Recombinant construct P{UAS-Rho1.Sph}

General Information
Symbol	P{UAS-Rho1.Sph}	FlyBase ID	FBtp0011549
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Prokopenko et al., 1999)
Uses	CV term Qualifiers & info Reference binary system (targeted) Rho1 (Prokopenko et al., 1999) characterization Rho1 (Prokopenko et al., 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Prokopenko et al., 1999)
Component Alleles
Allele	Rho1^Scer\UAS.cMa
Reference(s)	(Prokopenko et al., 1999, Hu et al., 2005, Billuart et al., 2001, Speck et al., 2003, Kunwar et al., 2003, Warner and Longmore, 2009, Chen et al., 2004, Pazman et al., 2000, Lee et al., 2000, Ng and Luo, 2004, Stramer et al., 2005, Vidal et al., 2006, Chung et al., 2007, Neisch et al., 2010, van Impel et al., 2009, Renault et al., 2010)
Molecular data	Construct: Scer\UAS sequences drive wild-type Rho1. (Prokopenko et al., 1999)
Phenotypic class	increased cell death, with Scer\GAL4^dpp.blk1 (Neisch et al., 2010) increased cell death, with Scer\GAL4^ptc-559.1 (Vidal et al., 2006) lethal, with Scer\GAL4^dpp.blk1 (Neisch et al., 2010) planar polarity defective, with Scer\GAL4^hs.2sev (Pazman et al., 2000) visible, with Scer\GAL4^dpp.blk1 (Neisch et al., 2010)
Phenotype manifest in	disc epithelium proper & dorsal mesothoracic disc, with Scer\GAL4^ptc-559.1 (Vidal et al., 2006) ommatidium, with Scer\GAL4^GMR.PF (Hu et al., 2005) ommatidium, with Scer\GAL4^hs.2sev (Pazman et al., 2000) photoreceptor cell, with Scer\GAL4^GMR.PF (Hu et al., 2005) wing blade, with Scer\GAL4^dpp.blk1 (Neisch et al., 2010) wing disc, with Scer\GAL4^Bx-MS1096 (Speck et al., 2003) wing disc, with Scer\GAL4^ptc-559.1 (Speck et al., 2003) wing disc \| third instar larval stage, with Scer\GAL4^dpp.blk1 (Neisch et al., 2010)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	7334 w^*; P{UAS-Rho1.Sph}2.1
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-Rho1.Sph} (FlyBase, 1996-) rho1 (Vidal et al., 2006) UAS::Rho1.Sph (van Impel et al., 2009) UAS Rho (Renault et al., 2010) UAS-Rho1 (Ng and Luo, 2004, Chen et al., 2004, Speck et al., 2003, Warner and Longmore, 2009) UASrho1 (Renault et al., 2010) UAS-Rho1⁺ (Neisch et al., 2010) UAS-Rho1-Sph (Prokopenko et al., 1999) UAS-RhoA (Billuart et al., 2001, Pazman et al., 2000, Chung et al., 2007) UAS-RhoA1^wt (Kunwar et al., 2003) UAS-RhoA(wt) (Lee et al., 2000) UAS-Rhowt (Stramer et al., 2005)
Secondary FlyBase IDs

References ( 16 )
Research paper	Neisch et al., 2010, J. Cell Biol. 189(2): 311--323 Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. [FBrf0210554] Renault et al., 2010, Development 137(11): 1815--1823 Lipid phosphate phosphatase activity regulates dispersal and bilateral sorting of embryonic germ cells in Drosophila. [FBrf0210764] van Impel et al., 2009, Development 136(5): 813--822 Regulation of the Rac GTPase pathway by the multifunctional Rho GEF Pebble is essential for mesoderm migration in the Drosophila gastrula. [FBrf0206776] Warner and Longmore, 2009, J. Cell Biol. 185(6): 1111--1125 Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia. [FBrf0208183] Chung et al., 2007, Genetics 176(2): 891--903 The balance between the novel protein target of wingless and the Drosophila Rho-associated kinase pathway regulates planar cell polarity in the Drosophila wing. [FBrf0202398] Vidal et al., 2006, Dev. Cell 10(1): 33--44 Csk-deficient boundary cells are eliminated from normal Drosophila epithelia by exclusion, migration, and apoptosis. [FBrf0190225] Hu et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(12): 4613--4618 Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. [FBrf0191476] Stramer et al., 2005, J. Cell Biol. 168(4): 567--573 Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration. [FBrf0184064] Chen et al., 2004, Curr. Biol. 14(4): 309--313 Rho-LIM kinase signaling regulates ecdysone-induced gene expression and morphogenesis during Drosophila metamorphosis. [FBrf0174477] Ng and Luo, 2004, Neuron 44(5): 779--793 Rho GTPases Regulate Axon Growth through Convergent and Divergent Signaling Pathways. [FBrf0180582] Kunwar et al., 2003, PLoS Biol. 1(3): e80 Tre1, a G protein-coupled receptor, directs transepithelial migration of Drosophila germ cells. [FBrf0167988] Speck et al., 2003, Nature 421(6918): 83--87 Moesin functions antagonistically to the Rho pathway to maintain epithelial integrity. [FBrf0155521] Billuart et al., 2001, Cell 107(2): 195--207 Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610] Lee et al., 2000, Neuron 25(2): 307--316 Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis. [FBrf0127188] Pazman et al., 2000, Development 127(8): 1715--1725 Rasputin, the Drosophila homologue of the RasGAP SH3 binding protein, functions in ras- and -mediated signaling. [FBrf0127269] Prokopenko et al., 1999, Genes Dev. 13(17): 2301--2314 A putative exchange factor for rho1 GTPase is required for initiation of cytokinesis in Drosophila. [FBrf0111464]