FB2021_02, released April 13, 2021

Recombinant Construct: P{HsapRAF1.Δ305.FRT}

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General Information
Symbol
P{Hsap\RAF1.Δ305.FRT}
FlyBase ID
FBtp0012245
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\RAF1Δ305.FRT.αTub84B.Tag:Myr(Src64B)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(Greenwood and Struhl, 1999)
Construct components
Product class / Tool use(s)
Marker(s)
f+t13
y+t7.7
Component allele Hsap\RAF1Δ305.FRT.αTub84B.Tag:Myr(Src64B)
Product class / Tool use(s)
Regulatory region(s)
αTub84B
Tagged with
Tag:Myr(Src64B)
Also carries
FRT
Description

An activated form of Hsap\RAF1 (which contains a 305 amino acid N-terminal truncation) and tagged with a myristoylation sequence (Tag:Myr(Src64B)) is separated from an αTub84B promoter by an FRT cassette containing f+t13 and y+t7.7.

(Greenwood and Struhl, 1999)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
molecular
tag
(Greenwood and Struhl, 1999)
selectable
marker
(Greenwood and Struhl, 1999)
selectable
marker
(Greenwood and Struhl, 1999)
Component Alleles
Allele
Hsap\RAF1Δ305.FRT.αTub84B.Tag:Myr(Src64B)
Reference(s)
(Greenwood and Struhl, 1999)
Molecular data

An activated form of Hsap\RAF1 (which contains a 305 amino acid N-terminal truncation) and tagged with a myristoylation sequence (Tag:Myr(Src64B)) is separated from an αTub84B promoter by an FRT cassette containing f+t13 and y+t7.7.

(Greenwood and Struhl, 1999)
Phenotype Data
Phenotypic ClassPhenotype Manifest InDetailed Description
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{Hsap\RAF1.Δ305.FRT}
(FlyBase, 1996-)
Tubulinα1>f+y+>raf*
(Greenwood and Struhl, 1999)
Secondary FlyBase IDs
    References (2)