Recombinant construct P{ato-GAL4.3.6}

General Information
Symbol	P{ato-GAL4.3.6}	FlyBase ID	FBtp0012626
Feature type	transgenic_transposon	Created / Updated	2000-06-01/2000-06-01

Size		Expression data	GAL4 reporter/driver
Location-dependent role		Associated insertions	3 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Hassan et al., 2000)
Uses	CV term Qualifiers & info Reference binary system (regulatory) ato (Hassan et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Hassan et al., 2000)
Component Alleles
Scer\GAL4^ato.3.6
Reference(s)	(Hassan et al., 2000, Brodu et al., 2002, Zheng et al., 2006, Inbal et al., 2003, Sen et al., 2004, Wang et al., 2002, Morales et al., 2002, Jhaveri et al., 2004, Inbal et al., 2004, Kraut and Zinn, 2004, Wang et al., 2006, Srahna et al., 2006, Grillenzoni et al., 2007, Garg et al., 2007)
Molecular data	Construct: A 3.6kb genomic fragment from directly upstream of the ato open reading frame is fused upstream of a minimal promoter driving Scer\GAL4 expression. (Hassan et al., 2000)
Phenotypic class	neuroanatomy defective, with Fmr1^Scer\UAS.cZa (Morales et al., 2002) developmental rate defective, with rpr^Scer\UAS.cJa (Hassan et al., 2000) lethal, with rpr^Scer\UAS.cJa (Hassan et al., 2000) aging defective, with Rcom\RA^Scer\UAS.cBa (Hassan et al., 2000) lethal, with Rcom\RA^Scer\UAS.cBa (Hassan et al., 2000) neuroanatomy defective, with Fmr1^{I307N.Scer\UAS} (Morales et al., 2002) neuroanatomy defective, with lea^EP2582 (Kraut and Zinn, 2004) neuroanatomy defective, with btl^DN.Scer\UAS (Srahna et al., 2006) neuroanatomy defective, with btl^Scer\UAS.cDa (Srahna et al., 2006) neuroanatomy defective, with Rac1^N17.Scer\UAS (Srahna et al., 2006) neuroanatomy defective, with Rac1^V12.Scer\UAS (Srahna et al., 2006) neuroanatomy defective, with Rac1^Scer\UAS.cLa (Srahna et al., 2006) neuroanatomy defective, with hep^CA.Scer\UAS (Srahna et al., 2006) neuroanatomy defective, with bsk^DN.Scer\UAS (Srahna et al., 2006)
Phenotype manifest in	larval oenocyte \| ectopic, with rho^{Scer\UAS.cdCa} (Brodu et al., 2002) embryonic mesothoracic segment, with rho^{Scer\UAS.cdCa} (Brodu et al., 2002) embryonic metathoracic segment, with rho^{Scer\UAS.cdCa} (Brodu et al., 2002) embryonic prothoracic segment, with rho^{Scer\UAS.cdCa} (Brodu et al., 2002) dorsal cluster neuron, with Fmr1^Scer\UAS.cZa (Morales et al., 2002) chordotonal organ \| ectopic, with Mmus\Atoh1^Scer\UAS.cBa (Wang et al., 2002) medulla, with Mmus\Atoh1^Scer\UAS.cBa (Wang et al., 2002) abdominal lateral pentascolopidial chordotonal organ lch5 & stage 16 embryo, with en::hth^{1-430.Scer\UAS} (Inbal et al., 2004) lch5 ligament attachment cell & stage 16 embryo, with en::hth^{1-430.Scer\UAS} (Inbal et al., 2004) lobula, with ato^Scer\UAS.cJa (Hassan et al., 2000) medulla, with ato^Scer\UAS.cJa (Hassan et al., 2000) optic chiasma, with ato^Scer\UAS.cJa (Hassan et al., 2000) larval oenocyte \| ectopic, with abd-A^{1dB5.Scer\UAS} (Brodu et al., 2002) embryonic mesothoracic segment, with abd-A^{1dB5.Scer\UAS} (Brodu et al., 2002) embryonic metathoracic segment, with abd-A^{1dB5.Scer\UAS} (Brodu et al., 2002) embryonic prothoracic segment, with abd-A^{1dB5.Scer\UAS} (Brodu et al., 2002) dorsal cluster neuron, with Fmr1^{I307N.Scer\UAS} (Morales et al., 2002) dorsal cluster neuron & neurite, with Fmr1^{I307N.Scer\UAS} (Morales et al., 2002) abdominal lateral pentascolopidial chordotonal organ lch5, with lea^EP2582 (Kraut and Zinn, 2004) lobula, with N^{intra.GS.Scer\UAS} (Hassan et al., 2000) medulla, with N^{intra.GS.Scer\UAS} (Hassan et al., 2000) larval oenocyte \| ectopic, with spi^s.Scer\UAS (Brodu et al., 2002) embryonic mesothoracic segment, with spi^s.Scer\UAS (Brodu et al., 2002) embryonic metathoracic segment, with spi^s.Scer\UAS (Brodu et al., 2002) embryonic prothoracic segment, with spi^s.Scer\UAS (Brodu et al., 2002) axon & dorsal cluster neuron & adult brain, with btl^DN.Scer\UAS (Srahna et al., 2006) axon & dorsal cluster neuron, with btl^Scer\UAS.cDa (Srahna et al., 2006) axon & dorsal cluster neuron, with Rac1^N17.Scer\UAS (Srahna et al., 2006) axon & dorsal cluster neuron, with Rac1^V12.Scer\UAS (Srahna et al., 2006) axon & dorsal cluster neuron, with Rac1^Scer\UAS.cLa (Srahna et al., 2006) axon & dorsal cluster neuron, with hep^CA.Scer\UAS (Srahna et al., 2006) axon & dorsal cluster neuron, with bsk^DN.Scer\UAS (Srahna et al., 2006)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference pupal stage \| 36 hr sensillum coeloconicum (Sen et al., 2004) pupal stage \| 22-30 hr antennal disc \| restricted (Sen et al., 2004) larval stage \| third instar larval optic lobe (Hassan et al., 2000) pupal stage \| 22-30 hr external sensory organ precursor cell <of> antennal disc (Sen et al., 2004) larval stage \| third instar larval brain \| restricted (Hassan et al., 2000) pupal stage \| >= 30hr antennal lobe \| restricted (Jhaveri et al., 2004) pupal stage \| 22-30 hr external sensory organ precursor cell \| subset (Sen et al., 2004) embryonic stage \| stage 13 embryonic central brain \| restricted (Hassan et al., 2000) adult stage adult brain \| restricted (Hassan et al., 2000) adult stage optic neuropil (Hassan et al., 2000) embryonic stage \| stage 11 external sensory organ precursor cell (Hassan et al., 2000)
Additional Information
Statement Reference In embryonic brain, initially expressed in the sensory organ precursors of the embryonic CHOs. At stage 13, 3-5 cells in each brain hemisphere initiate expression; this expression transient and is no longer detectable in stage 15 embryos. In larval brain, expression is reinitiated at L3 in a cluster of 25-30 neurons in each brain lobe. Each cluster of neurons sends a bundle of axons ventrally and then contralaterally toward the opposite optic lobes; at the junction between the central brain and the developing lobula, the axons derived from the contralateral cluster branch out of the commissure, into the developing optic lobes. In adult brain, expressed in a dorsal cluster and in two ventral clusters of neurons, which extend into elaborate axonal innervation patterns in the optic lobes, lobula, and medulla. (Hassan et al., 2000) Expression is observed in the proneural region of the antennal disc, and subsequently in all cells of the coelonconic sense organs. (Sen et al., 2004)

Assay mode	distribution deduced from reporter (Gal4 UAS) (Sen et al., 2004) distribution deduced from reporter (Gal4 UAS) (Sen et al., 2004) distribution deduced from reporter (Gal4 UAS) (Hassan et al., 2000) distribution deduced from reporter (Gal4 UAS) (Sen et al., 2004) distribution deduced from reporter (Gal4 UAS) (Hassan et al., 2000) distribution deduced from reporter (Gal4 UAS) (Jhaveri et al., 2004) distribution deduced from reporter (Gal4 UAS) (Sen et al., 2004) distribution deduced from reporter (Gal4 UAS) (Hassan et al., 2000) distribution deduced from reporter (Gal4 UAS) (Hassan et al., 2000) distribution deduced from reporter (Gal4 UAS) (Hassan et al., 2000) distribution deduced from reporter (Gal4 UAS) (Hassan et al., 2000)
Marker for	external sensory organ precursor cell <of> antennal disc (Sen et al., 2004) sensillum coeloconicum (Sen et al., 2004)
Reflects expression of	ato (Hassan et al., 2000, Sen et al., 2004, Jhaveri et al., 2004)
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	ato-Gal4 (Inbal et al., 2004, Jhaveri et al., 2004, Inbal et al., 2003, Grillenzoni et al., 2007) ato-GAL4 (Sen et al., 2004, Brodu et al., 2002, Wang et al., 2006) atoGal4 (Wang et al., 2002, Srahna et al., 2006, Garg et al., 2007) atoGal4-14A (Morales et al., 2002) Atonal-GAL4 (Kraut and Zinn, 2004) P{ato-GAL4.3.6} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 15 )
Research paper	Garg et al., 2007, Genetics 175(2): 659--669 Antagonizing scalloped with a novel vestigial construct reveals an important role for scalloped in Drosophila melanogaster leg, eye and optic lobe development. [FBrf0193374] Grillenzoni et al., 2007, Dev. Genes Evol. 217(3): 209--219 Role of proneural genes in the formation of the larval olfactory organ of Drosophila. [FBrf0193556] Srahna et al., 2006, PLoS Biol. 4(11): A signaling network for patterning of neuronal connectivity in the Drosophila brain. [FBrf0192599] Wang et al., 2006, Development 133(7): 1231--1240 Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain. [FBrf0190330] Zheng et al., 2006, EMBO J. 25(3): 615--627 Baboon/dSmad2 TGF-beta signaling is required during late larval stage for development of adult-specific neurons. [FBrf0190369] Inbal et al., 2004, Dev. Cell 7(2): 241--250 Recruitment of ectodermal attachment cells via an EGFR-dependent mechanism during the organogenesis of Drosophila proprioceptors. [FBrf0180120] Jhaveri et al., 2004, Development 131(9): 1903--1912 Positioning sensory terminals in the olfactory lobe of Drosophila by Robo signaling. [FBrf0174568] Kraut and Zinn, 2004, Curr. Biol. 14(15): 1319--1329 Roundabout 2 regulates migration of sensory neurons by signaling in trans. [FBrf0180051] Sen et al., 2004, Mech. Dev. 121(1): 65--78 Programmed cell death and context dependent activation of the EGF pathway regulate gliogenesis in the Drosophila olfactory system. [FBrf0167846] Inbal et al., 2003, Development 130(11): 2467--2478 Multiple roles for u-turn/ventral veinless in the development of Drosophila PNS. [FBrf0158891] Brodu et al., 2002, Development 129(12): 2957--2963 abdominal A specifies one cell type in Drosophila by regulating one principal target gene. [FBrf0148967] Morales et al., 2002, Neuron 34(6): 961--972 Drosophila Fragile X protein, DFXR, regulates neuronal morphology and function in the brain. [FBrf0149142] Wang et al., 2002, Curr. Biol. 12(18): 1611--1616 Drosophila atonal fully rescues the phenotype of Math1 null mice: new functions evolve in new cellular contexts. [FBrf0151846] Hassan et al., 2000, Neuron 25(3): 549--561 atonal regulates neurite arborization but does not act as a proneural gene in the Drosophila brain. [FBrf0127108]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]