Recombinant construct P{UAS-m4.A}

General Information
Symbol	P{UAS-m4.A}	FlyBase ID	FBtp0011498
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Apidianakis et al., 1999)
Uses	CV term Qualifiers & info Reference binary system (targeted) m4 (Apidianakis et al., 1999) characterization m4 (Apidianakis et al., 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Apidianakis et al., 1999) selectable marker (Apidianakis et al., 1999)
Component Alleles
Allele	E(spl)m4-BFM^Scer\UAS.cAa
Reference(s)	(Apidianakis et al., 1999, Tapanes-Castillo and Baylies, 2004, Kumar and Moses, 2001, Nagel et al., 2000, Kumar and Moses, 2001, San-Juán and Baonza, 2011, Parks and Muskavitch, 2009.2.4)
Molecular data	Construct: The complete m4 cDNA is under the control of Scer\UAS sequences. (Apidianakis et al., 1999)
Phenotypic class	visible, with Scer\GAL4^455.2 (Apidianakis et al., 1999) visible, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) visible, with Scer\GAL4^pnr-MD237 (Apidianakis et al., 1999) visible, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) visible, with Scer\GAL4^sca-T3 (Apidianakis et al., 1999) visible \| somatic clone, with Scer\GAL4^Ubx.PdC (Apidianakis et al., 1999)
Phenotype manifest in	dorsal row & sensory mother cell, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^455.2 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^pnr-MD237 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^sca-T3 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^455.2 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^pnr-MD237 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^sca-T3 (Apidianakis et al., 1999) microchaeta \| somatic clone, with Scer\GAL4^Ubx.PdC (Apidianakis et al., 1999) scutellar bristle, with Scer\GAL4^455.2 (Apidianakis et al., 1999) scutellar bristle, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) scutellar bristle \| ectopic, with Scer\GAL4^455.2 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^455.2 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^pnr-MD237 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^sca-T3 (Apidianakis et al., 1999) sensillum campaniformium of anterior crossvein, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) sensillum campaniformium of anterior crossvein \| precursor, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) sensillum campaniformium of dorsal radius, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) sensory mother cell \| ectopic, with Scer\GAL4^bi-omb-Gal4 (Nagel et al., 2000)
Other information

Allele	w^+mC
Reference(s)	(Apidianakis et al., 1999)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 2 )
Bloomington	26679 w^; P{UAS-m4.A}15.5 26678 w^; P{UAS-m4.A}15.21
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-m4.A} (FlyBase, 1996-, Parks and Muskavitch, 2009.2.4) UAS-E(spl) m4 (San-Juán and Baonza, 2011) UAS-E(spl)m4 (San-Juán and Baonza, 2011) UAS-m4 (Tapanes-Castillo and Baylies, 2004, Kumar and Moses, 2001, Kumar and Moses, 2001, Nagel et al., 2000, Apidianakis et al., 1999)
Secondary FlyBase IDs

References ( 7 )
Research paper	San-Juán and Baonza, 2011, Dev. Biol. 352(1): 70--82 The bHLH factor deadpan is a direct target of Notch signaling and regulates neuroblast self-renewal in Drosophila. [FBrf0213150] Tapanes-Castillo and Baylies, 2004, Development 131(10): 2359--2372 Notch signaling patterns Drosophila mesodermal segments by regulating the bHLH transcription factor twist. [FBrf0174582] Kumar and Moses, 2001, Development 128(14): 2689--2697 The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development. [FBrf0138358] Kumar and Moses, 2001, Cell 104(5): 687--697 EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. [FBrf0134490] Nagel et al., 2000, Mech. Dev. 98(1-2): 19--28 Neural hyperplasia induced by RNA interference with m4/m gene activity. [FBrf0133204] Apidianakis et al., 1999, Mech. Dev. 86(1,2): 39--50 Overexpression of the m4 and malpha genes of the E(spl)-complex antagonizes notch mediated lateral inhibition. [FBrf0111325]
Personal communication to FlyBase	Parks and Muskavitch, 2009.2.4, P{UAS-m4.A} insertions. P{UAS-m4.A} insertions. [FBrf0206651]