Embryonic expression is detected in subsets of cells in the PNS where endogenous salm is expressed, as well as in cells in the CNS where endogenous salm and salr are not expressed. Expression is also detected in regions of the epidermis, partially overlapping with the endogenous expression of salm. In the larval CNS and in the wing disc, expression is detected in cells that normally express salm and salr, but additional ectopic expression is also detected.