Recombinant construct P{UAS-ΔNdronc.C->A}

General Information
Symbol	P{UAS-ΔNdronc.C->A}	FlyBase ID	FBtp0012459
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Meier et al., 2000)
Uses	CV term Qualifiers & info Reference binary system (targeted) Nc (Meier et al., 2000) characterization Nc (Meier et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Meier et al., 2000) selectable marker (Meier et al., 2000)
Component Alleles
Allele	Nc^{ΔN.C-A.Scer\UAS}
Reference(s)	(Meier et al., 2000, Geisbrecht and Montell, 2004, Meier, 2000.4.5, Bauer et al., 2005, Williams et al., 2006)
Molecular data	Construct: A catalytically inactive and truncated coding sequence of Nc is expressed under the control of Scer\UAS sequences. Includes nucleotides 706 to 1721, missing its prodomain and with its catalytic Cys mutated to Ala. (Meier, 2000.4.5)
Phenotypic class	neuroanatomy defective, with Scer\GAL4^ppk.1.9 (Williams et al., 2006)
Phenotype manifest in	dendrite, with Scer\GAL4^ppk.1.9 (Williams et al., 2006) dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9 (Williams et al., 2006)
Other information

Allele	w^+mC
Reference(s)	(Meier et al., 2000)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	DRONC^C>A (Williams et al., 2006) DRONC-DN (Bauer et al., 2005) P{UAS-ΔNdronc.C->A} (FlyBase, 1996-) pUAST-(Delta)N dronc C->A (Meier, 2000.4.5) UAS-Dronc-ΔC->A (Geisbrecht and Montell, 2004) ΔN dronc C->A (Meier et al., 2000)
Secondary FlyBase IDs

References ( 5 )
Research paper	Williams et al., 2006, Nat. Neurosci. 9(10): 1234--1236 Local caspase activity directs engulfment of dendrites during pruning. [FBrf0192062] Bauer et al., 2005, Curr. Biol. 15(22): 2063--2068 Neuronal expression of p53 dominant-negative proteins in adult Drosophila melanogaster extends life span. [FBrf0189997] Geisbrecht and Montell, 2004, Cell 118(1): 111--125 A role for Drosophila IAP1-mediated caspase inhibition in Rac-dependent cell migration. [FBrf0179220] Meier et al., 2000, EMBO J. 19(4): 598--611 The Drosophila caspase DRONC is regulated by DIAP1. [FBrf0125395]
Personal communication to FlyBase	Meier, 2000.4.5, DRONC constructs. DRONC constructs. [FBrf0126737]