Recombinant construct P{UAS-robo2.HA1}

General Information
Symbol	P{UAS-robo2.HA1}	FlyBase ID	FBtp0013813
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Rajagopalan et al., 2000)
Uses	CV term Qualifiers & info Reference binary system (targeted) robo2 (Rajagopalan et al., 2000) characterization robo2 (Rajagopalan et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Rajagopalan et al., 2000) molecular tag (Rajagopalan et al., 2000) selectable marker (Rajagopalan et al., 2000)
Component Alleles
Allele	lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg}
Reference(s)	(Qian et al., 2005, Englund et al., 2002, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Parsons et al., 2003, Grueber et al., 2007)
Molecular data	Scer\UAS regulatory sequences drive expression of lea cDNA sequences in which the 5' and 3' untranslated regions have been removed and the 5' sequences encoding the signal sequence have been replaced with sequences encoding the wg signal sequence (T:SS-wg) followed by three copies of the T:Ivir\HA1 tag. (Rajagopalan et al., 2000)
Phenotypic class
Phenotype manifest in	embryonic/larval neuron, with Scer\GAL4^ap-md544 (Rajagopalan et al., 2000) ganglionic branch primordium, with Scer\GAL4^bs.Term (Englund et al., 2002) ventral nerve cord commissure, with Scer\GAL4^elav.PLu (Rajagopalan et al., 2000)
Other information

Allele	w^+mC
Reference(s)	(Rajagopalan et al., 2000)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-robo2.HA1} (FlyBase, 1996-) UAS-HA-robo2 (Rajagopalan et al., 2000) UAS-robo2 (Qian et al., 2005, Parsons et al., 2003, Rajagopalan et al., 2000, Grueber et al., 2007) UAS-robo2HA (Englund et al., 2002)
Secondary FlyBase IDs

References ( 6 )
Research paper	Grueber et al., 2007, Development 134(1): 55--64 Projections of Drosophila multidendritic neurons in the central nervous system: links with peripheral dendrite morphology. [FBrf0194377] Qian et al., 2005, Curr. Biol. 15(24): 2271--2278 Slit and Robo control cardiac cell polarity and morphogenesis. [FBrf0190040] Parsons et al., 2003, Dev. Biol. 264(2): 363--375 roundabout gene family functions during sensory axon guidance in the Drosophila embryo are mediated by both Slit-dependent and Slit-independent mechanisms. [FBrf0167462] Englund et al., 2002, Development 129(21): 4941--4951 Attractive and repulsive functions of Slit are mediated by different receptors in the Drosophila trachea. [FBrf0151931] Rajagopalan et al., 2000, Neuron 28(3): 767--777 Crossing the midline: roles and regulation of Robo receptors. [FBrf0132432] Rajagopalan et al., 2000, Cell 103(7): 1033--1045 Selecting a longitudinal pathway: robo receptors specify the lateral position of axons in the Drosophila CNS. [FBrf0132258]