Recombinant construct P{UAS-disp.HA1}

General Information
Symbol	P{UAS-disp.HA1}	FlyBase ID	FBtp0011804
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Burke et al., 1999)
Uses	CV term Qualifiers & info Reference binary system (targeted) disp (Burke et al., 1999) characterization disp (Burke et al., 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Burke et al., 1999) binary component (Burke et al., 1999) molecular tag (Burke et al., 1999) selectable marker (Burke et al., 1999)
Component Alleles
Allele	disp^{Scer\UAS.T:Ivir\HA1}
Reference(s)	(Burke et al., 1999, Gallet et al., 2006, Deshpande and Schedl, 2005, Casali, 2010, Callejo et al., 2011)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of disp tagged at the 3' end with a triple T:Ivir\HA1 tag. αTub84B 3' UTR sequences are fused downstream of the T:Ivir\HA1 sequences. (Burke et al., 1999)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Burke et al., 1999)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-disp.HA1} (FlyBase, 1996-) UAS-disp (Burke et al., 1999, Deshpande and Schedl, 2005) UAS Disp (Casali, 2010) UAS-Disp (Callejo et al., 2011) UAS-disp^HA (Gallet et al., 2006)
Secondary FlyBase IDs

References ( 5 )
Research paper	Callejo et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(31): 12591--12598 Feature Article: From the Cover: Dispatched mediates Hedgehog basolateral release to form the long-range morphogenetic gradient in the Drosophila wing disk epithelium. [FBrf0214615] Casali, 2010, Sci. Signal. 3(136): ra63 Self-induced patched receptor down-regulation modulates cell sensitivity to the hedgehog morphogen gradient. [FBrf0214124] Gallet et al., 2006, Development 133(3): 407--418 Cholesterol modification is necessary for controlled planar long-range activity of Hedgehog in Drosophila epithelia. [FBrf0190290] Deshpande and Schedl, 2005, Dev. Cell 9(5): 629--638 HMGCoA reductase potentiates hedgehog signaling in Drosophila melanogaster. [FBrf0190201] Burke et al., 1999, Cell 99(7): 803--815 Dispatched, a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells. [FBrf0122981]