Recombinant construct P{UAS-EGFP}

General Information
Symbol	P{UAS-EGFP}	FlyBase ID	FBtp0011396
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	4 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Spana, 1999.9.27)
Uses	CV term Qualifiers & info Reference analytical tool cell marker (vital) (Spana, 1999.9.27) binary system (targeted) Avic\GFP (Spana, 1999.9.27) reporter construct Avic\GFP \| binary system (Spana, 1999.9.27)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Spana, 1999.9.27) reporter gene (Spana, 1999.9.27) selectable marker (Spana, 1999.9.27)
Component Alleles
Allele	Avic\GFP^E.Scer\UAS
Reference(s)	(Spana, 1999.9.27, Renault et al., 2003, Weihe et al., 2001, Liu et al., 2003, Stevaux et al., 2002, Wang et al., 2009, Gasque et al., 2005, Woodcock and Hughes, 2004, Kim et al., 2003, Ainsley et al., 2003, Liu et al., 2003, Tue et al., 2011, Layden et al., 2006, Stramer et al., 2005, Sage et al., 2008, Hyun et al., 2006, Hsu et al., 2004, Draper et al., 2007, Morris et al., 2006, Donlea et al., 2009, Rousset et al., 2010, Jana et al., 2011, Charroux and Royet, 2009, Skora and Spradling, 2010, Nishihara, 2010, Reim et al., 2012, Yang and O'Tousa, 2007, Ben-Shahar et al., 2010, Broderick et al., 2012)
Molecular data	Construct: Expression of a version of Avic\GFP that has two brightness/folding enhancing mutations and has been optimized for human codon usage is governed by Scer\UAS regulatory sequences. (Spana, 1999.9.27)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Spana, 1999.9.27)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAST} (Spana, 1999.9.27)
descendant(s)
Comments
	construct_comment: EGFP is a version of GFP from Clonetech that has two brightness/folding enhancing mutations and has been optimized for human codon usage.Constructed by Martin Zeidler. Progenitors are those of the plasmid, pP{UAS-EGFP}. (Spana, 1999.9.27) report comment: Avic\GFP^E.Scer\UAS is EGFP, a version of GFP from Clonetech that has two enhancing mutations. (Spana, 1999.9.27)
Stocks Listed in FlyBase ( 6 )
Bloomington	5428 P{UAS-EGFP}8, w¹¹¹⁸ 5431 w¹¹¹⁸; P{UAS-EGFP}5a.2 5430 w¹¹¹⁸; P{UAS-EGFP}34/TM3, Sb¹
Kyoto	108228 P{UAS-EGFP}8, w¹¹¹⁸ 108231 w¹¹¹⁸; P{UAS-EGFP}5a.2 108230 w¹¹¹⁸; P{UAS-EGFP}34/TM3, Sb¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-EGFP} (FlyBase, 1996-, Hsu et al., 2004, Yang and O'Tousa, 2007) P{UAS-GFP.E} (FlyBase, 1996-) P{UAS-GFP.EGFP} (FlyBase, 1996-) UAS-EGFP (Emery et al., 2000, Gasque et al., 2005, Woodcock and Hughes, 2004, Weihe et al., 2001, Hyun et al., 2006, Layden et al., 2006, Draper et al., 2007, Wang et al., 2009, Morris et al., 2006, Rousset et al., 2010, Charroux and Royet, 2009, Skora and Spradling, 2010, Nishihara, 2010, Ben-Shahar et al., 2010, Reim et al., 2012) UAS-eGFP (Ainsley et al., 2003, Liu et al., 2003, Kim et al., 2003, Liu et al., 2003, Stramer et al., 2005, Jana et al., 2011) UAS-egfp (Donlea et al., 2009) uas-EGFP (Tue et al., 2011) UAS-GFP (Broderick et al., 2012) UASp EGFP (Renault et al., 2003)
Secondary FlyBase IDs

References ( 30 )

Generate a list of	All references relating to this transgenic transposon ( 30 )

List References by type	Research paper ( 29 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 4 )
	Broderick et al., 2012, PLoS ONE 7(9): e44567 Drosophila ninjurin a induces nonapoptotic cell death. [FBrf0219544] Reim et al., 2012, Dev. Biol. 368(1): 28--43 The FGF8-related signals Pyramus and Thisbe promote pathfinding, substrate adhesion, and survival of migrating longitudinal gut muscle founder cells. [FBrf0218673] Jana et al., 2011, Mol. Biol. Cell 22(6): 769--781 Heterotrimeric kinesin-II is necessary and sufficient to promote different stepwise assembly of morphologically distinct bipartite cilia in Drosophila antenna. [FBrf0213262] Tue et al., 2011, Gene 473(1): 1--7 NF-Y transcriptionally regulates the Drosophila p53 gene. [FBrf0212943] Show all research papers ...