Recombinant construct M{3xP3-EGFP}

General Information
Symbol	M{3xP3-EGFP}	FlyBase ID	FBtp0012982
Feature type	transgenic_transposon	Created / Updated	2000-11-16/2001-05-01

Size		Expression data	GFP reporter
Location-dependent role		Associated insertions	0 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon Dmau\mariner (Berghammer et al., 1999, Horn et al., 2000)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Berghammer et al., 1999, Horn et al., 2000)
Component Alleles
Avic\GFP^E.3xP3
Reference(s)	(Lozovsky et al., 2002, Berghammer et al., 1999, Horn et al., 2000)
Molecular data	Construct: Artificial promoter containing three binding sites for Mmus\Pax6 homodimers in front of a TATA box drives the expression of the enhanced variant of Avic\GFP (EGFP). (Berghammer et al., 1999) Three tandem repeats of an artificially derived Mmus\Pax6 homodimer binding site are fused upstream of a TATA box driving expression of "EGFP". (Horn et al., 2000)
Phenotypic class
Phenotype manifest in
Other information
Dmau\mariner\T^SalG
Reference(s)	(Lozovsky et al., 2002)
Molecular data	Construct: Insertion of 1.3kb fragment containing Avic\GFP^E.3xP3 in the SalI site at position 349 of the M{Mos1} element. The Avic\GFP^E.3xP3 marker gene is transcribed in the opposite orientation to the Dmau\mariner\T open reading frame. (Lozovsky et al., 2002)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference adult stage ocellus (Horn et al., 2000) larval stage anal pad (Horn et al., 2000) larval stage larval central nervous system (Horn et al., 2000) larval stage larval sense organ (Horn et al., 2000) pupal stage \| late eye (Horn et al., 2000) larval stage Bolwig\\\'s organ (Horn et al., 2000) larval stage larval hindgut (Horn et al., 2000) adult stage eye (Horn et al., 2000) embryonic stage \| stage >=16 Bolwig\\\'s organ \| presumptive (Horn et al., 2000)
Additional Information
Statement Reference

Assay mode	distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000)
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pM{3xP3-EGFP} (Horn et al., 2000)
descendant(s)
Comments
	construct_comment: One of several constructs used to test feasibilty of using Avic\GFP as a \'universal marker.\' Tested in D. melanogaster and Tribolium castaneum. (Berghammer et al., 1999) construct_comment: Non-autonomous; Dmau\mariner\T disrupted.Tested in D.melanogaster. (Horn et al., 2000) report comment: GFP expression driven by multimer of an idealized ey/Mmus\Pax6 binding site (P3), resulting in high levels of expression in the eye. (Horn et al., 2000)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	Dmau\mariner{3xP3-EGFP} (FlyBase, 1996-) M{3xP3-EGFP} (FlyBase, 1996-) Mos{3xP3-EGFP} (Horn et al., 2000) SalG (Lozovsky et al., 2002)
Secondary FlyBase IDs

References ( 4 )
Research paper	Lozovsky et al., 2002, Genetics 160(2): 527--535 Unexpected Stability of mariner Transgenes in Drosophila. [FBrf0144896] Horn et al., 2000, Dev. Genes Evol. 210(12): 623--629 Highly sensitive, fluorescent transformation marker for Drosophila transgenesis. [FBrf0132292] Berghammer et al., 1999, Nature 402(6760): 370--371 A universal marker for transgenic insects. [FBrf0122963]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]