Recombinant construct P{UAS-robo-fra-Myc}

General Information
Symbol	P{UAS-robo-fra-Myc}	FlyBase ID	FBtp0011054
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Bashaw and Goodman, 1999)
Uses	CV term Qualifiers & info Reference characterization fra \| fusion construct (Bashaw and Goodman, 1999) fra \| robo (Bashaw and Goodman, 1999) robo \| fusion construct (Bashaw and Goodman, 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric protein (Bashaw and Goodman, 1999) molecular tag (Bashaw and Goodman, 1999) selectable marker (Bashaw and Goodman, 1999)
Component Alleles
Allele	fra::robo^{Scer\UAS.RF.T:Hsap\MYC}
Reference(s)	(Bhat, 2005, Bashaw et al., 2001, Bashaw and Goodman, 1999, Forsthoefel et al., 2005, Mauss et al., 2009)
Molecular data	Construct: This chimera has the extracellular, transmembrane and 67 amino acids of robo's cytoplasmic domain fused to the entire cytoplasmic domain of fra. Scer\UAS sequences drive expression and 6 T:Hsap\MYC tags mark its C terminus. (Bashaw and Goodman, 1999)
Phenotypic class	lethal \| partially, with Scer\GAL4^elav.PLu (Bashaw et al., 2001) neuroanatomy defective, with Scer\GAL4^Cha.7.4 (Mauss et al., 2009) neuroanatomy defective, with Scer\GAL4^elav.PLu (Bhat, 2005) neuroanatomy defective, with Scer\GAL4^elav-C155 (Forsthoefel et al., 2005)
Phenotype manifest in	axon, with Scer\GAL4^elav.PLu (Bashaw et al., 2001) cholinergic neuron, with Scer\GAL4^Cha.7.4 (Mauss et al., 2009) commissure, with Scer\GAL4^Cha.7.4 (Mauss et al., 2009) commissure, with Scer\GAL4^elav.PLu (Bashaw and Goodman, 1999) embryonic/larval somatic muscle \| precursor, with Scer\GAL4^how-24B (Bashaw and Goodman, 1999) longitudinal connective, with Scer\GAL4^elav.PLu (Bashaw and Goodman, 1999, Bhat, 2005) longitudinal connective, with Scer\GAL4^elav-C155 (Forsthoefel et al., 2005)
Other information

Allele	w^+mC
Reference(s)	(Bashaw and Goodman, 1999)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-robo-fra-Myc} (FlyBase, 1996-) Robo-Fra-Myc (Bashaw and Goodman, 1999) UAS-Robo^exFraⁱⁿ-myc (Mauss et al., 2009) UAS-Robo^extra-Fra^intra (Bhat, 2005) UASRobo-DCC (Bashaw et al., 2001) UAS-Robo-Fra-Myc (Forsthoefel et al., 2005)
Secondary FlyBase IDs

References ( 5 )
Research paper	Mauss et al., 2009, PLoS Biol. 7(9): e1000200 Midline signalling systems direct the formation of a neural map by dendritic targeting in the Drosophila motor system. [FBrf0208790] Bhat, 2005, Genetics 170(1): 149--159 Slit-roundabout signaling neutralizes Netrin-Frazzled-mediated attractant cue to specify the lateral positioning of longitudinal axon pathways. [FBrf0187630] Forsthoefel et al., 2005, Development 132(8): 1983--1994 The Abelson tyrosine kinase, the Trio GEF and Enabled interact with the Netrin receptor Frazzled in Drosophila. [FBrf0183931] Bashaw et al., 2001, J. Cell Biol. 155(7): 1117--1122 A novel Dbl family RhoGEF promotes Rho-dependent axon attraction to the central nervous system midline in Drosophila and overcomes Robo repulsion. [FBrf0141595] Bashaw and Goodman, 1999, Cell 97(7): 917--926 Chimeric axon guidance receptors: the cytoplasmic domains of slit and netrin receptors specify attraction versus repulsion. [FBrf0108593]