Recombinant construct P{EPg}

General Information
Symbol	P{EPg}	FlyBase ID	FBtp0012862
Feature type	transgenic_transposon

Size	8.057	Expression data
Associated insertions	401 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role	mobile activating element (UASG) (Mata et al., 2000)
Description	CV term Qualifiers & info Reference transposon P-element (Mata et al., 2000)
Uses	CV term Qualifiers & info Reference genomic insertion screen mobile activating element UAS (Mata et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	8.057
Left end	P5\' (Mata et al., 2000)
Right end	P3\' (Mata et al., 2000)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001268 3 - pHSS7(N,R1)b 4 + NotI 5 + GAGA_sites 6 + 001271 7 + 14xUAS_GP\T 8 + 001335 9 + EPg_P3'(r,P3')
Reference	(Mata et al., 2000)
Features
	CV term Qualifiers & info Reference 3\' terminus (Levis, 2002.11.20) bacterial selection (Mlodzik and Hiromi, 1992) mobile activating (Levis, 2002.11.20, Mata et al., 2000) partial rescue (Pirrotta, 1988) promoter \| (Levis, 2002.11.20) regulatory/structural DNA (Rorth, 1996, Mata et al., 2000) selectable marker (Pirrotta, 1988, Mata et al., 2000)
Component Alleles
Allele	Scer\UAS^14x.P\T
Reference(s)	(Levis, 2002.11.20, Mata et al., 2000)
Molecular data	Construct: Contains Scer\GAL4 binding sites and the P\T promoter. (Mata et al., 2000)
Phenotypic class
Phenotype manifest in
Other information

Allele	TrlBR^cRa
Reference(s)	(Rorth, 1996, Mata et al., 2000)
Molecular data	Construct: Contains two copies of a 'GAGA element' which can bind Trl. (Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Mata et al., 2000)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{EPg} (Mata et al., 2000)
descendant(s)
Comments
	Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html (Bellen et al., 2011) construct_comment: Similar to P{EP}, but modified to work in the female germline. Used in conjunction with germline-specific driver, P{GAL4::VP16-nos.UTR}, for germline GOF screen. (Mata et al., 2000) report comment: (Mata et al., 2000)
Stocks Listed in FlyBase ( 208 )
Bloomington	21854 w¹¹¹⁸ P{EPg}HP10018 21855 w¹¹¹⁸ P{EPg}HP10020 21856 w¹¹¹⁸ P{EPg}HP10026 21859 w¹¹¹⁸ P{EPg}HP10072 21862 w¹¹¹⁸ P{EPg}HP10119 21864 w¹¹¹⁸ P{EPg}HP10272 21865 P{EPg}HP10277 w¹¹¹⁸ 21874 w¹¹¹⁸ P{EPg}HP10433 21876 w¹¹¹⁸ P{EPg}HP10446 21881 w¹¹¹⁸ P{EPg}HP10668 21884 w¹¹¹⁸ P{EPg}HP10680 21887 w¹¹¹⁸ P{EPg}HP10781
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{EPg} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Cooper et al., 2010, Genetics 185(3): 811--822 Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster. [FBrf0211329] Buchmann et al., 2007, Endocrinology 148(4): 1561--1573 Ablation of the cholesterol transporter adenosine triphosphate-binding cassette transporter G1 reduces adipose cell size and protects against diet-induced obesity. [FBrf0200410] Teleman and Cohen, 2006, Genes Dev. 20(4): 417--422 Drosophila lacking microRNA miR-278 are defective in energy homeostasis. [FBrf0190566] Staudt et al., 2005, PLoS Genet. 1(4): e55 Gain-of-function screen for genes that affect Drosophila muscle pattern formation. [FBrf0191391] Mata et al., 2000, Cell 101(5): 511--522 Tribbles coordinates mitosis and morphogenesis in Drosophila by regulating string/CDC25 proteolysis. [FBrf0128569]
Personal communication to FlyBase	Levis, 2010.6.3, HP30303 and HP31389. HP30303 and HP31389. [FBrf0211661]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]