Recombinant construct P{70I-SceI}

General Information
Symbol	P{70I-SceI}	FlyBase ID	FBtp0012998
Feature type	transgenic_transposon	Created / Updated	2000-11-16/2000-12-22

Size		Expression data
Location-dependent role		Associated insertions	2 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Rong and Golic, 2000)
Uses	CV term Qualifiers & info Reference enzyme source Scer\SCEI (Rong and Golic, 2000) heat-shock construct Scer\SCEI (Rong and Golic, 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Rong and Golic, 2000) inducible promoter (Rong and Golic, 2000) chimeric gene (Rong and Golic, 2000)
Component Alleles
v^+t1.8
Reference(s)	(Rong and Golic, 2000)
Molecular data	Construct: 1.8kb SspI fragment that contains v function. 1.8kb fragment replaces the 7.2kb HindIII ry fragment of pDM30. (Fridell and Searles, 1991)
Phenotypic class	wild-type (Fridell and Searles, 1991)
Phenotype manifest in
Other information
Scer\SCEI^hs.PR
Reference(s)	(Rong and Golic, 2000, Kracklauer et al., 2007, Watnick et al., 2003, Gao et al., 2003, Seum et al., 2002, Rong and Golic, 2001, Rong and Golic, 2003, Gao et al., 2003, Watnick et al., 2003, Lankenau et al., 2003, Sogame et al., 2003, Gong and Golic, 2003, LaLonde et al., 2006)
Molecular data	Construct: The hsp70 promoter and 3' sequences flank the Scer\SCEI coding sequence. (Rong and Golic, 2000)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{70I-SceI} (Rong and Golic, 2000)
descendant(s)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	70I-SceI (Gao et al., 2003, Watnick et al., 2003, Gao et al., 2003, Watnick et al., 2003, Sogame et al., 2003, Gong and Golic, 2003, Rong and Golic, 2001, Rong and Golic, 2000, LaLonde et al., 2006, Rong and Golic, 2003) hs-I-SceI (Kracklauer et al., 2007) hs-ISce-I (Kracklauer et al., 2007) P[v⁺,70ISceI] (Seum et al., 2002) P [v⁺, 70I-SceI] (LaLonde et al., 2006) P{70I-SceI} (FlyBase, 1996-) P{@Scer\SCEI^hs.PR@ @v^+t1.8@=@70I-SceI@} (FlyBase, 1996-, Rong and Golic, 2000) P{v⁺, 70I-SceI} (Rong and Golic, 2000) P{v⁺,70I-SceI} (Lankenau et al., 2003)
Secondary FlyBase IDs

References ( 15 )
Research paper	Kracklauer et al., 2007, Fly 1(2): 75--85 Drosophila klaroid Encodes a SUN Domain Protein Required for Klarsicht Localization to the Nuclear Envelope and Nuclear Migration in the Eye. [FBrf0200125] LaLonde et al., 2006, BMC Dev. Biol. 6: A role for Phospholipase D in Drosophila embryonic cellularization. [FBrf0198770] Gao et al., 2003, Curr. Biol. 13(24): 2175--2178 PKD2 cation channel is required for directional sperm movement and male fertility. [FBrf0167414] Gong and Golic, 2003, Proc. Natl. Acad. Sci. USA 100(5): 2556--2561 Ends-out, or replacement, gene targeting in Drosophila. [FBrf0159285] Lankenau et al., 2003, Genetics 163(2): 611--623 Knockout targeting of the Drosophila Nap1 gene and examination of DNA repair tracts in the recombination products. [FBrf0158984] Rong and Golic, 2003, Genetics 165(4): 1831--1842 The homologous chromosome is an effective template for the repair of mitotic DNA double-strand breaks in Drosophila. [FBrf0167645] Sogame et al., 2003, Proc. Natl. Acad. Sci. USA 100(8): 4696--4701 Drosophila p53 preserves genomic stability by regulating cell death. [FBrf0159299] Watnick et al., 2003, Curr. Biol. 13(24): 2179--2184 A flagellar polycystin-2 homolog required for male fertility in Drosophila. [FBrf0167415] Seum et al., 2002, Genetics 161(3): 1125--1136 Isolation of Su(var)3-7 mutations by homologous recombination in Drosophila melanogaster. [FBrf0151329] Rong and Golic, 2001, Genetics 157(3): 1307--1312 A targeted gene knockout in Drosophila. [FBrf0134649] Rong and Golic, 2000, Science 288(5473): 2013--2018 Gene targeting by homologous recombination in Drosophila. [FBrf0128625] Fridell and Searles, 1991, Nucleic Acids Res. 19: 5082 Vermilion as a small selectable marker gene for Drosophila transformation. [FBrf0054853]
Supplementary material	Gao et al., 2003, Curr. Biol. 13(24): PKD2 cation channel is required for directional sperm movement and male fertility. [FBrf0178917] Watnick et al., 2003, Curr. Biol. 13(24): A flagellar polycystin-2 homolog required for male fertility in Drosophila. [FBrf0178959]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]