Recombinant construct P{UAS-Hep.Act}

General Information
Symbol	P{UAS-Hep.Act}	FlyBase ID	FBtp0013343
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Weber et al., 2000)
Uses	CV term Qualifiers & info Reference binary system (targeted) hep (Weber et al., 2000) characterization hep (Weber et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Weber et al., 2000) selectable marker (Weber et al., 2000)
Component Alleles
Allele	hep^Act.Scer\UAS
Reference(s)	(Wang et al., 2005, Munoz-Descalzo et al., 2005, Uhlirova et al., 2005, Jasper et al., 2001, Lee et al., 2005, Brumby and Richardson, 2003, Weber et al., 2000, Cha et al., 2005, Karpac et al., 2009, Hull-Thompson et al., 2009, Uhlirova and Bohmann, 2006, Hueber et al., 2007, Mathieu et al., 2007, Rodahl et al., 2009, Srivastava et al., 2007, Lee et al., 2006, Rousset et al., 2010, Gettings et al., 2010, Blanco et al., 2010, Gonda et al., 2012, Belacortu et al., 2011)
Molecular data	Construct: Amino acid replacement: S326D. Amino acid replacement: T330D. Expression of the hep mutant coding region is governed by Scer\UAS. (Weber et al., 2000)
Phenotypic class	cell lethal \| partially \| somatic clone, with Scer\GAL4^αTub84B.PL (Brumby and Richardson, 2003) cell shape defective \| embryonic stage, with Scer\GAL4^arm.PS (Hueber et al., 2007) heat stress response defective \| adult stage (Gonda et al., 2012) lethal, with Scer\GAL4^69B (Rousset et al., 2010) lethal \| embryonic stage, with Scer\GAL4^69B (Munoz-Descalzo et al., 2005) planar polarity defective, with Scer\GAL4^hs.2sev (Weber et al., 2000) small body \| adult stage, with Scer\GAL4^Ilp2.PR (Wang et al., 2005) visible, with Scer\GAL4^hs.2sev (Weber et al., 2000)
Phenotype manifest in	embryonic/first instar larval cuticle, with Scer\GAL4^69B (Rousset et al., 2010) embryonic/first instar larval cuticle, with Scer\GAL4^arm.PS (Jasper et al., 2001) embryonic leading edge cell, with Scer\GAL4^ptc-559.1 (Gettings et al., 2010) embryonic maxillary segment, with Scer\GAL4^arm.PS (Hueber et al., 2007) eye, with Scer\GAL4^hs.2sev (Weber et al., 2000) eye, with Scer\GAL4^sev.EP (Wang et al., 2005) eye disc, with Scer\GAL4^hs.2sev (Weber et al., 2000) ommatidial precursor cluster, with Scer\GAL4^hs.2sev (Weber et al., 2000)
Other information

Allele	w^+mC
Reference(s)	(Weber et al., 2000)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 2 )
Bloomington	9306 w^; P{UAS-Hep.Act}2 9305 P{UAS-Hep.Act}1, w^/FM6, w^*
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	hep^CA (Srivastava et al., 2007) P{UAS-Hep.Act} (FlyBase, 1996-) UAS-Hemipterous (Lee et al., 2006) UAS-hep (Cha et al., 2005) UAS-Hep (Lee et al., 2006) UAS-hep^act (Lee et al., 2005, Hueber et al., 2007, Rousset et al., 2010, Gettings et al., 2010, Gonda et al., 2012) UAS-hep^ACT (Uhlirova et al., 2005, Brumby and Richardson, 2003, Mathieu et al., 2007) UAShep^act (Jasper et al., 2001) UASHep^act (Karpac et al., 2009) UAS-Hep^act (Hull-Thompson et al., 2009) UAS-Hep^ACT (Belacortu et al., 2011) UAS-hep^CA (Blanco et al., 2010) UASHepact (Wang et al., 2005) UAS-Hep-act (Rodahl et al., 2009)
Secondary FlyBase IDs

References ( 21 )

Generate a list of	All references relating to this transgenic transposon ( 21 )

List References by type	Research paper ( 21 )
Recent research papers ( 2 )
	Gonda et al., 2012, PLoS ONE 7(7): e42369 Drosophila Heat Shock Response Requires the JNK Pathway and Phosphorylation of Mixed Lineage Kinase at a Conserved Serine-Proline Motif. [FBrf0219015] Belacortu et al., 2011, Gene Expr. Patterns 11(3-4): 190--201 Expression of Drosophila Cabut during early embryogenesis, dorsal closure and nervous system development. [FBrf0213309] Show all research papers ...