Recombinant construct P{Mhc.CD8-GFP-Sh}

General Information
Symbol	P{Mhc.CD8-GFP-Sh}	FlyBase ID	FBtp0010818
Feature type	transgenic_transposon

Size		Expression data	GFP reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zito et al., 1999)
Uses	CV term Qualifiers & info Reference characterization Sh (Zito et al., 1999) reporter construct vital \| Sh (Zito et al., 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Zito et al., 1999) molecular tag (Zito et al., 1999) selectable marker (Zito et al., 1999)
Component Alleles
Allele	Sh^{Mhc.T:Avic\GFP-S65T,T:Hsap\CD8A}
Reference(s)	(Zito et al., 1999, Aberle et al., 2002, Parnas et al., 2001, Lee et al., 2009, McCabe et al., 2004, Pawlu et al., 2004, Laviolette et al., 2005, Meyer and Aberle, 2006, Beuchle et al., 2007, Siebert et al., 2009, Koch et al., 2008, Rasse et al., 2005, Banovic et al., 2010, Liu et al., 2010, Beck et al., 2012)
Molecular data	Construct: The Mhc promoter drives expression of a coding region fusion. The fusion contains; the extracellular and transmembrane domains of T:Hsap\CD8A; amino acids 479-503 of Sh B cytoplasmic domain and T:Avic\GFP^S65T. (Zito et al., 1999)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Zito et al., 1999)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for	larval somatic muscle (Aberle et al., 2002) larval neuromuscular junction (Aberle et al., 2002)
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Casper-MHC-CD8-GFP-Shaker (Lee et al., 2009) CD8-GFP-Sh (McCabe et al., 2004, Aberle et al., 2002, Parnas et al., 2001, Banovic et al., 2010, Meyer and Aberle, 2006, Beuchle et al., 2007, Siebert et al., 2009, Koch et al., 2008, Beck et al., 2012) CD8-Shaker^GFP (Rasse et al., 2005) MHC-CD8-GFP-Sh (Pawlu et al., 2004, Zito et al., 1999) MHC-CD8-GFP-Shaker (Liu et al., 2010) P{Mhc.CD8-GFP-Sh} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 15 )
Research paper	Beck et al., 2012, J. Neurosci. 32(20): 7058--7073 Regulation of Fasciclin II and Synaptic Terminal Development by the Splicing Factor Beag. [FBrf0218385] Banovic et al., 2010, Neuron 66(5): 724--738 Drosophila Neuroligin 1 Promotes Growth and Postsynaptic Differentiation at Glutamatergic Neuromuscular Junctions. [FBrf0211035] Liu et al., 2010, J. Neurosci. 30(35): 11624--11634 Distinct Presynaptic and Postsynaptic Dismantling Processes of Drosophila Neuromuscular Junctions during Metamorphosis. [FBrf0211694] Lee et al., 2009, Dev. Biol. 330(2): 250--262 Drosophila Atlastin regulates the stability of muscle microtubules and is required for synapse development. [FBrf0208045] Siebert et al., 2009, Genes Dev. 23(9): 1052--1062 Drosophila motor axons recognize and follow a Sidestep-labeled substrate pathway to reach their target fields. [FBrf0207941] Koch et al., 2008, Neuron 58(2): 210--222 Drosophila ankyrin 2 is required for synaptic stability. [FBrf0204749] Beuchle et al., 2007, Mech. Dev. 124(5): 390--406 Drosophila MICAL regulates myofilament organization and synaptic structure. [FBrf0200539] Meyer and Aberle, 2006, Development 133(20): 4035--4044 At the next stop sign turn right: the metalloprotease Tolloid-related 1 controls defasciculation of motor axons in Drosophila. [FBrf0193012] Laviolette et al., 2005, Genetics 170(2): 779--792 A genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth. [FBrf0187645] Rasse et al., 2005, Nat. Neurosci. 8(7): 898--905 Glutamate receptor dynamics organizing synapse formation in vivo. [FBrf0188163] McCabe et al., 2004, Neuron 41(6): 891--905 Highwire regulates presynaptic BMP signaling essential for synaptic growth. [FBrf0175022] Pawlu et al., 2004, Neuron 42(4): 607--618 Postfusional control of quantal current shape. [FBrf0179375] Aberle et al., 2002, Neuron 33(4): 545--558 wishful thinking Encodes a BMP Type II Receptor that Regulates Synaptic Growth in Drosophila. [FBrf0145177] Parnas et al., 2001, Neuron 32(3): 415--424 Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix. [FBrf0141687] Zito et al., 1999, Neuron 22(4): 719--729 Watching a synapse grow: noninvasive confocal imaging of synaptic growth in Drosophila. [FBrf0108312]