FlyBase Recombinant Construct Report: P{UAS-srcEGFP}

General Information

Symbol

P{UAS-srcEGFP}

FlyBase ID

FBtp0011397

Feature type

transgenic_transposable_element

Component Allele(s)

Avic\GFP^{EGFP.UAS.Tag:Myr(v-Src)}

Also Known As

UAS-srcEGFP, UAS-srcGFP, UAS-src-EGFP

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Avic\GFP^{EGFP.UAS.Tag:Myr(v-Src)}

Transgenic product class

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

EGFP

green fluorescent protein

Tagged with

Tag:Myr(v-Src)

membrane protein localization tag

Description

UAS regulatory sequences drive expression of EGFP fused to the Tag:Myr(v-Src) myristoylation signal.

(Spana, 1999.9.27)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^{EGFP.UAS.Tag:Myr(v-Src)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{UAST}

(Spana, 1999.9.27)

descendant(s)

Comments

report comment: Avic\GFP^{E.Scer\UAS.T:Uuuu\Myr4} is EGFP, a version of GFP from Clonetech that has two enhancing mutations, tagged with the 14-amino-acid myristylation domain of v-src.

(Spana, 1999.9.27)

construct_comment: EGFP is a version of GFP from Clonetech that has two brightness/folding enhancing mutations and has been optimized for human codon usage. srcEGFP constructs contain the 14-amino-acid myristylationdomain of v-src fused to EGFP.Constructed by Eric Spana. Progenitors are those of the plasmid, pP{UAS-srcEGFP}.

(Spana, 1999.9.27)

Stocks (4)

Stocks containing insertion(s) of this recombinant construct

Bloomington

5429

P{UAS-srcEGFP}M7A, y¹ w¹¹¹⁸

5432

y¹ w¹¹¹⁸; P{UAS-srcEGFP}M7E

Kyoto

108229

P{UAS-srcEGFP}M7A, w¹¹¹⁸

108232

w¹¹¹⁸; P{UAS-srcEGFP}M7E

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

P{UAS-GFP.E.src}

(FlyBase, 1996-)

P{UAS-GFP.src}

(FlyBase, 1996-)

P{UAS-srcEGFP}

(FlyBase, 1996-)

UAS srcGFP

(Guha and Kornberg, 2005)

UAS-EGFP

(Das et al., 2008)

UAS-Src-GFP

(Ninov et al., 2010)

UAS-SrcEGFP

(Ninov et al., 2010, Ninov et al., 2007)

UAS-SrcGFP

(Mathew et al., 2020)

UAS-src-EGFP

(Jiang et al., 2019, Krieser et al., 2007)

UAS-srcEGFP

(Stronach et al., 2014, Nonaka et al., 2013, Tung et al., 2013, Fujita et al., 2012, Siekhaus et al., 2010, Zeng et al., 2010, Kuraishi et al., 2009, Estrada et al., 2007, Gibson and Perrimon, 2005, Kirilly et al., 2005, Bruckner et al., 2004, Bruckner et al., 2004)

UAS-srcGFP

(Tamamouna et al., 2021, Ricolo and Araujo, 2020, Song et al., 2014, Yu et al., 2012)

UASmEGFP

(Schock and Perrimon, 2002)

UASsrcEGFP

(Schock and Perrimon, 2003)

srcEGFP

(Johansen et al., 2003)

Secondary FlyBase IDs

References (28)

Report Sections

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