mef2.247.Switch} (Goguel et al., 2011) neuroanatomy defective, with Scer\GAL4^P2.4.Pdf (Leyssen et al., 2005)
Phenotype manifest in	embryonic/larval nervous system, with Scer\GAL4^179Y (Gunawardena and Goldstein, 2001) embryonic/larval nervous system, with Scer\GAL4^Appl.G1a (Gunawardena and Goldstein, 2001) ventral adult lateral neuron & axon, with Scer\GAL4^P2.4.Pdf (Leyssen et al., 2005)
Other information

Allele	w^+mC
Reference(s)	(Fossgreen et al., 1998)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	6702 w^*; P{UAS-APP.695SPA4CT.T:Myc}XX
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-APP.695SPA4CT.T:Myc} (FlyBase, 1996-) P{UAS-Hsap\APP.695SPA4CT.T:Myc} (FlyBase, 1996-) SPA4CT (Leyssen et al., 2005) UAS-APP (Goguel et al., 2011) UAS-SPA4CT (Gunawardena and Goldstein, 2001)
Secondary FlyBase IDs

References ( 4 )
Research paper	Goguel et al., 2011, J. Neurosci. 31(3): 1032--1037 Drosophila amyloid precursor protein-like is required for long-term memory. [FBrf0212809] Leyssen et al., 2005, EMBO J. 24(16): 2944--2955 Amyloid precursor protein promotes post-developmental neurite arborization in the Drosophila brain. [FBrf0187512] Gunawardena and Goldstein, 2001, Neuron 32(3): 389--401 Disruption of axonal transport and neuronal viability by amyloid precursor protein mutations in Drosophila. [FBrf0141685] Fossgreen et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95(23): 13703--13708 Transgenic Drosophila expressing human amyloid precursor protein show gamma-secretase activity and a blistered-wing phenotype. [FBrf0105227]