Recombinant construct PBac{3xP3-ECFPafm}

General Information
Symbol	PBac{3xP3-ECFPafm}	FlyBase ID	FBtp0014059
Feature type	transgenic_transposon	Created / Updated	2001-05-29/2001-06-04

Size		Expression data
Location-dependent role		Associated insertions	0 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon Tni\piggyBac (Horn and Wimmer, 2000)
Uses	CV term Qualifiers & info Reference carrier transposon Tni\piggyBac (Horn and Wimmer, 2000) reporter \| vital (Horn and Wimmer, 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Horn and Wimmer, 2000)
Component Alleles
Avic\GFP^ECFP.3xP3
Reference(s)	(Horn and Wimmer, 2000)
Molecular data	Construct: The "3xP3" promoter sequence (an artificial promoter containing 3 Mmus\Pax6 homodimer binding sites) drives expression of the "ECFP" form ("cyan GFP") of Avic\GFP. (Horn and Wimmer, 2000)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pPBac{3xP3-ECFPafm} (Horn and Wimmer, 2000)
descendant(s)
Comments
	report comment: Modified (relative to PBac{3xP3-ECFPaf}) by introduction of a 0.8kb deletion in transposase ORF. (Horn and Wimmer, 2000) construct_comment: One of a series of transposon cloning vectors using the transposon Tni\piggyBac as the transfection agent and spectral variants of the selectable marker Avic\GFP. (Horn and Wimmer, 2000)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{3xP3-ECFPafm} (FlyBase, 1996-, Horn and Wimmer, 2000)
Secondary FlyBase IDs

References ( 2 )
Research paper	Horn and Wimmer, 2000, Dev. Genes Evol. 210(12): 630--637 A versatile vector set for animal transgenesis. [FBrf0134553]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]