Recombinant construct P{GUS-p53.259H}

General Information
Symbol	P{GUS-p53.259H}	FlyBase ID	FBtp0012566
Feature type	transgenic_transposon	Created / Updated	2000-06-01/2000-06-01

Size		Expression data
Location-dependent role		Associated insertions	1 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Brodsky et al., 2000)
Uses	CV term Qualifiers & info Reference characterization p53 (Brodsky et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
p53^259H.GUS
Reference(s)	(Brodsky et al., 2000, Sidorov et al., 2002, Sidorov and Belitskii, 2002, Jassim et al., 2003, Shcherbata et al., 2004, Bauer et al., 2005, Bauer et al., 2005, Singh et al., 2006)
Molecular data	Construct: Full length coding region for p53 is expressed from both GMR and Scer\UAS regulatory sequences. Amino acid replacement: R259H. (Brodsky et al., 2000)
Phenotypic class	cell death increase \| conditional qualifier (Jassim et al., 2003) cell death decrease, with Scer\GAL4^en-e16E (Brodsky et al., 2000) long lived \| conditional RU486, with Scer\GAL4^{elav.Switch.PO} (Bauer et al., 2005) chemical resistant \| conditional RU486, with Scer\GAL4^{elav.Switch.PO} (Bauer et al., 2005)
Phenotype manifest in	eye \| conditional qualifier (Jassim et al., 2003) dorsal mesothoracic disc \| posterior compartment, with Scer\GAL4^en-e16E (Brodsky et al., 2000)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Dmp53(259H) (Brodsky et al., 2000) DN-Dmp53-259H (Bauer et al., 2005) GUS-Dmp53²⁵⁹ (Jassim et al., 2003) P(GUS.p53.259H) (Sidorov and Belitskii, 2002) P{GUS*p53.259H} (Sidorov et al., 2002) P{GUS-p53.259H} (FlyBase, 1996-) P{w[+mC]= GUS-p53.259H} (Shcherbata et al., 2004) UAS-p35^DN (Singh et al., 2006)
Secondary FlyBase IDs

References ( 9 )
Research paper	Singh et al., 2006, Development 133(23): 4771--4781 Lobe and Serrate are required for cell survival during early eye development in Drosophila. [FBrf0192707] Bauer et al., 2005, Curr. Biol. 15(22): 2063--2068 Neuronal expression of p53 dominant-negative proteins in adult Drosophila melanogaster extends life span. [FBrf0189997] Shcherbata et al., 2004, Development 131(13): 3169--3181 The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notch-dependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions. [FBrf0179442] Jassim et al., 2003, EMBO J. 22(20): 5622--5632 Dmp53 protects the Drosophila retina during a developmentally regulated DNA damage response. [FBrf0173083] Sidorov and Belitskii, 2002, Genetika, Moscow 38(7): 929--936 [The effect of transposon P(GUS.p53.259H)on the frequency of tumor clones in Drosophila melanogaster wtsp2/+ heterozygotes.] [FBrf0152051] Brodsky et al., 2000, Cell 101(1): 103--113 Drosophila p53 binds a damage response element at the reaper locus. [FBrf0127026]
Supplementary material	Bauer et al., 2005, Curr. Biol. 15(22): [FBrf0198673]
Abstract	Sidorov et al., 2002, A. Dros. Res. Conf. 43: 987C P{GUS&?p53.259H} raises carcinogen-induced wts tumor frequencies in wts/+ heterozygotes Drosophila melanogaster. [FBrf0145693]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]