Recombinant construct P{UASp-GFPS65C-αTub84B}

General Information
Symbol	P{UASp-GFPS65C-αTub84B}	FlyBase ID	FBtp0013497
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Grieder et al., 2000)
Uses	CV term Qualifiers & info Reference binary system (targeted) alphaTub84B (Grieder et al., 2000) characterization alphaTub84B (Grieder et al., 2000) reporter construct vital \| alphaTub84B (Grieder et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Grieder et al., 2000) molecular tag (Grieder et al., 2000) selectable marker (Grieder et al., 2000)
Component Alleles
Allele	w^+mC
Reference(s)	(Grieder et al., 2000)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	αTub84B^{Scer\UAS.P\T.T:Avic\GFP-S65C}
Reference(s)	(Savoian and Rieder, 2002, Fei et al., 2002, Schneider et al., 2006, Timinszky et al., 2002, Sanchez-Soriano et al., 2005, Williams and Truman, 2005, Januschke et al., 2002, Trieselmann and Wilde, 2002, Jankovics et al., 2002, Raff et al., 2002, Vaccari and Ephrussi, 2002, Kwon et al., 2004, Megraw et al., 2002, Rogers et al., 2002, Schnorrer et al., 2002, Avidor-Reiss et al., 2004, Tirian et al., 2003, Grieder et al., 2000, Brust-Mascher et al., 2004, Schulz et al., 2004, Yamashita et al., 2003, Yu et al., 2006, Bokel et al., 2005, Wehr et al., 2006, Serbus et al., 2005, Ataman et al., 2006, Ataman, 2006, Oshima, 2006, Basto et al., 2006, Venkei et al., 2006, Frescas et al., 2006, Shimada et al., 2006, Silverman-Gavrila et al., 2008, Leiss et al., 2009, Colombié et al., 2008, Iijima-Ando et al., 2009, Lucas and Raff, 2007, Januschke and Gonzalez, 2010, Hallen et al., 2008, Abuin et al., 2011, Cheng et al., 2011, Jankovics and Brunner, 2006, Jana et al., 2011, Lerit and Gavis, 2011, Lee et al., 2009, Abreu-Blanco et al., 2011, Bechstedt et al., 2010, Dix and Raff, 2007, Dix and Raff, 2007, Liu et al., 2008, MacDonald et al., 2006, Puah et al., 2011, Rahmani et al., 2009, Verdier et al., 2006, Stinchfield et al., 2012, Uchino et al., 2013, Sabino et al., 2011)
Molecular data	Construct: Scer\UAS regulatory and P\T promoter sequences drive expression of the N-terminal region of αTub84B (encompassing amino acid residue 2 up to the internal BamHI site) tagged with T:Avic\GFP^S65C. (Grieder et al., 2000)
Phenotypic class	viable, with Scer\GAL4^nos.PG, Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Grieder et al., 2000)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 3 )
Bloomington	7373 w^; P{UASp-GFPS65C-αTub84B}3/TM3, Sb¹ 7374 y¹ w^; P{UASp-GFPS65C-αTub84B}14-6-II 7253 w¹¹¹⁸; P{GAL4::VP16-nos.UTR}CG6325^MVD1, P{UASp-GFPS65C-αTub84B}3
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	{UASp-GFPS65C-alphaTub84B} (Liu et al., 2008) GFP::tubulin (Brust-Mascher et al., 2004) GFP-tub (Wehr et al., 2006) GFP-tubulin (Kwon et al., 2004, Rogers et al., 2002, Schneider et al., 2006, Frescas et al., 2006, Silverman-Gavrila et al., 2008, Rahmani et al., 2009, Jankovics and Brunner, 2006, Sabino et al., 2011) GFP-α-tubulin (Trieselmann and Wilde, 2002, Dix and Raff, 2007, Savoian and Rieder, 2002, Colombié et al., 2008, Dix and Raff, 2007, Hallen et al., 2008, Lerit and Gavis, 2011, Abreu-Blanco et al., 2011) GFP-α-Tubulin (Basto et al., 2006, Lucas and Raff, 2007) P[UAS-αTub84B-GFP] (Verdier et al., 2006) p[w⁺.UASp-GFPS65C-α1-tub84B] (Grieder et al., 2000) P{UAS-GFPS65C-αTub84B} (FlyBase, 1996-) P{UASp-GFPS65C-alphaTub84B} (Abreu-Blanco et al., 2011) P{UASp-GFPS65C-αTub84B} (FlyBase, 1996-) P{w⁺ UASp-GFPS65c-αTub84B} (Serbus et al., 2005) pUASp::GFP65S-αTubulin84B (Bokel et al., 2005) tub-GFP (Schulz et al., 2004, Iijima-Ando et al., 2009) tubulin::GFP (Williams and Truman, 2005) Tubulin:GFP (Jankovics et al., 2002) tubulin-GFP (Tirian et al., 2003, Raff et al., 2002, Iijima-Ando et al., 2009, Januschke and Gonzalez, 2010, Puah et al., 2011) UAS-GFP::tubulin (Shimada et al., 2006) UAS-GFP:α1tub84B (Abuin et al., 2011) UAS-GFP-Tubulin (Bechstedt et al., 2010) UAS-GFP-α1tub84B (Avidor-Reiss et al., 2004) UAS-GFP-α-tub84B (Sanchez-Soriano et al., 2005) UAS-GFP-α-tubulin (Yamashita et al., 2003, Oshima, 2006, Cheng et al., 2011) UASp.GFP-αTub84B (Stinchfield et al., 2012) UASp-GFPS65C-α1-tub84B (Januschke et al., 2002) UASp-GFP-α-tubulin (Colombié et al., 2008) UAS-tub-GFP (Lee et al., 2009, Iijima-Ando et al., 2009) UAS-tubulin84B::GFP (Jana et al., 2011) UAS-tubulin-GFP (Timinszky et al., 2002, Leiss et al., 2009, Uchino et al., 2013) UAS-Tubulin-GFP (Ataman et al., 2006, Ataman, 2006) UAS-α-tubulin::GFP (MacDonald et al., 2006) UAS-α-tubulin-GFP (Fei et al., 2002) α1-tubulin-GFP (Venkei et al., 2006) α-TubGFP (Schnorrer et al., 2002) α-tubulin-GFP (Vaccari and Ephrussi, 2002, Yu et al., 2006)
Secondary FlyBase IDs

References ( 58 )

Generate a list of	All references relating to this transgenic transposon ( 58 )

List References by type	Research paper ( 54 ) Supplementary material ( 4 )
Recent research papers ( 9 )
	Uchino et al., 2013, Dev. Biol. 373(1): 216--227 Loss of Drosophila A-type lamin C initially causes tendon abnormality including disintegration of cytoskeleton and nuclear lamina in muscular defects. [FBrf0220052] Stinchfield et al., 2012, Development 139(15): 2721--2729 Fat facets deubiquitylation of Medea/Smad4 modulates interpretation of a Dpp morphogen gradient. [FBrf0219593] Abreu-Blanco et al., 2011, J. Cell Biol. 193(3): 455--464 Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling. [FBrf0213583] Abuin et al., 2011, Neuron 69(1): 44--60 Functional architecture of olfactory ionotropic glutamate receptors. [FBrf0212725] Cheng et al., 2011, Development 138(5): 831--837 Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning. [FBrf0213009] Jana et al., 2011, Mol. Biol. Cell 22(6): 769--781 Heterotrimeric kinesin-II is necessary and sufficient to promote different stepwise assembly of morphologically distinct bipartite cilia in Drosophila antenna. [FBrf0213262] Lerit and Gavis, 2011, Curr. Biol. 21(6): 439--448 Transport of germ plasm on astral microtubules directs germ cell development in Drosophila. [FBrf0213255] Puah et al., 2011, BioTechniques 51(1): 49--54 TLM-Converter: reorganization of long time-lapse microscopy datasets for downstream image analysis. [FBrf0214451] Sabino et al., 2011, J. Cell Sci. 124(7): 1156--1166 Drosophila Ajuba is not an Aurora-A activator but is required to maintain Aurora-A at the centrosome. [FBrf0213223] Show all research papers ...