Recombinant construct P{ACE3(-FRT)Ori-β}

General Information
Symbol	P{ACE3(-FRT)Ori-β}	FlyBase ID	FBtp0014047
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Lu et al., 2001)
Uses	CV term Qualifiers & info Reference characterization ACE3 (Lu et al., 2001) Ori66Dbeta (Lu et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference regulatory/structural DNA (Lu et al., 2001) selectable marker (Lu et al., 2001)
Component Alleles
Allele	ACE3³²⁰
Reference(s)	(Lu et al., 2001)
Molecular data	320bp fragment. (Lu et al., 2001)
Phenotypic class
Phenotype manifest in
Other information

Allele	gypsy\su(Hw)BR⁴³⁰
Reference(s)	(Lu et al., 2001)
Molecular data	Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon. (Kuhn et al., 2003) Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014). (Roseman et al., 1995) Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014. (Roseman et al., 1993) Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites. (Patton et al., 1992)
Phenotypic class
Phenotype manifest in
Other information

Allele	Ori66Dβ⁸⁸⁴
Reference(s)	(Lu et al., 2001)
Molecular data	884 bp fragment of Ori66Dβ. (Lu et al., 2001)
Phenotypic class
Phenotype manifest in
Other information

Allele	y^+mDint2
Reference(s)	(Lu et al., 2001)
Molecular data	Construct: 5.2kb y⁺ gene including 2.8kb of 5' flanking sequences and 0.13kb of 3' sequences and lacks the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5.2kb y gene fragment containing 2.8kb of 5' flanking and 0.13kb of 3' flanking DNA and lacking the intron that is the location of the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5kb intronless SalI fragment containing the y gene. (Harrison and Perrimon, 1993) 5.2kb SalI y gene intronless fragment containing 2.9kb of 5' flanking and 0.3kb of 3' flanking DNA. (Patton et al., 1992)
Phenotypic class	body color defective (Ring and Garza, 2003.6.10, Roseman et al., 2001) visible (Roseman et al., 2001)
Phenotype manifest in	adult cuticle (Roseman et al., 2001) arista (Ring and Garza, 2003.6.10) macrochaeta (Ring and Garza, 2003.6.10) unguis (Ring and Garza, 2003.6.10)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	P{ACE3(FRT.SHWBS)Ori-β} (Lu et al., 2001)
descendant(s)
Comments
	report comment: Contains a single Scer\FRT site. (Lu et al., 2001) construct_comment: The remaining FRT site is flanked by "stuffer" sequences. (Lu et al., 2001)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{(-FRT)SHWBS} (FlyBase, 1996-) P{ACE3(-FRT)Ori-β} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Lu et al., 2001, Genes Dev. 15(2): 134--146 Functionally distinct, sequence-specific replicator and origin elements are required for Drosophila chorion gene amplification. [FBrf0132324]