Recombinant construct P{UAS-WASp.B}

General Information
Symbol	P{UAS-WASp.B}	FlyBase ID	FBtp0013909
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Ben-Yaacov et al., 2001)
Uses	CV term Qualifiers & info Reference binary system (targeted) WASp (Ben-Yaacov et al., 2001) characterization WASp (Ben-Yaacov et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Ben-Yaacov et al., 2001) selectable marker (Ben-Yaacov et al., 2001)
Component Alleles
Allele	w^+mC
Reference(s)	(Ben-Yaacov et al., 2001)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	WASp^Scer\UAS.cBa
Reference(s)	(Ben-Yaacov et al., 2001, Tal et al., 2002, Coyle et al., 2004, Rotkopf et al., 2011, Nahm et al., 2010, Schafer et al., 2007, Khuong et al., 2010, Duboff et al., 2012)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of a full length WASp cDNA. (Ben-Yaacov et al., 2001)
Phenotypic class
Phenotype manifest in	adult brain, with Scer\GAL4^elav.PU (Duboff et al., 2012) mitochondrion, with Scer\GAL4^elav.PU (Duboff et al., 2012)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	39724 y¹ w^*; P{UAS-WASp.B}25
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-WASp.B} (FlyBase, 1996-) UAS-wasp (Schafer et al., 2007) UAS-WASP (Duboff et al., 2012) UAS-Wsp (Tal et al., 2002, Rotkopf et al., 2011) UAS-WSP (Khuong et al., 2010) UAS-wsp (Nahm et al., 2010) UAS-wsp⁺ (Coyle et al., 2004) UASwsp^fl (Khuong et al., 2010) WSP^fl (Khuong et al., 2010)
Secondary FlyBase IDs

References ( 9 )
Research paper	Duboff et al., 2012, Neuron 75(4): 618--632 Tau Promotes Neurodegeneration via DRP1 Mislocalization In Vivo. [FBrf0219271] Rotkopf et al., 2011, Development 138(13): 2729--2739 The WASp-based actin polymerization machinery is required in somatic support cells for spermatid maturation and release. [FBrf0213892] Khuong et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(40): 17379--17384 WASP is activated by phosphatidylinositol-4,5-bisphosphate to restrict synapse growth in a pathway parallel to bone morphogenetic protein signaling. [FBrf0214154] Nahm et al., 2010, J. Cell Biol. 191(3): 661--675 The Cdc42-selective GAP Rich regulates postsynaptic development and retrograde BMP transsynaptic signaling. [FBrf0212218] Schafer et al., 2007, Dev. Biol. 304(2): 664--674 The Wiskott-Aldrich syndrome protein (WASP) is essential for myoblast fusion in Drosophila. [FBrf0194614] Coyle et al., 2004, Neuron 41(4): 521--534 Nervous wreck, an SH3 adaptor protein that interacts with Wsp, regulates synaptic growth in Drosophila. [FBrf0175021] Tal et al., 2002, Dev. Biol. 243(2): 260--271 Conserved interactions with cytoskeletal but not signaling elements are an essential aspect of Drosophila WASp function. [FBrf0144805] Ben-Yaacov et al., 2001, J. Cell Biol. 152(1): 1--14 Wasp, the Drosophila Wiskott-Aldrich Syndrome gene homologue, is required for cell fate decisions mediated by Notch signaling. [FBrf0132374]
Personal communication to FlyBase	Bellen, 2012.5.6, Bellen insertions. Bellen insertions. [FBrf0219492]