FB2025_01 , released February 20, 2025
Recombinant Construct: P{sal-lacZ.EME}
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General Information
Symbol
P{sal-lacZ.EME}
FlyBase ID
FBtp0011107
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZsal.EME
Also Known As
EME
Size
Expression Data
lacZ reporter
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZsal.EME
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Description

A genomic EcoRI fragment from within the regulatory regions of the spalt complex, within the first salr intron, is placed upstream of a weak P-element promoter followed by sequence encoding nuclear-localized lacZ.

(de Celis et al., 1999)

1.30kb fragment from a salr intron is fused upstream of lacZ.

(Barrio et al., 1999)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
late embryonic stage
embryonic epidermis | restricted
(Barrio et al., 1999)
presumptive embryonic/larval peripheral nervous system | restricted
(Barrio et al., 1999)
presumptive embryonic/larval central nervous system | restricted
(Barrio et al., 1999)
larval stage
haltere disc | restricted
(Barrio et al., 1999)
leg disc | restricted
(Barrio et al., 1999)
wing disc | restricted
(Barrio et al., 1999)
third instar larval stage
larval central nervous system | restricted
(Barrio et al., 1999)
Additional Information
Statement
Reference

Embryonic expression is detected in subsets of cells in the PNS where endogenous salm is expressed, as well as in cells in the CNS where endogenous salm and salr are not expressed. Expression is also detected in regions of the epidermis, partially overlapping with the endogenous expression of salm. In the larval CNS and in the wing disc, expression is detected in cells that normally express salm and salr, but additional ectopic expression is also detected.

(Barrio et al., 1999)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
EME
(Barrio et al., 1999, de Celis et al., 1999)
P{sal-lacZ.EME}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)