Recombinant construct P{Mef2-GAL4.247}

General Information
Symbol	P{Mef2-GAL4.247}	FlyBase ID	FBtp0012869
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms smell perception defective
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zars et al., 2000)
Uses	CV term Qualifiers & info Reference characterization Mef2 (Zars et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Zars et al., 2000)
Component Alleles
Allele	Scer\GAL4^Mef2.247
Reference(s)	(Kang et al., 2011, Li et al., 2011, Enell et al., 2010, Awasaki et al., 2011, Acebes et al., 2012, Houot et al., 2012, Sarthi and Elefant, 2011, Tan et al., 2010, Hekmat-Scafe et al., 2010, Wang et al., 2007, Baier et al., 2002, Schwaerzel et al., 2003, Baker et al., 2007, Kim et al., 2007, Zars et al., 2000, McGuire et al., 2001, Pitman et al., 2006, Isabel et al., 2004, McGuire et al., 2003, Ramaekers et al., 2005, Ashraf et al., 2006, Didelot et al., 2006, Robin et al., 2007, Grosjean et al., 2008, Poeck et al., 2008, Tzortzopoulos and Skoulakis, 2007, Yu et al., 2009, Brembs, 2009, Iliadi et al., 2008, Keleman et al., 2007, Krashes et al., 2007, Krashes et al., 2009, Scantlebury et al., 2010, Tanaka et al., 2008, Moressis et al., 2009, Lee and Wu, 2010, Knapek et al., 2011, Goguel et al., 2011, Acebes et al., 2011, Bang et al., 2011, Zhao et al., 2009, Donlea et al., 2009, Hou et al., 2011, Copf et al., 2011, Song et al., 2009, Christiansen et al., 2011, Ferris et al., 2006, Gouzi et al., 2011, Urizar et al., 2007, Zhang et al., 2007, Blum et al., 2009, Xia and Tully, 2007, Sudhakaran et al., 2012, Majumdar et al., 2012, Moraru et al., 2012, Carhan et al., 2005, Aso et al., 2009, Mosca et al., 2012, Zanini et al., 2012, Pfeiffenberger and Allada, 2012, Pauls et al., 2010, Pauls et al., 2010, Michels et al., 2011, Krüttner et al., 2012, Volders et al., 2012, Kaun et al., 2011, Chen et al., 2008, Ellis et al., 2011)
Molecular data	Construct: Scer\GAL4 expression is guided by a 247bp enhancer from a 2.2kb region 15kb upstream of Mef2. (Zars et al., 2000)
Phenotypic class	behavior defective \| heat sensitive, with shi^1.Scer\UAS (Pitman et al., 2006) behavior defective \| male, with Ctet\TeTxLC^{IMPTNT-Q.Scer\UAS} (Baier et al., 2002) behavior defective \| male, with Ctet\TeTxLC^TNT.Scer\UAS (Baier et al., 2002) courtship behavior defective, with qtc^GD8202 (Houot et al., 2012) courtship behavior defective, with Sh^GD13718 (Houot et al., 2012) learning defective, with drk^{dsRNA.Sym.Scer\UAS} (Moressis et al., 2009) lethal, with pum^Scer\UAS.cSa (Chen et al., 2008) lethal \| pupal stage, with Tip60^{dsRNA.Z.Scer\UAS} (Sarthi and Elefant, 2011) lethal \| pupal stage, with Tip60^{E431Q.Scer\UAS} (Sarthi and Elefant, 2011) memory defective, with brp^{dsRNA.B.Scer\UAS}, brp^{dsRNA.C.Scer\UAS} (Knapek et al., 2011) memory defective, with nemy^GD1646 (Iliadi et al., 2008) memory defective, with Phm^GD506 (Iliadi et al., 2008) memory defective, with Tequila^{dsRNA.Scer\UAS} (Didelot et al., 2006) memory defective \| heat sensitive, with shi^1.Scer\UAS (McGuire et al., 2001, Schwaerzel et al., 2003) neuroanatomy defective, with Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} (Acebes et al., 2011) neuroanatomy defective, with Ten-m^GD3620 (Mosca et al., 2012) neuroanatomy defective \| larval stage, with Tip60^{dsRNA.Z.Scer\UAS} (Sarthi and Elefant, 2011) neuroanatomy defective \| larval stage, with Tip60^{E431Q.Scer\UAS} (Sarthi and Elefant, 2011) smell perception defective \| heat sensitive, with Scer\GAL4^Tab2-201Y, shi^1.Scer\UAS (Kaun et al., 2011) temperature response defective \| adult stage, with Cyp6a17^{dsRNA.Scer\UAS.WIZ} (Kang et al., 2011) temperature response defective \| adult stage, with Mmus\Pkaca^mC.Scer\UAS (Kang et al., 2011)
Phenotype manifest in	intrinsic neuron, with Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} (Acebes et al., 2011) mushroom body, with sgg^Scer\UAS.cBa (Acebes et al., 2012) NMJ bouton, with Ten-m^GD3620 (Mosca et al., 2012) synapse, with Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} (Acebes et al., 2011) terminal bouton, with Tip60^{dsRNA.Z.Scer\UAS} (Sarthi and Elefant, 2011) terminal bouton, with Tip60^{E431Q.Scer\UAS} (Sarthi and Elefant, 2011) type I bouton \| ectopic, with Tip60^{dsRNA.Z.Scer\UAS} (Sarthi and Elefant, 2011) type I bouton \| ectopic, with Tip60^{E431Q.Scer\UAS} (Sarthi and Elefant, 2011) type Ib terminal bouton \| ectopic, with Tip60^{dsRNA.Z.Scer\UAS} (Sarthi and Elefant, 2011) type Ib terminal bouton \| ectopic, with Tip60^{E431Q.Scer\UAS} (Sarthi and Elefant, 2011) type Is terminal bouton, with Tip60^{dsRNA.Z.Scer\UAS} (Sarthi and Elefant, 2011) type Is terminal bouton, with Tip60^{E431Q.Scer\UAS} (Sarthi and Elefant, 2011)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference adult stage mushroom body alpha-lobe (Krashes et al., 2007, Tan et al., 2010, Zhao et al., 2009) mushroom body beta-lobe (Krashes et al., 2007, Tan et al., 2010, Zhao et al., 2009) gamma Kenyon cell (Tanaka et al., 2008) calyx of adult mushroom body (Tanaka et al., 2008) mushroom body accessory calyx (Tanaka et al., 2008) mushroom body beta lobe posterior stratum (Tanaka et al., 2008) mushroom body beta lobe surface stratum (Tanaka et al., 2008) mushroom body alpha lobe posterior stratum (Tanaka et al., 2008) mushroom body alpha lobe surface stratum (Tanaka et al., 2008) adult mushroom body \| restricted (Zhao et al., 2009) ellipsoid body (Poeck et al., 2008) nodulus (Poeck et al., 2008) fan-shaped body \| faint & restricted (Poeck et al., 2008) ellipsoid body outer ring \| faint & restricted (Poeck et al., 2008) adult CNS glial cell of second optic chiasm \| restricted (Poeck et al., 2008) adult mushroom body (Poeck et al., 2008, McGuire et al., 2001, Baier et al., 2002, Zars et al., 2000) adult mushroom body gamma-lobe (Aso et al., 2009, Tan et al., 2010, Zhao et al., 2009, Krashes et al., 2007) adult mushroom body beta'-lobe \| restricted (Krashes et al., 2007) adult mushroom body beta'-lobe \| faint (Zhao et al., 2009) adult mushroom body alpha'-lobe \| faint (Zhao et al., 2009) adult mushroom body beta'-lobe (Tan et al., 2010) adult mushroom body alpha'-lobe (Tan et al., 2010) adult mushroom body alpha'-lobe \| restricted (Krashes et al., 2007) mushroom body beta lobe surface stratum \| intense (Aso et al., 2009) mushroom body alpha lobe surface stratum \| intense (Aso et al., 2009) mushroom body beta lobe posterior stratum \| intense (Aso et al., 2009) mushroom body alpha lobe posterior stratum \| intense (Aso et al., 2009) mushroom body beta lobe core stratum (Aso et al., 2009) mushroom body alpha lobe core stratum (Aso et al., 2009) lobula plate \| faint (Aso et al., 2009) adult subesophageal ganglion \| faint (Aso et al., 2009) glial cell of adult brain (Aso et al., 2009)
Additional Information
Statement Reference Expression is found in only a few alpha'/beta' neurons. (Krashes et al., 2007) In the mushroom body, Scer\GAL4[Mef2.247] drives expression in the whole of the alpha and beta lobes, with stronger expression being detected in the surface and posterior subdivisions. In the gamma lobe, the main body of the lobe stains stronger than the dorsomedial tip. (Aso et al., 2009) Expression of Scer\GAL4[Mef2.247] is stronger in the alpha/beta and gamma mushroom body lobes than in the alpha'/beta' mushroom body lobes. (Tan et al., 2010)

Marker for	mushroom body (Baier et al., 2002)
Reflects expression of	Mef2 (McGuire et al., 2001, Baier et al., 2002, Zars et al., 2000)
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	247 (McGuire et al., 2001, Zars et al., 2000, Pitman et al., 2006, McGuire et al., 2003, Urizar et al., 2007, Zhang et al., 2007, Blum et al., 2009, Xia and Tully, 2007, Wang et al., 2007, Chen et al., 2008) 247-Gal4 (Schwaerzel et al., 2003, Zhao et al., 2009, Krüttner et al., 2012, Pfeiffenberger and Allada, 2012) 247-GAL4 (Ashraf et al., 2006, Keleman et al., 2007, Scantlebury et al., 2010, Tan et al., 2010) Gal4-MB247 (Iliadi et al., 2008) mb247 (Baier et al., 2002, Pauls et al., 2010, Christiansen et al., 2011) MB247 (Isabel et al., 2004, Ramaekers et al., 2005, Didelot et al., 2006, Baker et al., 2007, Copf et al., 2011, Brembs, 2009, Volders et al., 2012, Krashes et al., 2007, Krashes et al., 2009, Tanaka et al., 2008, Moressis et al., 2009, Hekmat-Scafe et al., 2010, Knapek et al., 2011, Goguel et al., 2011, Bang et al., 2011, Zhao et al., 2009, Pauls et al., 2010, Kang et al., 2011, Acebes et al., 2012, Pauls et al., 2010, Kaun et al., 2011, Carhan et al., 2005, Aso et al., 2009) MB247G4 (Krüttner et al., 2012) MB247-GAL4 (Kim et al., 2007, Tzortzopoulos and Skoulakis, 2007, Yu et al., 2009, Awasaki et al., 2011, Sudhakaran et al., 2012) MB247Gal4 (Grosjean et al., 2008) mb247-GAL4 (Poeck et al., 2008) MB247-Gal4 (Song et al., 2009, Scantlebury et al., 2010, Acebes et al., 2011, Gouzi et al., 2011, Enell et al., 2010, Houot et al., 2012, Majumdar et al., 2012, Moraru et al., 2012) mb247-Gal4 (Hou et al., 2011, Michels et al., 2011) MB247 GAL4 (Pauls et al., 2010) mef2-GAL4 (Lee and Wu, 2010) Mef2-Gal4 (Sarthi and Elefant, 2011) Mef2-GAL4 (Mosca et al., 2012, Ellis et al., 2011) P247 (Donlea et al., 2009, Ferris et al., 2006, Li et al., 2011) P247-Gal4 (Li et al., 2011) P{Mef2-GAL4.247} (FlyBase, 1996-, Zanini et al., 2012) P^GAL4 mb247 (Robin et al., 2007) P^{Mef2-GAL4.247} (Wang et al., 2007)
Secondary FlyBase IDs

References ( 68 )

Generate a list of	All references relating to this transgenic transposon ( 68 )

List References by type	Research paper ( 63 ) Supplementary material ( 4 ) Review ( 1 )
Recent research papers ( 23 )
	Acebes et al., 2012, J. Neurosci. 32(2): 417--422 Central Adaptation to Odorants Depends on PI3K Levels in Local Interneurons of the Antennal Lobe. [FBrf0217241] Houot et al., 2012, PLoS ONE 7(1): e30799 Genes Involved in Sex Pheromone Discrimination in Drosophila melanogaster and Their Background-Dependent Effect. [FBrf0217343] Krüttner et al., 2012, Neuron 76(2): 383--395 Drosophila CPEB Orb2A Mediates Memory Independent of Its RNA-Binding Domain. [FBrf0219767] Majumdar et al., 2012, Cell 148(3): 515--529 Critical Role of Amyloid-like Oligomers of Drosophila Orb2 in the Persistence of Memory. [FBrf0217407] Moraru et al., 2012, Neural Dev. 7(1): 14 Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila. [FBrf0218691] Pfeiffenberger and Allada, 2012, PLoS Genet. 8(10): e1003003 Cul3 and the BTB Adaptor Insomniac Are Key Regulators of Sleep Homeostasis and a Dopamine Arousal Pathway in Drosophila. [FBrf0219613] Sudhakaran et al., 2012, J. Neurosci. 32(21): 7225--7231 Plasticity of recurrent inhibition in the Drosophila antennal lobe. [FBrf0218409] Volders et al., 2012, J. Neurosci. 32(43): 15193--15204 Drosophila rugose Is a Functional Homolog of Mammalian Neurobeachin and Affects Synaptic Architecture, Brain Morphology, and Associative Learning. [FBrf0219807] Zanini et al., 2012, Genes Brain Behav. 11(7): 819--827 Deletion of the Drosophila neuronal gene found in neurons disrupts brain anatomy and male courtship. [FBrf0219541] Acebes et al., 2011, J. Neurosci. 31(8): 2734--2745 Synapse loss in olfactory local interneurons modifies perception. [FBrf0213264] Bang et al., 2011, PLoS Genet. 7(3): e1001346 Dopamine signalling in mushroom bodies regulates temperature-preference behaviour in Drosophila. [FBrf0213392] Christiansen et al., 2011, J. Neurosci. 31(26): 9696--9707 Presynapses in Kenyon Cell Dendrites in the Mushroom Body Calyx of Drosophila. [FBrf0214059] Copf et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(19): 8059--8064 Cytokine signaling through the JAK/STAT pathway is required for long-term memory in Drosophila. [FBrf0213703] Ellis et al., 2011, J. Cell Sci. 124(11): 1844--1856 In vivo functional analysis reveals specific roles for the integrin-binding sites of talin. [FBrf0213701] Goguel et al., 2011, J. Neurosci. 31(3): 1032--1037 Drosophila amyloid precursor protein-like is required for long-term memory. [FBrf0212809] Gouzi et al., 2011, PLoS Genet. 7(9): e1002281 The receptor tyrosine kinase alk controls neurofibromin functions in Drosophila growth and learning. [FBrf0216258] Hou et al., 2011, Nitric Oxide 24(1): 17--24 Nitric oxide metabolism controlled by formaldehyde dehydrogenase (fdh, homolog of mammalian GSNOR) plays a crucial role in visual pattern memory in Drosophila. [FBrf0212726] Kang et al., 2011, PLoS ONE 6(12): e29800 Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila. [FBrf0217065] Kaun et al., 2011, Nat. Neurosci. 14(5): 612--619 A Drosophila model for alcohol reward. [FBrf0213574] Knapek et al., 2011, J. Neurosci. 31(9): 3453--3458 Bruchpilot, a synaptic active zone protein for anesthesia-resistant memory. [FBrf0213189] Li et al., 2011, PLoS ONE 6(11): e28269 The hector G-Protein Coupled Receptor Is Required in a Subset of fruitless Neurons for Male Courtship Behavior. [FBrf0216834] Michels et al., 2011, Learn. Mem. 18(5): 332--344 Cellular site and molecular mode of synapsin action in associative learning. [FBrf0213538] Sarthi and Elefant, 2011, PLoS ONE 6(10): e26202 dTip60 HAT Activity Controls Synaptic Bouton Expansion at the Drosophila Neuromuscular Junction. [FBrf0216592] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...