Recombinant construct M{M789^mini-white}

General Information
Symbol	M{M789^mini-white}	FlyBase ID	FBtp0011341
Feature type	transgenic_transposon

Size	5.8	Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon mariner element (Lohe et al., 1995)
Uses	CV term Qualifiers & info Reference carrier transposon Dmau\mariner (Lohe et al., 1995) characterization Dmau\mariner\T (Lohe et al., 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	5.8 (Lohe et al., 1995)
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Lohe et al., 1995)
Component Alleles
Allele	Dmau\mariner\T^pA2L
Reference(s)	(Lohe et al., 1995, Lozovsky et al., 2002, Lohe and Hartl, 1996)
Molecular data	Insertion of foreign DNA, including w^+mW.hs, in the SacI site at position 789 of the Mos1 transposable element. (Lohe and Hartl, 1996) Dmau\mariner\T coding region interrupted at position 789 by introduction of w^+mW.hs. (Lohe et al., 1995) Construct: Fragment of Mos1 up to nucleotide 787, including the Dmau\mariner 5' terminal repeat. (Lidholm et al., 1993)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Lohe et al., 1995, Lozovsky et al., 2002, Lohe and Hartl, 1996)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pM{M789^mini-white} (Lohe et al., 1995)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	M{M789^mini-white} (FlyBase, 1996-) SacW (Lozovsky et al., 2002)
Secondary FlyBase IDs

References ( 3 )
Research paper	Lozovsky et al., 2002, Genetics 160(2): 527--535 Unexpected Stability of mariner Transgenes in Drosophila. [FBrf0144896] Lohe and Hartl, 1996, Genetics 143(3): 1299--1306 Reduced germline mobility of a mariner vector containing exogenous DNA: effect of size or site? [FBrf0092616] Lohe et al., 1995, Genetics 140(1): 183--192 Genotypic effects, maternal effects and grand-maternal effects of immobilized derivatives of the transposable element mariner. [FBrf0082291]

Recombinant construct M{M789mini-white}

Recombinant construct M{M789^mini-white}